Non-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cells DOI Creative Commons

Frédéric Rosa,

Nicolas Dray, Sébastien Bedu

и другие.

Development, Год журнала: 2024, Номер 151(22)

Опубликована: Ноя. 15, 2024

Neural stem cells (NSCs) generate neurons over a lifetime in adult vertebrate brains. In the zebrafish pallium, NSCs persist long term through balanced fate decisions. These decisions include direct neuronal conversions, i.e. delamination and neurogenesis without division. To characterize this process, we reanalyze intravital imaging data of pallial NSCs, observe shared dynamics between committed progenitors. Searching for mechanisms predicting NSC build an NSC-specific genetic tracer Caspase3/7 activation (Cas3*/Cas7*) vivo. We show that non-apoptotic Cas3*/7* events occur are biased towards lineage termination under physiological conditions, with predominant generation single neurons. further identify transcription factor Atf3 as necessary bias. Finally, pathway is engaged by upon parenchymal lesion correlates more prone to neuron formation. results provide evidence caspase occurring link these decision conversion, which important long-term population homeostasis.

Язык: Английский

Interdisciplinary fetal-neonatal neurology training applies neural exposome perspectives to neurology principles and practice DOI Creative Commons
Mark S. Scher

Frontiers in Neurology, Год журнала: 2024, Номер 14

Опубликована: Янв. 15, 2024

An interdisciplinary fetal-neonatal neurology (FNN) program over the first 1,000 days teaches perspectives of neural exposome that are applicable across life span. This curriculum strengthens neonatal neurocritical care, pediatric, and adult training objectives. Teaching at maternal-pediatric hospital centers optimally merges reproductive, pregnancy, pediatric approaches to healthcare. Phenotype–genotype expressions health or disease pathways represent a dynamic developmental time. The science uncertainty applied FNN re-enforces importance shared clinical decisions minimize bias reduce cognitive errors. Trainees select mentoring committee participants will maximize their learning experiences. Standardized questions oral presentations monitor educational progress. Master doctoral defense preparation competitive research funding can be goals for specific individuals. principles practice offer an understanding gene–environment interactions recognizes effects reproductive on maternal-placental-fetal triad, neonate, child, adult. Pre-conception prenatal adversities potentially diminish life-course brain health. Endogenous exogenous toxic stressor interplay (TSI) alters through maladaptive neuroplasticity. Developmental disorders epilepsy primarily expressed during days. Communicable noncommunicable illnesses continue interact with express diverse neurologic lifespan, particularly critical/sensitive time periods adolescence senescence. Anomalous destructive fetal neuropathologic lesions change this developmental-aging continuum. integrated placental, neonatal, childhood, offers perspective exposome. Exosome promises improved monitoring drug delivery starting pregnancy. origins anticipate diagnoses interventions benefit successive generations. Addressing care disparities in Global South high-income country medical deserts require constructive dialogue among stakeholders achieve equity. Population policies capital strategy reduces global burden diseases by applying practice. integrative approach prolong survival quality persons lifespan confronted neurological disorders.

Язык: Английский

Процитировано

22
Comparative insight into the regenerative mechanisms of the adult brain in zebrafish and mouse: highlighting the importance of the immune system and inflammation in successful regeneration DOI Creative Commons
Jincan Chen, Héctor Sánchez-Iranzo, Nicolas Diotel

и другие.

FEBS Journal, Год журнала: 2024, Номер 291(19), С. 4193 - 4205

Опубликована: Авг. 6, 2024

Regeneration, the complex process of restoring damaged or absent cells, tissues, and organs, varies considerably between species. The zebrafish is a remarkable model organism for its impressive regenerative abilities, particularly in organs such as heart, fin, retina, spinal cord, brain. Unlike mammals, can regenerate with limited scarring, phenomenon closely linked to activation stem cells immune cells. This review examines unique roles played by response inflammation mouse during regeneration, highlighting cellular molecular mechanisms behind their divergent capacities. By focusing on telencephalic regeneration comparing it that rodents, this highlights importance well‐controlled, acute, non‐persistent zebrafish, which promotes an environment conducive regeneration. knowledge gained from understanding holds great promises treatment human neurodegenerative diseases brain damage (stroke traumatic injuries), well advancement medicine approaches.

Язык: Английский

Процитировано

7
Multifaceted conserved functions of Notch during post-embryonic neurogenesis in the annelidPlatynereis DOI Creative Commons
Loïc Bideau,

Loeiza Baduel,

Gabriel Krasovec

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

ABSTRACT Notch signaling is an evolutionarily conserved pathway known to orchestrate neurogenesis by regulating the transition of progenitor cells differentiated neurons and glia, as well directing neurite outgrowth axon guidance in many species. Although extensively studied vertebrates ecdysozoans, role spiralians (including mollusks, annelids or flatworms) remains largely unexplored, limiting our understanding its functions across bilaterians. In this study, we focus on segmented annelid Platynereis dumerilii , a model organism neurobiology regeneration, investigate during post-embryonic developmental processes. We show that components are expressed neurogenic territories both posterior elongation two processes requiring sustained neurogenesis. Through chemical inhibitions RNA-seq profiling, demonstrate regulates neural specification, differentiation, overall balance regenerating elongating part. Moreover, disruption activity leads severe defects pygidial central nervous system organization, including abnormal impaired outgrowth. Altogether, results support hypothesis has multifaceted bilaterians, shedding light ancestral critical pathway.

Язык: Английский

Процитировано

0
Age-dependent glial heterogeneity and traumatic injury responses in a vertebrate brain structure DOI Creative Commons

Huiwen Qin,

Shuguang Yu,

Ruyi Han

и другие.

Cell Reports, Год журнала: 2025, Номер 44(4), С. 115508 - 115508

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Prosaposin maintains adult neural stem cells in a state associated with deep quiescence DOI Creative Commons
Miriam Labusch, Melina Thétiot, Emmanuel Than‐Trong

и другие.

Stem Cell Reports, Год журнала: 2024, Номер 19(4), С. 515 - 528

Опубликована: Март 21, 2024

In most vertebrates, adult neural stem cells (NSCs) continuously give rise to neurons in discrete brain regions. A critical process for maintaining NSC pools over long periods of time the is quiescence, a reversible and tightly regulated state cell-cycle arrest. Recently, lysosomes were identified regulate quiescence-proliferation balance. However, it remains controversial whether lysosomal activity promotes proliferation or finer influence on quiescence duration depth unexplored. Using RNA sequencing pharmacological manipulations, we show that are necessary maintenance. addition, reveal expression psap, encoding regulator Prosaposin, enriched quiescent NSCs (qNSCs) reside upstream lineage display deep/long phase zebrafish telencephalon. We shRNA-mediated psap knockdown increases proportion activated (aNSCs) as well shallower states (signed by ascl1a deltaA expression). Collectively, our results identify protein Psap (direct indirect) unfold interplay between function heterogeneities.

Язык: Английский

Процитировано

2
Neural Stem Cell Regulation in Zebrafish DOI

Tanya Foley,

Melina Thétiot, Laure Bally‐Cuif

и другие.

Annual Review of Genetics, Год журнала: 2024, Номер 58(1), С. 249 - 272

Опубликована: Авг. 9, 2024

Neural stem cells (NSCs) are progenitor cell populations generating glial and neurons endowed with long-lasting self-renewal differentiation potential. While some neural progenitors (NPs) in the embryonic nervous system also long-lived match this definition, term NSC classically refers to such types adult. With discovery of extensive adult brain Danio rerio (zebrafish) their high neurogenic activity, including for neuronal regeneration, model organism has become a powerful tool characterize mechanistically dissect properties. On these bases, article will consider NSCs zebrafish brain, focus on its most extensively characterized domain, telencephalon (notably dorsal part, pallium). Whenever necessary, we refer other subdivisions, processes, mouse whether comparative purposes or because more information is available systems.

Язык: Английский

Процитировано

2
Mapping the cellular expression patterns of vascular endothelial growth factor aa and bb genes and their receptors in the adult zebrafish brain during constitutive and regenerative neurogenesis DOI Creative Commons

Danielle Fernezelian,

Sabrina Pfitsch,

Sepand Rastegar

и другие.

Neural Development, Год журнала: 2024, Номер 19(1)

Опубликована: Сен. 12, 2024

The complex interplay between vascular signaling and neurogenesis in the adult brain remains a subject of intense research. By exploiting unique advantages zebrafish model, particular persistent activity neural stem cells (NSCs) remarkable ability to repair lesions, we investigated links NSCs cerebral blood vessels. In this study, first examined gene expression profiles endothelial growth factors aa bb (vegfaa vegfbb), under physiological regenerative conditions. Employing fluorescence situ hybridization combined with immunostaining histology techniques, demonstrated widespread vegfaa vegfbb across brain, showed their presence neurons, microglia/immune cells, NSCs. At 1 day post-lesion (dpl), both were up-regulated neurons microglia/peripheral immune (macrophages). Analysis vegf receptors (vegfr) revealed high throughout homeostatic conditions, vegfr predominantly expressed lower extent cells. These findings further validated by Vegfr3 Vegfr4 immunostainings, which significant neurogenic radial glial cells.Following lesion (1 dpl), while remained stable, transcripts detected proliferative within injured parenchyma. Collectively, our results provide overview Vegf/Vegfr suggest important roles for Vegf processes.

Язык: Английский

Процитировано

1
Generating neurons in the embryonic and adult brain: compared principles and mechanisms DOI Creative Commons

Mathilde Chouly,

Laure Bally‐Cuif

Comptes Rendus Biologies, Год журнала: 2024, Номер 347(G1), С. 199 - 221

Опубликована: Ноя. 13, 2024

Neurogenesis is a lifelong process, generating neurons in the right amount, time and place with correct identity to permit growth, function, plasticity repair of nervous system, notably brain. originates from neural progenitor cells (NPs), endowed capacity divide, renew maintain population, or commit engage neurogenesis process. In adult brain, these progenitors are classically called stem (NSCs). We review here commonalities differences between NPs NSCs, their cellular molecular attributes but also potential, regulators lineage, embryonic brains. Our comparison based on two most studied model systems, namely telencephalon zebrafish mouse. discuss how population gives rise outstanding questions pertaining this transition. La neurogenèse est un processus continu qui génère des neurones en quantité, temps et lieu voulus avec l'identité correcte pour permettre la croissance, fonction, plasticité réparation du système nerveux, notamment cerveau. origine progéniteurs neuraux (PN), dotés de capacité se diviser, renouveler maintenir progénitrice, ou s'engager dans le neurogenèse. Dans cerveau adulte, ces sont classiquement appelés cellules souches neurales (CSN). Nous examinons ici les points communs différences entre PN CSN, leurs attributs cellulaires moléculaires, mais aussi leur potentiel, régulateurs lignage, cerveaux embryonnaires adultes. Notre comparaison basée sur deux systèmes modèles plus étudiés, à savoir télencéphale poisson-zèbre souris. discutons également façon dont donne naissance aux CSN adultes, restent suspens concernant cette

Процитировано

1
Fishing Innate Immune System Properties through the Transcriptomic Single-Cell Data of Teleostei DOI Creative Commons
Aleksandr Bobrovskikh, У. С. Зубаирова,

Alexey Doroshkov

и другие.

Biology, Год журнала: 2023, Номер 12(12), С. 1516 - 1516

Опубликована: Дек. 12, 2023

The innate immune system is the first line of defense in multicellular organisms.

Язык: Английский

Процитировано

1
Single-cell and in situ spatial analyses reveal the diversity of newly born hematopoietic stem cells and of their niches DOI Creative Commons
Léa Torcq,

Catherine Vivier,

Sandrine Schmutz

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 15, 2024

Abstract Hematopoietic stem cells (HSCs) and more committed progenitors (collectively referred to as HSPCs) emerge from vessels during development, via Endothelial-to-Hematopoietic Transition (EHT). Recently, using the zebrafish embryo, we showed that two EHT cell types aorta, raising question of their subsequent fate. To address this issue, established a complex pipeline based on single-cell photoconversion transgenic lines characterize transcriptomic profiles single type progenies. We obtained, at unprecedented resolution in early larva, cartography HSPCs highly diversified differentiated populations, notably NK-like types, innate lymphoid eosinophils. show previously characterized indeed lead differentially fated cells, with significant differences thymus colonization T-lymphoid lineage commitment. Using HSPC signatures retrieved our datasets – namely gata2b cd34/podocalyxin -, niches, performed situ gene expression analyses RNAscope. Unexpectedly, unveil niche contacting supra-intestinal artery. Finally, integration previous reveal populations contain potential developmental HSCs bearing similar adult HSCs. Summary Statement Single emerging hematopoietic precursor transcriptomics unravel diversity progenitor homing niches toto , development.

Язык: Английский

Процитировано

0