Enhanced activation of retinal progenitor cells by zinc silicate bioceramics for effective functional restoration in retinal degenerative diseases
View,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 29, 2025
Abstract
Retinal
degenerative
diseases,
primarily
owing
to
the
gradual
loss
of
neuronal
cells,
could
lead
blindness.
Transplantation
retinal
progenitor
cells
(RPCs),
which
had
unique
ability
differentiate
into
offered
promising
therapeutic
potential
for
these
diseases.
However,
a
critical
challenge
in
stem
cell
therapy
was
efficient
promotion
directed
proliferation
and
differentiation
cells.
To
overcome
this
limitation,
we
formulated
zinc
silicate
bioceramic
(ZS)
composite
hydrogel.
The
experimental
outcomes
demonstrated
that
novel
material
possessed
substantial
advantages
immense
possibilities
mimicking
extracellular
matrix
environment,
thereby
effectively
modulating
differentiation.
Notably,
it
enhanced
RPCs
neurons
while
suppressing
glial
In
particular,
ZS
an
exceptional
capacity
induce
photoreceptor
specialized
type
cell.
It
important
highlight
hydrogel
predominantly
facilitated
through
activation
Tiam2‐Rac
signaling
pathway.
Concurrently,
stimulated
via
AKT
ERK
pathways.
Zn
ions
promoted
RPCs,
synergistic
effect
Si
contributed
reducing
inflammatory
factor
expression
promoting
RPCs.
conclusion,
meticulous
modulation
RPC
mediated
by
bioceramics
held
promise
strategies
diseases
neurodegenerative
disorders.
Язык: Английский
Identifying microglia-derived NFKBIA as a potential contributor to the pathogenesis of Alzheimer’s disease and age-related macular degeneration
Journal of Alzheimer s Disease,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
Background
Alzheimer's
disease
(AD)
and
age-related
macular
degeneration
(AMD)
place
considerable
health
burden
on
affected
individuals
significant
economic
society.
Objective
This
study
aims
to
explore
the
shared
cellular
molecular
mechanisms
underlying
pathogenesis
of
AD
AMD.
Methods
The
investigation
in
this
is
conducted
via
single-cell
bulk
tissue
transcriptomic
analysis.
Transcriptomic
datasets
AMD
were
obtained
from
GEO
database.
differentially
expressed
genes
(DEGs)
control
AD-
AMD-affected
samples
identified.
Functional
enrichment
analysis
for
DEGs
was
subsequently
performed.
Then,
protein-protein
interaction
(PPI)
network
these
established
STRING
database
hub
identified
by
Cytoscape
software.
Single-cell
performed
using
Seurat
R
package
their
expression
different
cell
types.
Results
Differential
127
two
diseases,
including
71
upregulated
56
downregulated
genes.
Upregulated
enriched
inflammation,
gliogenesis,
apoptosis,
response
bacterial
viral
infection
mitochondrial
function
energy
production.
PPI
determined
10
genes,
which
NFKBIA
gene
associated
with
severity
both
Moreover,
showed
that
highly
microglia
disease-affected
tissues.
Conclusions
findings
indicated
high
important
contributors
progression
Microglia-derived
might
serve
as
a
potential
therapeutic
target
Язык: Английский