Engineering drive–selection balance for localized population suppression with neutral dynamics DOI Creative Commons
Katie Willis, Austin Burt

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(6)

Опубликована: Фев. 4, 2025

While the release of sterile males has been highly successful in suppressing some pest populations, it is impractical for many species due to disappearing after a single generation, necessitating large, repeated releases maintain sufficient impact. Synthetic gene drives promise more efficient approaches since they can increase frequency from rare, yet this also allows them spread across landscape, which may not always be desired. Between these two extremes are selectively neutral genetic constructs persist at released, offering potential suppression that remains localized. One way achieve would have perfect balance, all construct frequencies, between drive increasing and selection decreasing it. Here, we describe closely approximate balance using toxin–antidote causes recessive lethality or sterility, encodes genomic editor makes dominant lethal edits genome, provides protection against action consequences editing. Computer modeling shows design 100-fold than males, 1,000-fold when released alongside booster. We designs CRISPR-based molecular construction, including options avoid recoded genes as antidotes.

Язык: Английский

Gene drive-based population suppression in the malaria vector Anopheles stephensi DOI Creative Commons
Xuejiao Xu,

Jingheng Chen,

You Wang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 24, 2025

Gene drives are alleles that can bias the inheritance of specific traits in target populations for purpose modification or suppression. Here, we construct a homing suppression drive major urban malaria vector Anopheles stephensi targeting female-specific exon doublesex, incorporating two gRNAs and nanos-Cas9 to reduce functional resistance improve female heterozygote fitness. Our results show was recessive sterile both females males, with various intersex phenotypes homozygotes. Both male heterozygotes only moderate conversion, indicating nanos promoter has lower activity A. than gambiae. By amplicon sequencing, detect very low level allele formation. Combination vasa-Cas9 line boosts conversion rate 100%, suggesting use similar systems population continuous release strategy SIT fsRIDL techniques. This study contributes valuable insights development more efficient environmentally friendly pest control tools aimed at disrupting disease transmission. their spread through populations. constructed efficiency 2-gRNA mosquito doublesex. With vasa-Cas9, high self-limiting achieved.

Язык: Английский

Процитировано

1
Engineering drive–selection balance for localized population suppression with neutral dynamics DOI Creative Commons
Katie Willis, Austin Burt

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(6)

Опубликована: Фев. 4, 2025

While the release of sterile males has been highly successful in suppressing some pest populations, it is impractical for many species due to disappearing after a single generation, necessitating large, repeated releases maintain sufficient impact. Synthetic gene drives promise more efficient approaches since they can increase frequency from rare, yet this also allows them spread across landscape, which may not always be desired. Between these two extremes are selectively neutral genetic constructs persist at released, offering potential suppression that remains localized. One way achieve would have perfect balance, all construct frequencies, between drive increasing and selection decreasing it. Here, we describe closely approximate balance using toxin–antidote causes recessive lethality or sterility, encodes genomic editor makes dominant lethal edits genome, provides protection against action consequences editing. Computer modeling shows design 100-fold than males, 1,000-fold when released alongside booster. We designs CRISPR-based molecular construction, including options avoid recoded genes as antidotes.

Язык: Английский

Процитировано

0