Fecal virus-like particles are sufficient to reduce necrotizing enterocolitis

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Авг. 22, 2024

Fecal filtrate transfer (FFT) is emerging as a safer alternative to traditional fecal microbiota transplantation (FMT) – particularly in the context of necrotizing enterocolitis (NEC), severe gastrointestinal condition affecting preterm infants. Using piglet model, FFT has demonstrated superiority over FMT safety and NEC prevention. Since virtually devoid bacteria, prokaryotic viruses (bacteriophages) are assumed mediate beneficial effects. However, this assumption remains unproven. To address gap, we separated virus-like particles (30 kDa 0.45 µm) donor feces from residual postbiotic fluid. We then compared clinical gut responses these fractions with parent solution after transferring them NEC-susceptible piglets. Virome was equally effective reducing severity NEC-like pathology. The bacterial compositional data corroborated findings virome reduced relative abundance several NEC-associated pathogens e.g. Klebsiella pneumoniae Clostridium perfringens. diversified viral communities concomitant constraining effects on composition. Unexpectedly, transfer, but not fluid, led earlier diarrhea. While diarrhea may be minor concern human infants, future work should identify ways eliminating side effect without losing treatment efficacy.

Язык: Английский

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The Gut Virome and Human Health: From Diversity to Personalized Medicine

Engineering Microbiology, Год журнала: 2025, Номер unknown, С. 100191 - 100191

Опубликована: Фев. 1, 2025

Язык: Английский

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Fecal microbiota transplantation: application scenarios, efficacy prediction, and factors impacting donor-recipient interplay

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 25, 2025

Fecal microbiota transplantation (FMT) represents a therapeutic approach that directly regulates the gut of recipients, normalizes its composition and reaping rewards. Currently, in addition to general application treating Clostridium difficile ( C. ) infection (CDI), FMT treatment has also been extended fields other gastrointestinal diseases, infections, gut-liver or gut-brain axis disorders, metabolic diseases cancer, etc. Prior FMT, rigorous donor screening is essential reduce occurrence adverse events. In addition, it imperative evaluate whether recipient can safely effectively undergo treatment. However, efficacy influenced by complex interactions between recipient, degree engraftment not necessarily positively related with success rate FMT. Furthermore, an increasing number novel factors affecting outcomes are being identified recent clinical trials animal experiments, broadening our understanding This article provides comprehensive review scenarios influencing safety from aspects both donors summarizes how these emerging regulatory be combined predict patients undergoing

Язык: Английский

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Effects of bacteriophages on gut microbiome functionality

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Март 31, 2025

The gut microbiome, composed of bacteria, fungi, and viruses, plays a crucial role in maintaining the delicate balance human health. Emerging evidence suggests that microbiome disruptions can have far-reaching implications, ranging from development inflammatory diseases cancer to metabolic disorders. Bacteriophages, or "phages", are viruses specifically infect bacterial cells, their interactions with receiving increased attention. Despite recently revived interest phageome, it is still considered "dark matter" gut, more than 80% viral genomes remaining uncharacterized. Today, research focused on understanding mechanisms by which phages influence microbiota potential applications. Bacteriophages may regulate relative abundance communities, affect functions various ways, modulate mammalian host immunity. This review explores how functionality, particularly commensals pathogens, emphasizing health disease.

Язык: Английский

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The human phageome: niche-specific distribution of bacteriophages and their clinical implications

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

ABSTRACT Bacteriophages (phages) play a crucial role in shaping the composition and diversity of human microbiome across various body niches. Recent advancements high-throughput sequencing technologies have enabled comprehensive analysis phageome different sites. This review comprehensively analyzes phage populations major niches, examining their distribution dynamics through recent metagenomic discoveries. We explore how phage–bacteria interactions within sites contribute to homeostasis disease development. Emerging evidence demonstrates that perturbations can serve as early indicators disorders, particularly gut microbiome. Understanding these complex microbial offers promising opportunities for developing novel diagnostic markers therapeutic approaches. However, causal relationship between phages, bacteria, development remains unclear. Further research is needed elucidate phages health potential or tools. intricate host unraveling complexities its impact on disease.

Язык: Английский

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Modulating intestinal viruses: A potential avenue for improving metabolic diseases with unresolved challenges

Life Sciences, Год журнала: 2024, Номер 361, С. 123309 - 123309

Опубликована: Дек. 12, 2024

Язык: Английский

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