Decoding the complex journeys of RNAs along neurons DOI Creative Commons
Jenna L. Wingfield, Sathyanarayanan V. Puthanveettil

Nucleic Acids Research, Год журнала: 2025, Номер 53(7)

Опубликована: Апрель 10, 2025

Abstract Neurons are highly polarized, specialized cells that must overcome immense challenges to ensure the health and survival of organism in which they reside. They can spread over meters persist for decades yet communicate at sub-millisecond millimeter scales. Thus, neurons require extreme levels spatial-temporal control. employ molecular motors transport coding noncoding RNAs distal synapses. Intracellular trafficking enables locally regulate protein synthesis synaptic activity. The way get loaded onto transported their target locations, particularly following plasticity, is explored below.

Язык: Английский

EFFICIENT RIBOSOMAL RNA DEPLETION FROM DROSOPHILA TOTAL RNA FOR NEXT-GENERATION SEQUENCING APPLICATIONS DOI Creative Commons

Omkar Koppaka,

Shweta Tandon, Ankita Chodankar

и другие.

Wellcome Open Research, Год журнала: 2025, Номер 10, С. 112 - 112

Опубликована: Май 13, 2025

We developed a cost-effective enzyme-based rRNA-depletion method tailored for Drosophila melanogaster, addressing the limitations of existing commercial kits and lack peer-reviewed alternatives. Our employs single-stranded DNA probes complementary to Drosophila rRNA, forming DNA-RNA hybrids. These hybrids are then degraded using RNase H enzyme, effectively removing rRNA enriching all non-ribosomal RNAs, including mRNA, lncRNA small RNA. When compared removal kit, our approach demonstrated superior efficiency mapping percentage, confirming its effectiveness. Additionally, successfully enriched non-coding transcriptome, making it valuable tool studying ncRNA in Drosophila. The probe sequences protocol made freely available, offering reliable alternative experiments.

Язык: Английский

Процитировано

1
Decoding the complex journeys of RNAs along neurons DOI Creative Commons
Jenna L. Wingfield, Sathyanarayanan V. Puthanveettil

Nucleic Acids Research, Год журнала: 2025, Номер 53(7)

Опубликована: Апрель 10, 2025

Abstract Neurons are highly polarized, specialized cells that must overcome immense challenges to ensure the health and survival of organism in which they reside. They can spread over meters persist for decades yet communicate at sub-millisecond millimeter scales. Thus, neurons require extreme levels spatial-temporal control. employ molecular motors transport coding noncoding RNAs distal synapses. Intracellular trafficking enables locally regulate protein synthesis synaptic activity. The way get loaded onto transported their target locations, particularly following plasticity, is explored below.

Язык: Английский

Процитировано

0