Protopine ameliorates OVA-induced asthma through modulatingTLR4/MyD88/NF-κB pathway and NLRP3 inflammasome-mediated pyroptosis

Phytomedicine, Год журнала: 2024, Номер 126, С. 155410 - 155410

Опубликована: Фев. 3, 2024

Chronic airway inflammation and hyperresponsiveness are characteristics of asthma. The isoquinoline alkaloid protopine (PRO) has been shown to exert anti-inflammatory effects, but its mechanism action in asthma is not known. Investigate the protective properties PRO upon elucidate mechanism. effects treatment were assessed by histology, biochemical analysis, real-time reverse transcription-quantitative polymerase chain reaction. Then, we integrated molecular docking, western blotting, cellular experiments, immunohistochemistry, immunofluorescence flow cytometry, metabolomics analysis reveal In vivo, therapy reduced number inflammatory cells (eosinophils, leukocytes, monocytes) bronchoalveolar lavage fluid (BALF), ameliorated pathologic alterations lung tissues, inhibited secretion lgG histamine. Molecular docking showed that could dock with proteins TLR4, MyD88, TRAF6, TAK1, IKKα, TNF-α. Western blotting displayed TLR4/NF-κB signaling pathway. regulated expression pyroptosis-related NLR family pyrin domain containing 3 (NLRP3) inflammasome, gasdermin D, caspase-1, drove caspase-1 inactivation affect responses inhibiting NLRP3 inflammasome. 24 h after PRO, cell activity, as well levels reactive oxygen species (ROS) interleukin (IL)-1β IL-18, decreased significantly. Immunofluorescence staining TLR4 MyD88 vitro. nuclear translocation NF-κB p65. Twenty-one potential biomarkers serum identified using they predominantly controlled metabolism phenylalanine, tryptophan, glucose, sphingolipids. OVA-induced underlying was associated TLR4/MyD88/NF-κB pathway inflammasome-mediated pyroptosis.

Язык: Английский

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Association of breast milk-derived arachidonic acid-induced infant gut dysbiosis with the onset of atopic dermatitis

Gut, Год журнала: 2024, Номер 74(1), С. 45 - 57

Опубликована: Июль 30, 2024

The specific breast milk-derived metabolites that mediate host-microbiota interactions and contribute to the onset of atopic dermatitis (AD) remain unknown require further investigation.

Язык: Английский

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Amorphous silica nanoparticles and the human gut microbiota: a relationship with multiple implications

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 30, 2024

Abstract Amorphous silica nanoparticles (ASNP) are among the nanomaterials that produced in large quantities. ASNP have been present for a long time several fast-moving consumer products, of which imply exposure gastrointestinal tract, such as toothpastes, food additives, drug excipients, and carriers. Consolidated use experimental evidence consistently pointed to very low acute toxicity limited absorption ASNP. However, slow implies prolonged intestinal epithelium ASNP, with documented effects on permeability immune gut homeostasis. These could explain hepatic observed after oral administration animals. More recently, role microbiota these other has attracted increasing interest parallel recognition variety conditions. Although nanomaterial is particularly abundant materials endowed bactericidal activities, growing body recent data indicates ASNPs also modify microbiota. The implications recounted this contribution, along discussion more important open issues recommendations future research. Graphical

Язык: Английский

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Protective effects of Abrus cantoniensis Hance against liver injury through modulation of intestinal microbiota and liver metabolites

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 279, С. 116495 - 116495

Опубликована: Май 30, 2024

Abrus cantoniensis Hance (ACH) is an ancient Chinese medicine herb known for its therapeutic effects. This study investigated the potential protective effect of ACH against carbon tetrachloride (CCl

Язык: Английский

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Chaihu Guizhi Ganjiang Decoction attenuates nonalcoholic steatohepatitis by enhancing intestinal barrier integrity and ameliorating PPARα mediated lipotoxicity

Journal of Ethnopharmacology, Год журнала: 2024, Номер 326, С. 117841 - 117841

Опубликована: Фев. 3, 2024

Язык: Английский

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The Role of Natural Products from Herbal Medicine in TLR4 Signaling for Colorectal Cancer Treatment

Molecules, Год журнала: 2024, Номер 29(12), С. 2727 - 2727

Опубликована: Июнь 7, 2024

The toll-like receptor 4 (TLR4) signaling pathway constitutes an intricate network of protein interactions primarily involved in inflammation and cancer. This triggers intracellular cascades, modulating transcription factors that regulate gene expression related to immunity malignancy. Previous studies showed colon cancer patients with low TLR4 exhibit extended survival times the holds a significant role CRC pathogenesis. In recent years, traditional Chinese medicines (TCMs) have garnered substantial attention as alternative therapeutic modality for CRC, due their multifaceted composition ability target multiple pathways. Emerging evidence indicates specific TCM products, such andrographolide, rosmarinic acid, baicalin, etc., potential impede development through pathway. Here, we review biochemical processes natural products from TCMs affecting sheds light on treatment strategies utilizing inhibitors which contributes advancement research accelerates clinical integration into treatment.

Язык: Английский

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Multi-omics machine learning to study host-microbiome interactions in early-onset colorectal cancer

npj Precision Oncology, Год журнала: 2024, Номер 8(1)

Опубликована: Июль 17, 2024

Abstract The incidence of early-onset colorectal cancer (eoCRC) is rising, and its pathogenesis not completely understood. We hypothesized that machine learning utilizing paired tissue microbiome plasma metabolome features could uncover distinct host-microbiome associations between eoCRC average-onset CRC (aoCRC). Individuals with stages I–IV ( n = 64) were categorized as (age ≤ 50, 20) or aoCRC ≥ 60, 44). Untargeted metabolomics 16S rRNA amplicon sequencing (microbiome analysis) tumor performed. fit DIABLO (Data Integration Analysis for Biomarker Discovery using Latent variable approaches Omics studies) to construct a supervised machine-learning classifier multi-omics metabolomics) data identify unique eoCRC. A differential association network analysis was also Distinct clustering patterns emerged in multi-omic dimension reduction analysis. achieved an AUC 0.98, compared 0.61 microbiome-based classifier. Circular correlation technique highlighted several key associations. Metabolites glycerol pseudouridine (higher abundance individuals aoCRC) had negative correlations Parasutterella , Ruminococcaceae eoCRC). Cholesterol xylitol correlated negatively Erysipelatoclostridium Eubacterium showed positive Acidovorax higher Network revealed different metabolites e.g.: urea cycle microbes such Akkermansia . show can be utilized study demonstrates promising biomarker potential may offer opportunities therapeutic interventions.

Язык: Английский

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Unravelling the prognostic and operative role of intratumoural microbiota in non‐small cell lung cancer: Insights from 16S rRNA and RNA sequencing

Clinical and Translational Medicine, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 1, 2025

Abstract Background Complex interrelationships between the microbiota and cancer have been identified by several studies. However, despite delineating microbial composition in non‐small cell lung (NSCLC), key pathogenic their underlying mechanisms remain unclear. Methods We performed 16S rRNA V3–V4 amplicon transcriptome sequencing on cancerous adjacent normal tissue samples from 30 patients with NSCLC, which clinical characteristics prognosis outcomes were collected. used to dissect perform correlations, conjunction sequencing, we determined potential underpinning significant actions. Results In comparing different sample types, more pronounced beta diversity disparity squamous carcinoma (LUSC) corresponding paired tissues. Concurrently, LUSC adenocarcinoma exhibited distinct traits at genus levels. Subsequently, four phyla, five classes, nine orders, 17 families 36 genera filtered out related outcomes. Intriguingly, a protective cluster was encompassing associated delayed disease recurrence, functional analyses suggested that these predominantly exerted metabolism‐related functions. Additionally, harmful (HMC) identified, including three genera. this HMC subsequent model analyses, intratumoural potentially implicated infection, inflammation immune regulation. Crucially, genus, Peptococcus , as an independent, detrimental NSCLC prognostic factor impacted via tumour necrosis (TNF) signalling. Conclusions substantial connection Protective primarily metabolic functions, whereas mainly modulation. Furthermore, may be adverse prognoses serve biomarker for patient management screening. Key points Four found prognosis. recurrence shorter survival earlier recurrence. impacting TNF

Язык: Английский

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Alterations in fecal bacteriome virome interplay and microbiota-derived dysfunction in patients with schizophrenia

Translational Psychiatry, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 29, 2025

Rising studies have consistently reported gut bacteriome alterations in schizophrenia (SCZ). However, little is known about the role of virome on shaping SCZ. Here in, we sequenced fecal virome, bacteriome, and host peripheral metabolome 49 SCZ patients health controls (HCs). We compared bacterial community composition specific abundant bacteria HCs. Specific viruses metabolites co-occurring with differential were identified using Multiple Co-inertia Analysis (MCIA). Additionally, construct a latent serial mediation model (SMM) to investigate effect through metabolic profile. exhibited decreased β-diversity HCs, seven differentially bacteria, including Coprobacillaceae, Enterococcaceae etc. Gut Suoliviridae Rountreeviridae, co-occur these SCZ-related bacteria. found that viral-bacterial transkingdom correlations observed HCs dramatically lost The altered profile may impact microbiota-derived enriched bile acids pathway, eicosanoids others, contributing immune dysfunction inflammation. SMM suggested potential causal chains between SCZ, indicating significantly mediated by metabolites. In conclusion, findings provide comprehensive perspective microbiota pathogenesis They reveal harbor an abnormal virome-bacteriome ecology, shedding light development microbial therapeutics.

Язык: Английский

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Integrating Transcriptomics, Metabolomics, and Microbiomics to Explore the Mechanism of Action of Bran-Fried Atractylodes Lancea Rhizome Polysaccharide in Ameliorating the Enhanced Pharmacological Effects of Dextran Sodium Sulfate-Induced Colitis

Опубликована: Янв. 1, 2025

Язык: Английский

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