The Chick Chorioallantoic Membrane as a Xenograft Model for the Quantitative Analysis of Uveal Melanoma Metastasis in Multiple Organs DOI Creative Commons
Hongtao Liu, Theodora Tsimpaki, Ralitsa Anastasova

и другие.

Cells, Год журнала: 2024, Номер 13(14), С. 1169 - 1169

Опубликована: Июль 9, 2024

Uveal melanoma (UM) is the most common intraocular tumor in adults, and nearly 50% of patients develop metastatic disease with a high mortality rate. Therefore, development relevant preclinical vivo models that accurately recapitulate cascade crucial. We exploited chick embryo chorioallantoic membrane (CAM) xenograft model to quantify both experimental spontaneous metastasis by qPCR analysis. Our study found transplanted UM cells spread predominantly early liver, reflecting primary site patients. Visible signs pigmented were observed eyes, distal CAM. Lung metastases occurred rarely brain progressed more slowly. However, cell types different origins genetic profiles caused an individual spectrum organ metastases. Metastasis multiple organs, including was often associated risk factors such as proliferation rate, hyperpigmentation, epithelioid type. The severity liver related hepatic origin chromosome 8 abnormalities rather than monosomy 3 BAP1 deficiency. presented CAM may prove useful potential or test individualized therapeutic options for organs.

Язык: Английский

Non-Cutaneous Melanoma DOI
Leanne Brown, Kelly Olino

Surgical Oncology Clinics of North America, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Процитировано

1
Optimizing TIL therapy for uveal melanoma: lessons learned and unlearned from cutaneous melanoma DOI Creative Commons
Shravan Leonard‐Murali, Udai S. Kammula

Immunotherapy, Год журнала: 2025, Номер unknown, С. 1 - 9

Опубликована: Март 18, 2025

Adoptive transfer of tumor infiltrating lymphocytes (TIL-ACT) is a personalized cancer therapy that harnesses the anti-tumor activity resident T cells through ex vivo activation and expansion. This involves infusion single dose expanded TIL together with high IL-2 following preparative lymphodepleting chemotherapy. The United States Food Drug Administration approved lifileucel in 2024 as first autologous product for patients advanced cutaneous melanoma (CM), adding to list immunotherapies this highly immunogenic cancer. However, role TIL-ACT other solid tumors unclear, especially poorly cancers low mutational burden. In review, we describe historical development TIL-ACT, summarize clinical results CM, novel application metastatic uveal (UM), prototypic immunotherapy-resistant tumor. We will highlight key biologic differences between CM UM, their consequential influence on manufacturing UM-specific products, biomarkers precision UM.

Язык: Английский

Процитировано

1
Unravelling the Molecular Pathways of Feline Diffuse Iris Melanoma Progression DOI Creative Commons
David Kayes, B. Blacklock, Robert I. McGeachan

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 31, 2025

Abstract Feline diffuse iris melanoma (FDIM) is the most common primary ocular tumour in cats, with high metastatic potential. Greater intraocular invasion correlates increased mortality. No effective therapeutics exist for disease, partly due to a lack of known molecular targets associated aggressive behaviour. Here, we define transcriptomic landscape FDIM treatment-naïve cats using bulk RNA sequencing on laser capture microdissection and core biopsy specimens from formalin-fixed paraffin-embedded tissue. Samples included ‘iris melanosis’ (dysplastic melanocytes confined anterior iris; n = 7), ‘early FDIM’ (neoplastic stroma; 13), ‘late infiltration into ciliary body sclera; 13). Iris melanosis exhibited genetic overlap early FDIM, supporting its reclassification as ‘melanoma situ.’ Early showed upregulation genes linked initiation, immune recruitment, motility (e.g., STOX1, PEG3, XIAP, MCAM, VIM). Late microenvironment remodelling, evasion, apoptosis inhibition BIRC2, BIRC5, CCL2, HAVCR2), downregulation FOX1, FOXC2, SOX11. These results provide critical biomarkers disease progression, which may aid development more accurate prognostic tests targeted therapies FDIM.

Язык: Английский

Процитировано

0
Extracellular vesicles in uveal melanoma - biological roles and diagnostic value DOI Creative Commons
Ornella Urzì, Roger Olofsson Bagge, Rossella Crescitelli

и другие.

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217531 - 217531

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Recent Advancements in Cell-Based Therapies in Melanoma DOI Open Access
George Nassief, Angela Anaeme,

Karen Moussa

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9848 - 9848

Опубликована: Сен. 12, 2024

Malignant melanoma outcomes have drastically changed in recent years due to the introduction of immune checkpoint inhibitors (ICIs). However, many patients still experience intolerable side effects, therapy resistance, and disease progression on ICI therapy. Therefore, there remains a need for novel therapeutics that address this gap treatment options. Cell-based therapies gained wide attention as therapeutic option could options advanced melanoma. These work by extracting certain cell types produced human body such T-cells, modifying them based specific target, transfusing back into patient. In realm cancer therapy, cell-based utilize cells target tumor while sparing healthy cells. Recently, Food Drug Administration (FDA) has approved usage lifileucel, tumor-infiltrating lymphocyte (TIL) This came following results from C-144-01 study (NCT02360579), which demonstrated efficacy safety TILs metastatic who otherwise failed standard ICI/targeted Thus, trial well FDA approval proven viability utilizing fill with review aims provide comprehensive overview major been utilized delineating most multi-center phase II/ III clinical trials evaluate Additionally, we summary current limitations each future direction how further extrapolate these

Язык: Английский

Процитировано

1
Advances in predictive biomarkers for melanoma immunotherapy DOI Creative Commons

Wenjie Ma,

Wanlin Liu, Jingqin Zhong

и другие.

Holistic Integrative Oncology, Год журнала: 2024, Номер 3(1)

Опубликована: Окт. 11, 2024

Abstract Purpose This review primarily discusses the current research advance of predictive biomarkers for melanoma immunotherapy. The aim present is to summarize and evaluate advantages disadvantages. Methods All reference can be found through Pubmed. mainly focuses on three main directions: tumor-related factors, host tumor microenvironment. In end, there exhibits some unusual aspects forecasts future model. Results mainsteam PD-L1, TMB, gene mutations, immune cells, IDO1, LDH, tertiary lymphoid structures (TLS), HLA-DR, tumor-associated macrophages (TAMs), tumor-infiltrating lymphocytes (TILs), Extracellular vesicles (EVs). Conclusion immunotherapy divided into parts: include TLS, TAMs, TILs, EVs. A model based multiple expected become answer predicting prognosis.

Язык: Английский

Процитировано

1
Gq/G11 oncogenic mutations promote PD-L1 expression and suppress tumor immunity DOI Creative Commons
Jingyan Dong, Yue Xu,

Dawei Yu

и другие.

European Journal of Cell Biology, Год журнала: 2024, Номер 103(4), С. 151467 - 151467

Опубликована: Ноя. 14, 2024

Uveal melanoma (UM) is the predominant form of eye cancer. The genes GNAQ and GNA11, encoding Gq G11 respectively, are most frequently mutated in UM considered major drivers carcinogenesis by activating YAP. However, mechanisms which metastatic evades immune system remain poorly understood. In this study, we found that oncogenic mutations Gq/G11 promoted YAP PD-L1 expression, modifying tumor microenvironment promoting evasion UM. Consistently, levels GNAQ/GNA11 positively correlated to expression patients. Furthermore, silencing or treating with its inhibitor, Verteporfin, attenuated induced mutations, thereby enhancing T cell activation cell-mediated cytotoxicity. Collectively, study reveals a potential role on UM, new mechanism Gq/11 mutations-induced tumorigenesis, highlighting as immunotherapeutic targets suggesting Verteporfin an adjuvant for immunotherapy patients GNA11 mutations.

Язык: Английский

Процитировано

1
Tumor infiltrating lymphocytes (TILs) – pathologia, quo vadis? - A global survey DOI Creative Commons

Kristijan Skok,

Konstantin Bräutigam

Pathology - Research and Practice, Год журнала: 2024, Номер 266, С. 155775 - 155775

Опубликована: Дек. 13, 2024

Язык: Английский

Процитировано

1
Engineered Cellular Therapies for the Treatment of Thoracic Cancers DOI Open Access
Spencer M Erickson, Benjamin M. Manning, Akhilesh Kumar

и другие.

Cancers, Год журнала: 2024, Номер 17(1), С. 35 - 35

Опубликована: Дек. 26, 2024

Thoracic malignancies (lung cancers and malignant pleural mesothelioma) are prevalent worldwide associated with high morbidity mortality. Effective treatments needed for patients advanced disease. Cell therapies a promising approach to the treatment of that make use immune effector cells have ability mediate antitumor responses. In this review, we discuss prospect chimeric antigen receptor-T (CAR-T) cells, natural killer (NK) T cell receptor-engineered (TCR-T) tumor-infiltrating lymphocytes (TILs) as thoracic malignancies. CAR-T TILs proven successful in several hematologic melanoma, respectively, but outside results thus far been unsuccessful most other solid tumors. NK TCR-T additional therapy platforms their own unique advantages challenges. Obstacles must be overcome develop effective these include selecting an appropriate target antigen, combating immunosuppressive signaling molecules present tumor microenvironment, persistence, delivering sufficient quantity tumor. Induced pluripotent stem (iPSCs) offer great promise source both cell-based due unlimited expansion potential. Here, review clinical trial data, well recent basic scientific advances insight into how may obstacles, provide overview ongoing trials testing novel strategies obstacles.

Язык: Английский

Процитировано

0
The Chick Chorioallantoic Membrane as a Xenograft Model for the Quantitative Analysis of Uveal Melanoma Metastasis in Multiple Organs DOI Creative Commons
Hongtao Liu, Theodora Tsimpaki, Ralitsa Anastasova

и другие.

Cells, Год журнала: 2024, Номер 13(14), С. 1169 - 1169

Опубликована: Июль 9, 2024

Uveal melanoma (UM) is the most common intraocular tumor in adults, and nearly 50% of patients develop metastatic disease with a high mortality rate. Therefore, development relevant preclinical vivo models that accurately recapitulate cascade crucial. We exploited chick embryo chorioallantoic membrane (CAM) xenograft model to quantify both experimental spontaneous metastasis by qPCR analysis. Our study found transplanted UM cells spread predominantly early liver, reflecting primary site patients. Visible signs pigmented were observed eyes, distal CAM. Lung metastases occurred rarely brain progressed more slowly. However, cell types different origins genetic profiles caused an individual spectrum organ metastases. Metastasis multiple organs, including was often associated risk factors such as proliferation rate, hyperpigmentation, epithelioid type. The severity liver related hepatic origin chromosome 8 abnormalities rather than monosomy 3 BAP1 deficiency. presented CAM may prove useful potential or test individualized therapeutic options for organs.

Язык: Английский

Процитировано

0