Mapping RNA–Protein Interactions via Proximity Labeling‐Based Approaches DOI
Y. Chen, Yuxin Chen, Wei Qin

и другие.

Chemistry - An Asian Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

Abstract RNA–protein interactions are fundamental to a wide range of biological processes, and understanding these in their native cellular context is both vital challenging. Traditional methods for studying rely on crosslinking, which can introduce artifacts. Recently, proximity labeling‐based techniques have emerged as powerful alternatives, offering crosslinking‐free approach investigate interactions. This review highlights recent advancements the development application labeling methods, focusing RNA‐centric protein‐centric strategies profiling By examining innovative approaches, we aim provide insights into potential enhancing our dynamics various settings.

Язык: Английский

Engineered Proteins and Chemical Tools to Probe the Cell Surface Proteome DOI Creative Commons
Kevin K. Leung, Kaitlin Schaefer, Zhi Lin

и другие.

Chemical Reviews, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

The cell surface proteome, or surfaceome, is the hub for cells to interact and communicate with outside world. Many disease-associated changes are hard-wired within yet approved drugs target less than 50 proteins. In past decade, proteomics community has made significant strides in developing new technologies tailored studying surfaceome all its complexity. this review, we first dive into unique characteristics functions of emphasizing necessity specialized labeling, enrichment, proteomic approaches. An overview surfaceomics methods provided, detailing techniques measure protein expression how leads novel discovery. Next, highlight advances proximity labeling (PLP), showcasing various enzymatic photoaffinity can map protein-protein interactions membrane complexes on surface. We then review role extracellular post-translational modifications, focusing glycosylation, proteolytic remodeling, secretome. Finally, discuss identifying tumor-specific peptide MHC they have shaped therapeutic development. This emerging field neo-protein epitopes constantly evolving, where targets identified at proteome level encompass defined PTMs, complexes, dysregulated cellular tissue locations. Given functional importance biology therapy, view as a critical piece quest neo-epitope

Язык: Английский

Процитировано

0
Mapping RNA–Protein Interactions via Proximity Labeling‐Based Approaches DOI
Y. Chen, Yuxin Chen, Wei Qin

и другие.

Chemistry - An Asian Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

Abstract RNA–protein interactions are fundamental to a wide range of biological processes, and understanding these in their native cellular context is both vital challenging. Traditional methods for studying rely on crosslinking, which can introduce artifacts. Recently, proximity labeling‐based techniques have emerged as powerful alternatives, offering crosslinking‐free approach investigate interactions. This review highlights recent advancements the development application labeling methods, focusing RNA‐centric protein‐centric strategies profiling By examining innovative approaches, we aim provide insights into potential enhancing our dynamics various settings.

Язык: Английский

Процитировано

0