Advances in Deep Brain Imaging with Quantum Dots: Structural, Functional, and Disease-Specific Roles DOI Creative Commons

Tenesha Connor,

Hemal Weerasinghe, Justin D. Lathia

и другие.

Photonics, Год журнала: 2024, Номер 12(1), С. 3 - 3

Опубликована: Дек. 24, 2024

Quantum dots (QDs) have emerged as promising tools in advancing multiphoton microscopy (MPM) for deep brain imaging, addressing long-standing challenges resolution, penetration depth, and light–tissue interactions. MPM, which relies on nonlinear photon absorption, enables fluorescence imaging within defined volumes, effectively reducing background noise photobleaching. However, achieving greater depths remains limited by light scattering compounded the need balanced laser power to avoid tissue damage. QDs, nanoscale semiconductor particles with unique optical properties, offer substantial advantages over traditional fluorophores, including high quantum yields, large absorption cross-sections, superior photostability, tunable emission spectra. These properties enhance signal ratio at increased reduce effects, making QDs ideal subcortical regions like hippocampus without extensive microscope modifications. Studies demonstrated capability of achieve up 2100 μm, far exceeding that conventional fluorophores. Beyond structural facilitate functional applications, such high-resolution tracking hemodynamic responses neural activity, supporting investigations neuronal dynamics blood flow vivo. Their stability long-term, targeted drug delivery photodynamic therapy, presenting potential therapeutic applications treating tumors, Alzheimer’s disease, traumatic injury. This review highlights impact their effectiveness overcoming attenuation tissue, expanding role diagnosing neurological disorders, positioning them transformative agents both intervention.

Язык: Английский

Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing DOI Creative Commons
Svenja Kristin Tetzlaff, Ekin Reyhan, Nikolas Layer

и другие.

Cell, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Glioblastomas are invasive brain tumors with high therapeutic resistance. Neuron-to-glioma synapses have been shown to promote glioblastoma progression. However, a characterization of tumor-connected neurons has hampered by lack technologies. Here, we adapted retrograde tracing using rabies viruses investigate and manipulate neuron-tumor networks. Glioblastoma rapidly integrated into neural circuits across the brain, engaging in widespread functional communication, cholinergic driving invasion. We uncovered patient-specific tumor-cell-state-dependent differences synaptogenic gene expression associated connectivity subsequent invasiveness. Importantly, radiotherapy enhanced increased neuronal activity. In turn, simultaneous activity inhibition showed effects, indicative role for neuron-to-glioma contributing Lastly, rabies-mediated genetic ablation halted progression, offering viral strategy tackle glioblastoma. Together, this study provides framework comprehensively characterize networks target

Язык: Английский

Процитировано

9
Assessment of Tumor Cell Invasion and Radiotherapy Response in Experimental Glioma by Magnetic Resonance Elastography DOI Creative Commons
Hannah Fels‐Palesandro,

Sophie Heuer,

Berin Boztepe

и другие.

Journal of Magnetic Resonance Imaging, Год журнала: 2024, Номер unknown

Опубликована: Авг. 23, 2024

Background Gliomas are highly invasive brain neoplasms. MRI is the most important tool to diagnose and monitor glioma but has shortcomings. In particular, assessment of tumor cell invasion insufficient. This a clinical dilemma, as recurrence can arise from MRI‐occult invasion. Hypothesis Tumor invasion, growth radiotherapy alter parenchymal microstructure thus assessable by diffusion tensor imaging (DTI) MR elastography (MRE). Study Type Experimental, animal model. Animal Model Twenty‐three male NMRI nude mice orthotopically implanted with S24 patient‐derived cells (experimental mice) 9 stereotactically injected 1 μL PBS (sham‐injected mice). Field Strength/Sequence 2D 3D T2‐weighted rapid acquisition refocused echoes (RARE), echo planar (EPI) DTI, multi‐slice multi‐echo (MSME) T2 relaxometry, MSME MRE at 900 Hz acquired 9.4 T (675 mT/m gradient strength). Assessment Longitudinal 4‐weekly was performed for up 4 months. volume assessed in experimental (n = 10 treatment‐control, n 13 radiotherapy). The subgroup 5 sham‐injected underwent irradiation (3 × 6 Gy) weeks post‐implantation/sham injection. MRI‐/MRE‐parameters were corpus callosum core/injection tract. Imaging data correlated light sheet microscopy (LSM) histology. Statistical Tests Paired unpaired t ‐tests, P ‐value ≤0.05 considered significant. Results From week 8, significant callosal stiffening (4.44 ± 0.22 vs. 5.31 0.29 kPa) detected correlating LSM‐proven occult all other metrics. Histologically proven tissue destruction core led an increased relaxation time (41.65 0.34 44.83 0.66 msec) ADC (610.2 12.27 711.2 13.42 −6 mm 2 /s) softening (5.51 0.30 4.24 8 12. Radiotherapy slowed progression. Data Conclusion promising key characteristics. Evidence Level NA Technical Efficacy Stage

Язык: Английский

Процитировано

5
Beyond the EPR effect: Intravital microscopy analysis of nanoparticle drug delivery to tumors DOI Creative Commons
Iaroslav B. Belyaev, Olga Yu. Griaznova, Alexey V. Yaremenko

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2025, Номер unknown, С. 115550 - 115550

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
The characteristics of capillary remodeling in cerebellar neurodegenerative diseases revealed through layered imaging and stereoscopic analysis DOI Creative Commons
Yayun Wang, Hui Liu,

Ziwei Ni

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Abstract Neurodegenerative diseases refer to a group of clinical conditions characterized by progressive neuronal loss, resulting in impaired brain structural integrity and functional abnormalities. These can lead widespread cerebrovascular remodeling; however, the spatial remodeling features capillaries with diameters ≤ 10 μm remain poorly understood, particularly regard changes relationship between neurons capillaries. In this study, we first developed double-fluorescent transgenic mouse model cerebellar neurodegenerative disease (CBND), PCKOTomatoVesselGreen mouse, which Purkinje cells (PCs) cerebellum express red fluorescent protein, while system expresses green protein (with no differentiation arteries veins). Subsequently, employed whole-brain clearing combined Amira/Imaris conduct three-dimensional layered imaging computational analysis network both adult control mice. A total 181744 PCs vessels length 17.7363 meters, 266175 segments, volume 0.5314 mm³ were analyzed. Compared Control mice, mice exhibited 93% reduction count PCs, 97% 69% volume, 58% decrease vessel length, 52% vascular volume. Depth revealed 55%, 58%, capillary chord curved tortuosity, respectively, statistical differences node or φ θ directional values. Further fraction (VF) 59% increase capillary-cerebellum VF, PC-vessel PC-capillary PC-noncapillary VF decreased 95%, 96%, respectively. Additionally, shortest distance vessel-cerebellum noncapillary-cerebellum showed differences. Our results indicated that significantly lost, their topology remained stable, decreasing from 16 7 μm. This process is central pathogenesis CBND. Furthermore, may serve as biological markers for early diagnosis findings provide foundation development targeted therapies Short abstract Cerebrovascular caused be used diagnosis, but its characteristics are unclear. research constructed disease, then adopted whole transparency system. m, number segments mm3 analyzed stratified analysis. 3.15 TB data lost although stable. blood μm, identifying feature neurovascular disease. The cell-vessel diseases.

Язык: Английский

Процитировано

0
Reconstructing the Single-Cell Spatiotemporal Dynamics of Glioblastoma Invasion DOI Creative Commons
Sven Nelander, Hitesh Bhagavanbhai Mangukiya,

Madeleine Skeppas

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Abstract Glioblastoma invasion into healthy brain tissue remains a major barrier to effective treatment, yet current models fail capture its full complexity in scalable and patient-specific manner. Here, we introduce GlioTrace, novel ex vivo imaging AI-based analytical framework that enables real-time, spatiotemporal tracking of glioblastoma dynamics patient-derived glioma cell culture xenograft (PDCX) slices. By integrating whole-specimen confocal microscopy, vascular counterstaining, an advanced computational pipeline combining convolutional neural networks Hidden Markov Models, GlioTrace identifies distinct modes—including dynamic morphological switching, vessel-guided migration, immune interactions—and quantifies variations plasticity. Using demonstrate targeted therapies can selectively modulate phenotypes, revealing spatially temporally drug responses. This platform provides unprecedented window progression treatment response, offering powerful tool for precision oncology anti-invasion therapeutic development.

Язык: Английский

Процитировано

0
Predicting glioblastoma progression using MR diffusion tensor imaging: A systematic review DOI Creative Commons
F. Cozzi,

Roxanne Mayrand,

Yizhou Wan

и другие.

Journal of Neuroimaging, Год журнала: 2024, Номер 35(1)

Опубликована: Дек. 9, 2024

Abstract Background and purpose Despite multimodal treatment of glioblastoma (GBM), recurrence beyond the initial tumor volume is inevitable. Moreover, conventional MRI has shortcomings that hinder early detection occult white matter tract infiltration by tumor, but diffusion tensor imaging (DTI) a sensitive probe for assessing microstructural changes, facilitating identification progression before standard imaging. This sensitivity makes DTI valuable tool predicting recurrence. A systematic review was therefore conducted to investigate how DTI, in comparison MRI, can be used GBM progression. Methods We queried three databases (PubMed, Web Science, Scopus) using search terms: (diffusion OR DTI) AND (glioblastoma GBM) (recurrence progression). For included studies, data pertaining study type, number patients, type(s), DTI‐related metrics were extracted. Results In all, 16 studies included, from which there 394 patients total. Six reported decreased fractional anisotropy regions, 2 described utility connectomics/tractography migratory pathways site Three evidence visible on Conclusions These findings suggest may useful guiding surgical radiotherapy planning informing long‐term surveillance. Understanding current state literature these metrics’ trends crucial, particularly as increasingly treatment‐guiding modality.

Язык: Английский

Процитировано

1
Advances in Deep Brain Imaging with Quantum Dots: Structural, Functional, and Disease-Specific Roles DOI Creative Commons

Tenesha Connor,

Hemal Weerasinghe, Justin D. Lathia

и другие.

Photonics, Год журнала: 2024, Номер 12(1), С. 3 - 3

Опубликована: Дек. 24, 2024

Quantum dots (QDs) have emerged as promising tools in advancing multiphoton microscopy (MPM) for deep brain imaging, addressing long-standing challenges resolution, penetration depth, and light–tissue interactions. MPM, which relies on nonlinear photon absorption, enables fluorescence imaging within defined volumes, effectively reducing background noise photobleaching. However, achieving greater depths remains limited by light scattering compounded the need balanced laser power to avoid tissue damage. QDs, nanoscale semiconductor particles with unique optical properties, offer substantial advantages over traditional fluorophores, including high quantum yields, large absorption cross-sections, superior photostability, tunable emission spectra. These properties enhance signal ratio at increased reduce effects, making QDs ideal subcortical regions like hippocampus without extensive microscope modifications. Studies demonstrated capability of achieve up 2100 μm, far exceeding that conventional fluorophores. Beyond structural facilitate functional applications, such high-resolution tracking hemodynamic responses neural activity, supporting investigations neuronal dynamics blood flow vivo. Their stability long-term, targeted drug delivery photodynamic therapy, presenting potential therapeutic applications treating tumors, Alzheimer’s disease, traumatic injury. This review highlights impact their effectiveness overcoming attenuation tissue, expanding role diagnosing neurological disorders, positioning them transformative agents both intervention.

Язык: Английский

Процитировано

0