Trends in Microbiology, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Nachrichten aus der Chemie, Год журнала: 2024, Номер 72(7-8), С. 59 - 62
Опубликована: Июль 1, 2024
Abstract Chemische Proteinsynthese: Neue Techniken in der Durchflusschemie und selektive Ligationsmethoden ermöglichen, komplexe präzise modifizierte Peptide Proteine für biologische Anwendungen herzustellen. Funktionelle Charakterisierung: Mit Methoden aus Mikrobiologie, chemischer Biologie Biochemie untersuchen Forschende die molekulare Funktion bakterieller Enzyme des Mikrobioms decken so deren Relevanz bei Entwicklung von Darmerkrankungen auf. DNA‐Origami: Biomoleküle auf mikro‐ nanoskopischer Ebene zu soll helfen, neue Therapeutika entwickeln, herzustellen an ihren Zielort bringen. Besonders Interaktionen Proteinen miteinander mit Ligandenmolekülen sind dabei wichtig.
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 7, 2024
Abstract Genome-scale metabolic models (GEMs) are essential tools in systems and synthetic biology, enabling the mathematical simulation of pathways encoded genomes to predict phenotypes. The complexity GEMs, however, can often limit interpretation comparison their outputs. Here, we present MMINT (Metabolic Modelling Interactive Network Tool), designed facilitate exploration networks. employs GEM networks flux solutions derived from Constraint Based Analysis (e.g. Flux Balance Analysis) create interactive visualizations. This tool allows for seamless toggling source target metabolites, network decluttering, by highlighting similarities differences between states, which enhances identification mechanistic drivers We demonstrate MMINT’s capabilities using Pyrococcus furiosus GEM, showcasing its application distinguishing acetate- ethanol-producing By providing an intuitive responsive model-exploration experience, addresses need a that simplifies outputs supports discovery novel engineering strategies. is available at https://doi.org/10.6084/m9.figshare.26409328 Graphical abstract functionalities provide solution phenotypes
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 15, 2024
Abstract Molecular hydrogen (H 2 ) is among the most central, but least understood, metabolites in human gastrointestinal tract (gut). H gas produced large quantities during bacterial fermentation and consumed as an energy source by bacteria archaea. Disruption of cycling linked to disorders, infections, cancers, with used indicator gut dysfunction through breath tests. Despite this, microorganisms, pathways, enzymes mediating production remain unresolved. Here we show that a previously uncharacterised enzyme, group B [FeFe]-hydrogenase, drives fermentative gut. Analysis stool, biopsy, isolate (meta)genomes (meta)transcriptomes this hydrogenase encoded highly expressed. Through analysis 19 taxonomically diverse isolates, [FeFe]-hydrogenase produces amounts supports growth both Bacteroidetes Firmicutes. Bacteroides particularly dominate production. Biochemical spectroscopic characterisation shows purified [FeFe]-hydrogenases are catalytically active bind di-iron site. These hydrogenases enriched guts healthy individuals, significantly depleted favour other Crohn’s disease. Furthermore, metabolically flexible respiratory abundant oxidizers gut, not sulfate reducers, methanogens, acetogens thought. This combination enzymatic, cellular, ecosystem-level provides first detailed understanding reveals new links between microbiota function health.
Язык: Английский
Процитировано
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Nippon Eiyo Shokuryo Gakkaishi, Год журнала: 2024, Номер 77(4), С. 239 - 246
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0
Trends in Microbiology, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
0