Interneuron migration impairment and brain region-specific DNA damage response following irradiation during early neurogenesis in mice DOI Creative Commons
Lisa Berden, Nicholas Rajan,

André Claude Mbouombouo Mfossa

и другие.

Cellular and Molecular Life Sciences, Год журнала: 2025, Номер 82(1)

Опубликована: Март 17, 2025

Abstract Embryonic DNA damage resulting from repair deficiencies or exposure to ionizing radiation during early neurogenesis can lead neurodevelopmental disorders, including microcephaly. This has been linked an excessive response in dorsal neural progenitor cells (NPCs), p53-dependent apoptosis and premature neuronal differentiation which culminates depletion of the NPC pool. However, effect on ventral forebrain NPCs, origin interneurons, remains unclear. In this study, we investigated sequelae irradiation mouse fetuses at timepoint neurogenesis. We focused neocortex (NCX) medial ganglionic eminence (MGE), key regions for developing respectively. Although both showed a typical p53-mediated consisting cell cycle arrest, apoptosis, NCX displayed prolonged while MGE exhibited more sustained apoptosis. Moreover, reduced migration speed interneurons acute living brain slices explants, latter indicating cell-intrinsic component defect. RNA sequencing protein analyses revealed disruptions actin microtubule cytoskeletal-related cellular machinery, particularly cells. Despite massive obvious interneuron defect, prenatally irradiated animals did not show increased sensitivity pentylenetetrazole-induced seizures, nor was there reduction cortical young adult mice. suggests high plasticity insults Overall, our findings indicate that embryonic induces region-specific responses, potentially disorders.

Язык: Английский

Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress DOI Creative Commons
Martina Lo Sterzo, Domenico Iuso, Luca Palazzese

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

0
Redox-Guided DNA Scanning by the Dynamic Repair Enzyme Endonuclease III DOI Creative Commons
Ayaz Hassan, Filipe C. D. A. Lima, Frank N. Crespilho

и другие.

Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Endonuclease III (EndoIII), a key enzyme in the base excision repair (BER) pathway, contains [4Fe4S] cluster that facilitates DNA through DNA-mediated charge transfer. Recent findings indicate redox state of this influences EndoIII's binding affinity for DNA, modulating enzyme's activity. In study, we investigated structural and electronic changes upon to double-stranded (dsDNA) using Fourier transform infrared spectroscopy, density functional theory calculations, machine learning models. Our results reveal shifts Fe-S bond vibrational modes, suggesting stabilization oxidized proximity negatively charged DNA. A model, trained on spectral features EndoIII/DNA complex, predicted enzyme-DNA distance, providing further insights into binding. We correlated electrochemical potential 150 mV with DNA-binding properties, demonstrating how cluster's plays crucial role both stability repair.

Язык: Английский

Процитировано

0
Micronuclei formation: small nuclear packages with big genomic consequences DOI
Andrew Omachoko Onoja, Christopher Didigwu Nwani, Chibuisi G. Alimba

и другие.

The Nucleus, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0
Interneuron migration impairment and brain region-specific DNA damage response following irradiation during early neurogenesis in mice DOI Creative Commons
Lisa Berden, Nicholas Rajan,

André Claude Mbouombouo Mfossa

и другие.

Cellular and Molecular Life Sciences, Год журнала: 2025, Номер 82(1)

Опубликована: Март 17, 2025

Abstract Embryonic DNA damage resulting from repair deficiencies or exposure to ionizing radiation during early neurogenesis can lead neurodevelopmental disorders, including microcephaly. This has been linked an excessive response in dorsal neural progenitor cells (NPCs), p53-dependent apoptosis and premature neuronal differentiation which culminates depletion of the NPC pool. However, effect on ventral forebrain NPCs, origin interneurons, remains unclear. In this study, we investigated sequelae irradiation mouse fetuses at timepoint neurogenesis. We focused neocortex (NCX) medial ganglionic eminence (MGE), key regions for developing respectively. Although both showed a typical p53-mediated consisting cell cycle arrest, apoptosis, NCX displayed prolonged while MGE exhibited more sustained apoptosis. Moreover, reduced migration speed interneurons acute living brain slices explants, latter indicating cell-intrinsic component defect. RNA sequencing protein analyses revealed disruptions actin microtubule cytoskeletal-related cellular machinery, particularly cells. Despite massive obvious interneuron defect, prenatally irradiated animals did not show increased sensitivity pentylenetetrazole-induced seizures, nor was there reduction cortical young adult mice. suggests high plasticity insults Overall, our findings indicate that embryonic induces region-specific responses, potentially disorders.

Язык: Английский

Процитировано

0