Oxidative-Stress-Mediated Epigenetic Dysregulation in Spermatogenesis: Implications for Male Infertility and Offspring Health
Genes,
Год журнала:
2025,
Номер
16(1), С. 93 - 93
Опубликована: Янв. 17, 2025
Male
reproductive
health
is
governed
by
an
intricate
interplay
of
genetic,
epigenetic,
and
environmental
factors.
Epigenetic
mechanisms—encompassing
DNA
methylation,
histone
modifications,
non-coding
RNA
activity—are
crucial
both
for
spermatogenesis
sperm
maturation.
However,
oxidative
stress,
driven
excessive
reactive
oxygen
species,
disrupts
these
processes,
leading
to
impaired
function
male
infertility.
This
disruption
extends
epigenetic
resulting
in
abnormal
gene
expression
chromatin
remodeling
that
compromise
genomic
integrity
fertilization
potential.
Importantly,
oxidative-stress-induced
alterations
can
be
inherited,
affecting
the
fertility
offspring
future
generations.
review
investigates
how
stress
influences
regulation
reproduction
modifying
RNAs,
ultimately
compromising
spermatogenesis.
Additionally,
it
discusses
transgenerational
implications
disruptions
their
potential
role
hereditary
infertility
disease
predisposition.
Understanding
mechanisms
vital
developing
therapeutic
strategies
mitigate
damage
restore
homeostasis
germline.
By
integrating
insights
from
molecular,
clinical,
research,
this
work
emphasizes
need
targeted
interventions
enhance
prevent
adverse
outcomes
progeny.
Furthermore,
elucidating
dose–response
relationships
between
changes
remains
a
critical
research
priority,
informing
personalized
diagnostics
interventions.
In
context,
studies
should
adopt
standardized
markers
damage,
robust
clinical
trials,
multi-omic
approaches
capture
complexity
Such
rigorous
investigations
will
reduce
risk
disorders
optimize
outcomes.
Язык: Английский
Lyophilized and Oven-Dried Manilkara zapota Extracts: Characterization and In Vitro, In Vivo, and In Silico Analyses
Plants,
Год журнала:
2025,
Номер
14(2), С. 216 - 216
Опубликована: Янв. 14, 2025
In
this
work,
extracts
from
the
pulp,
peel,
and
seed
of
Manilkara
zapota
were
obtained
via
lyophilization
oven
drying.
Bromatological
analyses
performed
to
investigate
variabilities
in
nutritional
content
fruits
after
nine
post-harvest
days.
The
phytochemical
was
assessed
by
gas
chromatography
flame
ionization
detector
(GC-FID),
their
biological
performance
studied
using
vitro
antibacterial
antioxidant
assays
(DPPH
ABTS)
vivo
toxicity
models.
Molecular
docking
implemented
evaluate
interaction
between
polar
compounds
chicozapote
with
receptors
involved
pathogenesis
bacterial
strains.
Results
revealed
that
water
or
soluble
solids
did
not
vary
post-harvest.
It
demonstrated
oven-drying
approaches
influenced
insoluble,
total
dietary
fiber
digestible
carbohydrates
among
samples.
According
GC-FID
analysis,
it
observed
methods
also
altered
myristic
pentadecanoic
acids
extracts.
noted
activities
weak
due
MIC
(>1000
μg/mL)
IC50
(>2000
values.
Still,
poor
against
Artemia
salina
nauplii.
silico
evaluation
unveiled
M.
possess
a
high
binding
affinity
towards
DNA
gyrase
B
cultured
This
study
expands
scientific
evidence
regarding
influence
distinct
extraction
on
nutraceutical
native
importance
considering
additional
enhance
bioactivities.
Язык: Английский
Predictive Value of STC2 Gene Expression in Chemotherapy Response in Breast Cancer
Pharmaceuticals,
Год журнала:
2025,
Номер
18(2), С. 235 - 235
Опубликована: Фев. 8, 2025
Background:
Breast
cancer
is
the
most
commonly
diagnosed
among
women,
and
resistance
to
chemotherapy
presents
a
significant
challenge
in
its
treatment.
Stanniocalcin-2
(STC2),
glycoprotein
involved
calcium
homeostasis
cellular
stress
responses,
frequently
overexpressed
various
human
cancers.
Despite
critical
role
adaptation
stress,
potential
of
STC2
as
biomarker
for
predicting
response
has
not
been
evaluated.
This
study
aimed
assess
predictive
breast
cancer.
Methods:
We
utilized
publicly
available
databases
characterize
expression
patients
relapse-free
survival
(RFS).
Moreover,
we
evaluated
treatment
responses
subjected
chemotherapy,
correlating
their
outcomes
with
levels
determine
biomarker.
Finally,
cell
lines
following
exposure
doxorubicin
(Dox),
primary
anthracycline
used
they
were
contrasted
dataset.
Results:
The
analysis
showed
that
significantly
luminal
A
cancer,
where
it
linked
genetic
amplifications.
High
was
associated
improved
RFS
ER-positive
but
correlated
worse
ER-negative
cases.
Furthermore,
grade
II
patients,
higher
better
response,
while
poorer
response.
downregulation
observed
Dox
Conclusions:
These
findings
suggest
serves
patients.
Язык: Английский
The Emerging Roles of the Stress Epigenetic Reader LEDGF/p75 in Cancer Biology and Therapy Resistance: Mechanisms and Targeting Opportunities
Cancers,
Год журнала:
2024,
Номер
16(23), С. 3957 - 3957
Опубликована: Ноя. 26, 2024
The
lens
epithelium
derived
growth
factor
of
75
kD
(LEDGF/p75)
is
a
transcription
co-activator
and
epigenetic
reader
that
has
emerged
as
stress
oncoprotein
in
multiple
human
cancers.
Growing
evidence
indicates
it
promotes
tumor
cell
survival
against
certain
therapeutic
drugs.
amino
(N)-terminal
region
LEDGF/p75
contains
PWWP
domain
reads
methylated
histone
marks,
critical
for
recognizing
transcriptionally
active
chromatin
sites.
Its
carboxyl
(C)-terminus
an
integrase
binding
(IBD)
serves
the
site
HIV-1
oncogenic
factors.
Acting
hubs
protein-protein
interactions,
both
domains
facilitate
tethering
factors
regulators
to
regulate
mRNA
splicing,
promote
DNA
repair,
enhance
expression
cancer-related
genes
contribute
aggressiveness
chemoresistance.
This
review
summarizes
our
current
knowledge
emerging
roles
cancer
biology
therapy
resistance
discusses
its
potential
novel
oncotherapeutic
target
combinatorial
treatments.
Язык: Английский