Polar Biology, Год журнала: 2024, Номер 48(1)
Опубликована: Дек. 30, 2024
Язык: Английский
Molecular Phylogenetics and Evolution, Год журнала: 2024, Номер 196, С. 108086 - 108086
Опубликована: Апрель 26, 2024
Dinoflagellates are diverse and ecologically important protists characterized by many morphological molecular traits that set them apart from other eukaryotes. These features include, but not limited to, massive genomes organized using bacterially-derived histone-like proteins (HLPs) dinoflagellate viral nucleoproteins (DVNP) rather than histones, a complex history of photobiology with independent losses photosynthesis, numerous cases serial secondary tertiary plastid gains, the presence horizontally acquired bacterial rhodopsins type II RuBisCo. Elucidating how this all evolved depends on knowing phylogenetic relationships between lineages. Half these species heterotrophic, existing data is strongly biased toward photosynthetic dinoflagellates due to their amenability cultivation prevalence in culture collections. biases make it impossible interpret evolution may also affect inferences impact our understanding character evolution. Here, we address problem isolating individual cells Salish Sea single cell, culture-free transcriptomics expand for include 39 more heterotrophic taxa, resulting roughly balanced representation. Using data, performed comprehensive search involved chromatin packaging, function, photoactivity across dinoflagellates. searches reveal 1) photosynthesis was lost at least 21 times, 2) two known types HLP were around same time sequentially as previously thought; 3) multiple present dinoflagellates, times different donors; 4) kleptoplastic have nucleus-encoded genes targeted temporary plastids they derived lineages, 5) warnowiids only heterotrophs retain whole photosystem, although some photosynthesis-related electron transport widely retained heterotrophs, likely part iron-sulfur cluster pathway persists non-photosynthetic plastids.
Язык: Английский
Процитировано
8
Current Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Current Biology, Год журнала: 2024, Номер 34(8), С. 1810 - 1816.e4
Опубликована: Апрель 1, 2024
Язык: Английский
Процитировано
6
The ISME Journal, Год журнала: 2024, Номер 18(1)
Опубликована: Янв. 1, 2024
Abstract Eupelagonemids, formerly known as Deep Sea Pelagic Diplonemids I (DSPD I), are among the most abundant and diverse heterotrophic protists in deep ocean, but little else is about their ecology, evolution, or biology general. Originally recognized solely a large clade of environmental ribosomal subunit RNA gene sequences (SSU rRNA), branching with smaller sister group DSPD II, they were postulated to be diplonemids, poorly studied branch Euglenozoa. Although new diplonemids have been cultivated depth recent years, lack cultured eupelagonemids has limited data handful light micrographs, partial SSU rRNA sequences, small number genes from single amplified genomes, only formal described species, Eupelagonema oceanica. To determine exactly where this goes tree eukaryotes begin address overall absence biological information apparently ecologically important group, we conducted single-cell transcriptomics two eupelagonemid cells. A phylogeny shows that these cells represent distinct subclades within eupelagonemids, each different E. Phylogenomic analysis based on 125-gene matrix contrasts findings ecological survey diplonemid subgroup Hemistasiidae.
Язык: Английский
Процитировано
2
Microbial Physiology, Год журнала: 2024, Номер 34(1), С. 197 - 242
Опубликована: Июль 24, 2024
Dinoflagellates are a monophyletic group within the taxon Alveolata, which comprises unicellular eukaryotes. have long been studied for their organismic and morphologic diversity as well striking cellular features. They main size range of 10-100 µm, complex "cell covering", exceptionally large genomes (∼1-250 Gbp with mean 50,000 protein-encoding genes) spread over variable number highly condensed chromosomes, perform closed mitosis extranuclear spindles (dinomitosis). Photosynthetic, marine, free-living Prorocentrum cordatum is ubiquitously occurring, bloom-forming dinoflagellate, an emerging model system, particularly respect to systems biology.
Язык: Английский
Процитировано
2
Frontiers in Marine Science, Год журнала: 2024, Номер 10
Опубликована: Янв. 4, 2024
Over the past decade, molecular phylogenies have placed endoparasites of genus Euduboscquella in a distinct subclade within clade 4 Marine Alveolate (MALV) Group I. Recently, however, rRNA gene sequences become available for four novel -like species that infect dinoflagellates, with including these indicating is paraphyletic. Here, we provide morphological characterization intracellular and extracellular life-cycle stages infects Cucumeridinium coeruleum , warm water pelagic sometimes found coastal environments. We formally describe parasite, Hobagella saltata n. gen, sp., identify constellation developmental characters distinguish it, as well melo E. nucleocola both parasites from ciliates. recommend reassignment E . comb. H. comb., respectively. also propose family Hobagellidae three congeners. anticipate character set developed distinguishing will be valuable sorting other - taxa scattered across I phylogeny insight into evolutionary patterns Lastly, consider potential influence summer-fall typhoons on occurrence its host C. near-shore waters along southeastern coast Korea hypothesis regarding northward transport onshore advection parasite populations. If future research supports hypothesized mechanisms, it could help us better understand distribution changes biogeography associated ongoing global surface seawater temperature.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Фев. 7, 2024
Abstract Ochrophyta is a vast and morphologically diverse group of algae with complex plastids, including familiar taxa fundamental ecological importance (diatoms or kelp), wealth lesser-known obscure organisms. The sheer diversity ochrophytes poses challenge for reconstructing their phylogeny, major gaps in sampling an unsettled placement particular yet to be tackled. We sequenced transcriptomes from 25 strategically selected representatives used these data build the most taxonomically comprehensive ochrophyte-centered phylogenomic supermatrix date. employed combination approaches reconstruct critically evaluate relationships among ochrophytes. While generally congruent previous analyses, updated ochrophyte tree resolved position several previously uncertain placement, supported redefinition class Synchromophyceae. Our results indicated that heterotrophic plastid-lacking heliozoan Actinophrys sol not sister lineage ochrophytes, as proposed recently, but rather phylogenetically nested them. In addition, we found Picophagus flagellatus secondarily lacking all hallmark plastid genes, exhibiting mitochondrial proteins seem genetic footprints lost organelle. thus document, first time, loss two separate lineages. Altogether, our study provides new framework trait evolution demonstrates more common than thought. Issue Section: Discoveries
Язык: Английский
Процитировано
1
Microbiology and Molecular Biology Reviews, Год журнала: 2024, Номер unknown
Опубликована: Окт. 10, 2024
SUMMARY Myzozoans encompass apicomplexans and dinoflagellates that manifest diverse lifestyles in highly varied environments. They show enormous propensity to employ different metabolic programs exploit nutrient resources niches, yet, they share much core biology underlies this evolutionary success impact. This review discusses apicomplexan parasites of medical significance the traits properties with non-pathogenic myzozoans. These include versatility myzozoan plastids, which scale from fully photosynthetic organelles site very select key pathways. Pivotal innovations, such as apical complex, have allowed myzozoans shift predatory parasitic other symbiotic multiple times both dinoflagellate branches tree. Such traits, along shared mechanisms for acquisition, appear underpin prosperity their habitats. Understanding these has potential spawn new strategic interventions against medically veterinary relevant within grouping.
Язык: Английский
Процитировано
1
Molecular Phylogenetics and Evolution, Год журнала: 2024, Номер 204, С. 108239 - 108239
Опубликована: Ноя. 17, 2024
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Март 4, 2024
Abstract The marine alveolates (MALVs) are a highly diverse group of parasitic dinoflagellates, which may regulate populations wide range hosts, including other copepods and fish eggs. Knowledge on their distribution ecological role is still limited, as they difficult to study with morphological methods. In this work, we describe the taxonomic composition, seasonal- depth MALVs in Arctic Ocean west north Svalbard, based metabarcoding data from five cruises. We recovered amplicon sequence variants (ASVs) representing all major groups previously described environmental sequencing studies (Dino-Groups I-V), Dino-Groups I II being most diverse. community was structured by season, depth, water mass. epipelagic zone, composition varied strongly however there also difference between Atlantic masses winter. spring summer communities were characterized few dominating ASVs, present low proportions during winter mesopelagic samples, suggesting that proliferate under certain conditions, e.g., when specific hosts abundant. samples more similar across sampling months, harbor parasites deep-dwelling organisms, little affected season.
Язык: Английский
Процитировано
0