Honeybee gut Lactobacillus modulates host learning and memory behaviors via regulating tryptophan metabolism

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Апрель 19, 2022

Honeybees are highly social insects with a rich behavioral repertoire and versatile model for neurobiological research. Their gut microbiota comprises limited number of host-restricted bacterial phylotypes that important honeybee health. However, it remains unclear how specific members affect behaviors. Here, we find antibiotic exposure disturbs the community influences phenotypes under field conditions. Using laboratory-generated gnotobiotic bees, show normal is required olfactory learning memory abilities. Brain transcriptomic profiling reveals distinct brain gene expression patterns between microbiota-free conventional bees. Subsequent metabolomic analyses both hemolymph samples mainly regulates tryptophan metabolism. Our results indicate host-specific Lactobacillus strains promote behavior by transforming to indole derivatives activate host aryl hydrocarbon receptor. findings highlight contributions neurological processes, thus providing promising understand host-microbe interactions.

Язык: Английский

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The honeybee microbiota and its impact on health and disease

Nature Reviews Microbiology, Год журнала: 2023, Номер 22(3), С. 122 - 137

Опубликована: Дек. 4, 2023

Язык: Английский

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The gut microbiota affects the social network of honeybees

Nature Ecology & Evolution, Год журнала: 2022, Номер 6(10), С. 1471 - 1479

Опубликована: Авг. 22, 2022

Язык: Английский

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Gut microbiota contribute to variations in honey bee foraging intensity

The ISME Journal, Год журнала: 2024, Номер 18(1)

Опубликована: Янв. 1, 2024

Abstract Gut microbiomes are increasingly recognized for mediating diverse biological aspects of their hosts, including complex behavioral phenotypes. Although many studies have reported that experimental disruptions to the gut microbial community result in atypical host behavior, address how microbes contribute adaptive trait variation rare. Eusocial insects represent a powerful model test this, because simple microbiota and division labor characterized by colony-level previous report correlational differences associated with labor, here, we provide evidence play causal role defining foraging behavior between European honey bees (Apis mellifera). We found structure differed hive-based nurse leave hive forage floral resources. These were abundance individual microbes, Bifidobacterium asteroides, Bombilactobacillus mellis, Lactobacillus melliventris. Manipulations colony demography experience suggested composition task experience. Moreover, single-microbe inoculations B. L. melliventris caused effects on intensity. results demonstrate social insect, support modulating variation.

Язык: Английский

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Insects' essential role in understanding and broadening animal medication

Trends in Parasitology, Год журнала: 2024, Номер 40(4), С. 338 - 349

Опубликована: Март 4, 2024

Like humans, animals use plants and other materials as medication against parasites. Recent decades have shown that the study of insects can greatly advance our understanding behaviors. The ease rearing under laboratory conditions has enabled controlled experiments to test critical hypotheses, while their spectrum reproductive strategies living arrangements – ranging from solitary eusocial communities revealed behaviors evolve maximize inclusive fitness through both direct indirect benefits. Studying also demonstrated in some cases act modulation host's innate immune system microbiome. We highlight outstanding questions, focusing on costs benefits context host fitness.

Язык: Английский

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Deep Divergence and Genomic Diversification of Gut Symbionts of Neotropical Stingless Bees

mBio, Год журнала: 2023, Номер 14(2)

Опубликована: Март 20, 2023

Social bees harbor conserved gut microbiotas that may have been acquired in a common ancestor of social and subsequently codiversified with their hosts. However, most this knowledge is based on studies the honey bumblebees. Much less known about third diverse group bees, stingless bees. Specifically, absence genomic data from presents an important gap understanding evolution functional diversity bee microbiota. Here, we combined community profiling culturing genome sequencing bacteria six neotropical species Brazil. Phylogenomic analyses show isolates form deep-branching sister clades core members bumblebee microbiota capabilities, confirming ancestry ecology our bacterial phylogenies were not congruent those host, indicating was driven by strict codiversification but included host switches independent symbiont gain losses. Finally, as reported for microbiotas, found substantial divergence among strains bacteria, suggesting adaptation to different glycan niches. Our study offers first insights into highlights need broader samplings understand IMPORTANCE Stingless are corbiculate represent pollinator throughout tropics subtropics. They specialized microbial communities related bumblebees likely health. Few cultured which has prevented characterization potential. established cultures major sequenced genomes. We belong novel distantly encoding similar capabilities. new perspective basis characterizing symbiotic relationships between

Язык: Английский

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Basic Structures of Gut Bacterial Communities in Eusocial Insects

Insects, Год журнала: 2023, Номер 14(5), С. 444 - 444

Опубликована: Май 8, 2023

Gut bacterial communities assist host animals with numerous functions such as food digestion, nutritional provision, or immunity. Some social mammals and insects are unique in that their gut microbial stable among individuals. In this review, we focus on the of eusocial insects, including bees, ants, termites, to provide an overview community structures gain insights into any general aspects structural basis. Pseudomonadota Bacillota prevalent phyla commonly detected those three insect groups, but compositions distinct at lower taxonomic levels. Eusocial harbor shared within species, while stability varies depending physiology ecology. Species narrow dietary habits, highly intraspecific communities, generalists, most ant exhibit relatively diverse structures. Caste differences could influence relative abundance members without significantly altering composition.

Язык: Английский

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Gut symbiont-derived anandamide promotes reward learning in honeybees by activating the endocannabinoid pathway

Cell Host & Microbe, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

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Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research

Insect Science, Год журнала: 2022, Номер 29(4), С. 958 - 976

Опубликована: Май 14, 2022

Abstract Eusocial bumble and honey bees are important pollinators for global ecology the agricultural economy. Although both possess similar host‐restricted gut microbiota, they differ in aspects of morphology, autonomy, physiology, behavior, life cycle. The social bee bacteria exhibit host specificity that is likely a result long‐term co‐evolution. unique cycle bumblebees key acquisition development their affects strain‐level diversity core bacterial species. Studies on bumblebee show retain less functional capacity carbohydrate metabolism compared with honeybee. We discuss potential roles microbiota against pathogenic threats application host‐specific probiotics bumblebees. Given advantages microbiome, including simple structure specificity, ease manipulating colonies, we propose may provide valuable system understanding general principles host–microbe interactions, gut–brain axis, vertical transmission.

Язык: Английский

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Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris

Frontiers in Physiology, Год журнала: 2023, Номер 13

Опубликована: Янв. 9, 2023

The sublethal effects of pesticide poisoning will have significant negative impacts on the foraging and learning bees bumblebees, so it has received widespread attention. However, little is known about physiological spinetoram glyphosate exposure bumblebees. We continuously exposed Bombus terrestris to (2.5 mg/L) or under controlled conditions for 10 days. superoxide dismutase, glutathione-S-transferase, carboxylesterase, prophenoloxidase, α-amylase protease activities, changes in gut microbes were measured understand physiology Sublethal significantly increased dismutase activity decreased bumblebees but had no effect carboxylesterase activities. In addition, prophenoloxidase. Interestingly, we observed that neither two pesticides a dominant bacteria, altered structure fungal community, reduced relative abundance Zygosaccharomyces associated with fat accumulation. These results suggest do not affect detoxification system may bumblebee health by inhibiting energy acquisition. Our provide information low concentrations terms microbes.

Язык: Английский

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