bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Сен. 24, 2024
Abstract Many bacterial habitats, from the gut to soil, feature narrow channels where confined flow is a key constraint that might influence spatial organization, and thus functioning, of complex, phenotypically heterogeneous communities these microbes form. Here, we investigate how model community motile non-motile Escherichia coli organizes under Poiseuille flow. We discovered new mode active self-organization, bacteria induce rapid sideways segregation cells one side channel, eventually resulting in asymmetric biofilm formation. Our experiments modeling elucidated purely physical mechanism: rheotactic drift cells, which stems shear acting on their chiral flagella, induces conveyer-belt-like backflow advecting cells. The latter then accumulate thanks sedimentation countering incompressibility. This unexpected consequence motility can affect organization complex colonizing environments
Язык: Английский