Neuronal wiring diagram of an adult brain

Nature, Год журнала: 2024, Номер 634(8032), С. 124 - 138

Опубликована: Окт. 2, 2024

Язык: Английский

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PKA restricts ERK signaling in learning and memory Kenyon cell neurons

Cellular Signalling, Год журнала: 2025, Номер 132, С. 111818 - 111818

Опубликована: Апрель 17, 2025

Protein Kinase A (PKA) and Extracellular Signal-Regulated (ERK) have core roles in learning memory. Here, we investigate kinase-kinase signaling interactions the Drosophila brain Kenyon cell learning/memory circuit using separation of phases-based activity reporter kinase (SPARK) biosensors to image circuit-localized functions vivo. We find that constitutively active Rapidly Accelerated Fibrosarcoma (RAFgof) enhances ERK only domains with low baseline PKA signaling, transgenic inhibition function elevates signaling. Conversely, loss has no impact on whereas RAFgof expands Importantly, together synergistically These findings indicate a negative PKA-ERK pathway interaction within cells. potentiating an exogenous NaChBac ion channel function, uniformly strongly increases Similarly, thermogenetic stimulation temperature-sensitive TRPA1 PKA, (NaChBac) while also inhibiting Likewise, conditional induction (TRPA1) activity-dependent Finally, mechanically-induced seizure model (bang-sensitive sesB mutant) simultaneous this acts increase Taken together, conclude limits cells memory circuit, acting restrict

Язык: Английский

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Dynamics of two distinct memory interactions during water seeking in Drosophila

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(16)

Опубликована: Апрель 17, 2025

Forming and forgetting memories shape our self-awareness help us face future challenges. Therefore, understanding how are formed different interact in the brain is important. Previous studies have shown that thirsty flies sense humidity through ionotropic receptors, which them locate water sources. Here, we showed can be trained to associate specific odors with form a memory lasts for 30 min after association. Humidity formation requires Ir93a Ir40a essential environmental sensing. Water takes precedence, leading of by activating small subset dopaminergic neurons called protocerebral anterior medial (PAM)-γ4, project restricted region mushroom body (MB) γ lobes. Adult-stage-specific silencing Dop2R receptors MB prolongs 3 h. Live-brain calcium imaging dopamine sensor revealed significantly increased PAM-γ4 neural activity odor/humidity association, suggesting its role memory. Our results suggest overlapping circuits responsible acquisition flies.

Язык: Английский

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On analogies in vertebrate and insect visual systems

Nature reviews. Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Май 23, 2025

Язык: Английский

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Infrequent strong connections constrain connectomic predictions of neuronal function

Cell, Год журнала: 2025, Номер unknown

Опубликована: Июнь 1, 2025

Язык: Английский

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Spatial constraints and cell surface molecule depletion structure a randomly connected learning circuit

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 21, 2024

The brain can represent almost limitless objects to "categorize an unlabeled world" (Edelman, 1989). This feat is supported by expansion layer circuit architectures, in which neurons carrying information about discrete sensory channels make combinatorial connections onto much larger postsynaptic populations. Combinatorial layers are modeled as randomized sets. extent wiring exists vivo debated, and how connectivity patterns generated during development not understood. Non-deterministic algorithms could program such using minimal genomic information. Here, we investigate anatomic transcriptional perturb partner availability ask Kenyon cells, the of insect mushroom body, obtain input from olfactory projection neurons. Olfactory form their presynaptic outputs orderly, predictable, biased fashion. We find that cells accept spatially co-located but molecularly heterogeneous inputs this orderly map, cell surface molecule expression impacts choice. Cell immunoglobulins broadly depleted propose allows them with partners. model explain developmentally identical acquire diverse identities.

Язык: Английский

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The Oviposition Inhibitory Neuron is a potential hub of multi-circuit integration in the Drosophila brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 26, 2024

Abstract Understanding how neural circuits integrate sensory and state information to support context-dependent behavior is a central issue in neuroscience. In Drosophila, oviposition complex process which the fly integrates context choose an optimal location lay her eggs. The circuit that controls sequence known, but multiple modalities internal states not. We investigated circuitry underlying high-level processing related using Hemibrain connectome. identified Oviposition Inhibitory Neuron (oviIN) as key hub analyzed its inputs uncover potential parallel pathways may be responsible for computations decision-making. applied graph-theoretic analyses on sub-connectome of oviIN identify modules neurons constitute novel circuits. Our findings indicate form from unstructured neuropils Superior Protocerebrum where have been known occur.

Язык: Английский

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Disentangling how the brain is wired

Fly, Год журнала: 2024, Номер 19(1)

Опубликована: Дек. 31, 2024

Язык: Английский

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