Genome‐Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells DOI Creative Commons
Xiusheng Zhu, Lei Huang,

Guoli Li

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

The majority of the mouse genome is composed non-coding regions, which harbor numerous regulatory sequences essential for gene regulation. While extensive research focuses on enhancers that activate expression, role silencers repress expression remains less explored. In this study, first genome-wide identification in conducted. embryonic fibroblasts (MEFs) and stem cells (mESCs), 89 596 115 165 are identified, respectively. These ubiquitously distributed across predominantly associated with low-expression genes. Additionally, these mainly cell-specific function by binding to repressive transcription factors (TFs). Further, notably enriched histone modification H3K9me3. It observed transformation between dual-function correlated intracellular factor concentrations, accompanied changes epigenetic modifications. terms biological effects, we have identified can enhance induction efficiency MEFs influence pluripotency mESCs. Collectively, work offers comprehensive silencer landscape provides strong evidence induced pluripotent (iPSCs).

Язык: Английский

Genome‐Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells DOI Creative Commons
Xiusheng Zhu, Lei Huang,

Guoli Li

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

The majority of the mouse genome is composed non-coding regions, which harbor numerous regulatory sequences essential for gene regulation. While extensive research focuses on enhancers that activate expression, role silencers repress expression remains less explored. In this study, first genome-wide identification in conducted. embryonic fibroblasts (MEFs) and stem cells (mESCs), 89 596 115 165 are identified, respectively. These ubiquitously distributed across predominantly associated with low-expression genes. Additionally, these mainly cell-specific function by binding to repressive transcription factors (TFs). Further, notably enriched histone modification H3K9me3. It observed transformation between dual-function correlated intracellular factor concentrations, accompanied changes epigenetic modifications. terms biological effects, we have identified can enhance induction efficiency MEFs influence pluripotency mESCs. Collectively, work offers comprehensive silencer landscape provides strong evidence induced pluripotent (iPSCs).

Язык: Английский

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