Metagenomic insights into microbial community structure and metabolism in alpine permafrost on the Tibetan Plateau

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 14, 2024

Permafrost, characterized by its frozen soil, serves as a unique habitat for diverse microorganisms. Understanding these microbial communities is crucial predicting the response of permafrost ecosystems to climate change. However, large-scale evidence regarding stratigraphic variations in profiles remains limited. Here, we analyze community structure and functional potential based on 16S rRNA gene amplicon sequencing metagenomic data obtained from an ∼1000 km transect Tibetan Plateau. We find that alpha diversity declines but beta increases down soil profile. Microbial assemblages are primarily governed dispersal limitation drift, with importance drift decreasing increasing depth. Moreover, genes related reduction reactions (e.g., ferric iron reduction, dissimilatory nitrate denitrification) enriched subsurface layers. In addition, groups involved alternative electron accepting processes more contribute highly community-level metabolic layers, likely reflecting lower redox complicated trophic strategies microorganisms deeper soils. Overall, findings provide comprehensive insights into potentials regions.

Язык: Английский

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Dual roles of microbes in mediating soil carbon dynamics in response to warming

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 31, 2024

Abstract Understanding the alterations in soil microbial communities response to climate warming and their controls over carbon (C) processes is crucial for projecting permafrost C-climate feedback. However, previous studies have mainly focused on microorganism-mediated C release, little known about whether how affects anabolism subsequent input regions. Here, based a more than half-decade of situ experiment, we show that compared with ambient control, significantly reduces use efficiency enhances network complexity, which promotes heterotrophic respiration. Meanwhile, necromass markedly accumulates under likely due preferential decomposition plant-derived C, further leading increase mineral-associated organic C. Altogether, these results demonstrate dual roles microbes affecting release stabilization, implying feedback would weaken time dampened respiration increased proportion stable pool.

Язык: Английский

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Challenges in alpine meadow recovery: The minor effect of grass restoration on microbial resource limitation

Journal of Environmental Management, Год журнала: 2025, Номер 374, С. 124086 - 124086

Опубликована: Янв. 9, 2025

Язык: Английский

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Accelerating electron transfer reduces CH4 and CO2 emissions in paddy soil

Journal of Environmental Management, Год журнала: 2025, Номер 374, С. 124044 - 124044

Опубликована: Янв. 10, 2025

Язык: Английский

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Modeling bacterial interactions uncovers the importance of outliers in the coastal lignin-degrading consortium

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 14, 2025

Язык: Английский

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Comparative metagenomics reveals the metabolic flexibility of coastal prokaryotic microbiomes contributing to lignin degradation

Biotechnology for Biofuels and Bioproducts, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 18, 2025

Coastal wetlands are rich in terrestrial organic carbon. Recent studies suggest that microbial consortia play a role lignin degradation coastal wetlands, where turnover rates likely underestimated. However, the metabolic potentials of these remain elusive. This greatly hinders our understanding global carbon cycle and "bottom-up" design synthetic to enhance conversion. Here, we developed two groups degrading consortia, L6 L18, through 6- 18-month situ enrichments East China Sea, respectively. Lignin by L18 was 3.6-fold higher than L6. Using read-based analysis, 16S rRNA amplicon metagenomic sequencing suggested possessed varied taxonomic compositions, yet similar functional traits. Further comparative based on assembly, revealed harbored abundant metagenome-assembled genomes (MAGs) encoded diverse unique gene clusters (LDGCs). Importantly, anaerobic MAGs were significantly enriched highlighting degradation. Furthermore, generalist taxa, which possess flexibility, increased during extended enrichment period, indicating advantage generalists adapting heterogenous resources. study advances strategies prokaryotic lays foundation for communities sustainable lignocellulose biorefining.

Язык: Английский

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Leguminous and oil contaminated soils indigenous bacteria industrially more potent than unplanted soil

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

Leguminous and oil contaminated soils are considered an important rich reservoir for industrial environmentally bacteria. However, how indigenous bacteria from these different in terms of multiple enzymatic potential comparison bacterial isolates unplanted soil is needed to bio-prospect it. Here, a comparative study was conducted measure simultaneous (amylase, lipase, protease) three Bacillus spp (Bacillus sp.1, sp.2, sp.3) isolated contaminated, leguminous soils. A significant (P < 0.05) differences enzyme production among species collected types (oil leguminous, unplanted) revealed by utilizing variety substrates (starch, soybean), (olive oil, sunflower oil) (casein, soybean) respectively. Maximum amylase (21.554 ± 1.120 mm) on starch protease (14.350 1.100mm) casein observed sp.2 (leguminous soil), while lipase (20.241 1.990mm) high sp.1 soil) olive oil. sp.3 (unplanted exhibited least enzymes capabilities (3.458 O.135mm), (2.208 0.215) (4.516 0.225mm), at 37°C temperature, pH 7 after 48 hour Furthermore, significantly decreased with the increase temperature 47°C, 57°C. Results showed positive relationship between (oil-contaminated, as compare soil. These findings demonstrated that above types, have enzymes, further indicating, highly demanding habitats investigation diversity potential.

Язык: Английский

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Different effects of bio/non-degradable microplastics on sewage sludge compost performance: Focusing on antibiotic resistance genes, virulence factors and key metabolic functions

Journal of Hazardous Materials, Год журнала: 2025, Номер 488, С. 137329 - 137329

Опубликована: Янв. 27, 2025

Язык: Английский

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New insights into the assembly processes of biofilm microbiota communities: Taking the world's largest water diversion canal as a case study

The Science of The Total Environment, Год журнала: 2025, Номер 968, С. 178827 - 178827

Опубликована: Фев. 19, 2025

Язык: Английский

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Establishment and Experimental Validation of a Temperature–Unfrozen Water Content Model for Frozen Soil

Water, Год журнала: 2025, Номер 17(6), С. 846 - 846

Опубликована: Март 16, 2025

In the context of global climate change, changes in unfrozen water content permafrost significantly impact regional terrestrial plant ecology and engineering stability. Through Differential Scanning Calorimetry (DSC) experiments, this study analyzed thermal characteristic indicators, including supercooling temperature, freezing thawing critical phase-transition temperature ranges, for silt loam with varying starting moisture levels throughout cycles. With cycles, a model describing connection between soil variations during freeze–thaw cycles was established corroborated experimental data. The findings suggest that while freezing, temperatures unsaturated clay increased soil’s level, those saturated were less affected by content. During thawing, initial generally below 0 °C, exhibited power function relationship total Model analysis revealed hysteresis effects curve Both range parameters sensitive to changes, indicating processes are mainly controlled ambient changes. highlights stability difference freezing. These results offer conceptual framework comprehending mechanisms analyzing mechanical properties ecosystems caused temperature-dependent permafrost.

Язык: Английский

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