Community standards and future opportunities for synthetic communities in plant–microbiota research

Nature Microbiology, Год журнала: 2024, Номер 9(11), С. 2774 - 2784

Опубликована: Окт. 30, 2024

Язык: Английский

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Maize zinc uptake is influenced by arbuscular mycorrhizal symbiosis under various soil phosphorus availabilities

New Phytologist, Год журнала: 2024, Номер 243(5), С. 1936 - 1950

Опубликована: Июль 7, 2024

Summary The antagonistic interplay between phosphorus (P) and zinc (Zn) in plants is well established. However, the molecular mechanisms mediating those interactions as influenced by arbuscular mycorrhizal (AM) symbiosis remain unclear. We investigated Zn concentrations, root AM symbiosis, transcriptome profiles of maize roots grown under field conditions upon different P levels. also validated genotype‐dependent P–Zn uptake selected genotypes from a MAGIC population conducted inoculation experiments using mycorrhizal‐defective mutant pht1;6 to elucidate significance antagonism. Finally, we assessed how supply affects transporters extraradical hyphae within three‐compartment system. Elevated levels led significant reduction concentration across population, correlating with marked decline thus elucidating identified ZmPht1;6 crucial for confirmed that dependent on symbiosis. Moreover, found high suppressed expression fungal RiZRT1 RiZnT1 genes, potentially impacting hyphal uptake. conclude exerts systemic regulation over hyphae‐mediated maize. These findings hold implications breeding deficiency‐tolerant varieties.

Язык: Английский

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Exploring the potential of seed inoculation with microbial consortia to mitigate drought stress in maize plants under greenhouse conditions

Plant and Soil, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Abstract Background and aims Plant-beneficial microbes may attenuate climate change-induced stresses on plants such as drought. We investigated the potential of beneficial microbial consortia (BMc) plant growth rhizosphere bacterial/archaeal community under Methods Seeds Zea mays B73 were inoculated with six plant-beneficial bacterial isolates either alone or combined in two three-member (BMc1, BMc2) before sowing loamy sandy substrates greenhouse. A known effective consortium (BMc3) was included positive control. Drought treatment established BMc treatments by omitting watering last five weeks period. The maize single determined. Colony-forming units (CFUs) inoculants evaluated selective plating, effects native assessed using 16S rRNA gene amplicon sequencing basal root tip grown loam. Results In both water conditions, CFUs inoculations higher at roots than tips. Under well-watered seed inoculation a isolate had no effect substrates. resulted shoot (but not root) compared to non-inoculated controls conditions zone most important driver for beta-diversity, followed while showed effect. Conclusion Our study suggests that has drought stress during growth.

Язык: Английский

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Molecular concepts to explain heterosis in crops

Trends in Plant Science, Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

Heterosis describes the superior performance of hybrid plants compared with their genetically distinct parents and is a pillar global food security. Here we review current status molecular dissection heterosis. We discuss how extensive intraspecific structural genomic variation between parental genotypes leads to heterosis by genetic complementation in hybrids. Moreover, survey gene expression contributes hundreds additionally active genes hybrids overdominant single mediate several species. Furthermore, highlight prominent role microbiome improving Taken together, understanding will pave way accelerate productivity more sustainable agriculture.

Язык: Английский

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Exploitation of the microbiome for crop breeding

Nature Plants, Год журнала: 2024, Номер 10(4), С. 533 - 534

Опубликована: Март 21, 2024

Язык: Английский

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Editorial: Microbiome associated with plant pathogens, pathogenesis, and their applications in developing sustainable agriculture

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 21, 2024

Editorial: Microbiome associated with plant pathogens, pathogenesis, and their applications in developing sustainable agriculture

Язык: Английский

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Determining the footprint of breeding in the seed microbiome of a perennial cereal

Environmental Microbiome, Год журнала: 2024, Номер 19(1)

Опубликована: Июнь 17, 2024

Abstract Background Seed endophytes have a significant impact on plant health and fitness. They can be inherited passed to the next generation. However, of breeding their composition in seeds is less understood. Here, we studied indigenous seed microbiome recently domesticated perennial grain crop (Intermediate wheatgrass, Thinopyrum intermedium L.) that promises great potential for harnessing microorganisms enhance performance by multiphasic approach, including amplicon strain libraries, as well molecular physiological assays. Results Intermediate wheatgrass harvested from four field sites Europe over three consecutive years were dominated Proteobacteria (88%), followed Firmicutes (10%). Pantoea was most abundant genus agglomerans identified only core taxon present all samples. While bacterial diversity species richness similar across accessions, relative abundance varied especially terms low rare taxa. Seeds different cycles (TLI C3, C5, C704, C801) showed differences community abundance. We found decrease functional genes nirK nifH drop richness. This associated with loss sequence variants (ASVs) Actinobacteria , Alphaproteobacteria Bacilli which could partially compensated offspring seeds, been cultivated at new site. Interestingly, subset assigned potentially beneficial bacteria, e.g. Pantoea, Kosakonia Pseudomonas transmitted generation or shared seeds. Conclusion Overall, this study advances our understanding assembly transmission endophytic intermediate highlights importance considering future programs.

Язык: Английский

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Lateral root enriched Massilia associated with plant flowering in maize

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Июль 9, 2024

Abstract Background Beneficial associations between plants and soil microorganisms are critical for crop fitness resilience. However, it remains obscure how assembled across different root compartments to what extent such recruited microbiomes determine performance. Here, we surveyed the transcriptome rhizosphere microbiome via RNA sequencing full-length (V1–V9) 16S rRNA gene from genetically distinct monogenic mutants of maize ( Zea mays L.) under nutrient-limiting conditions. Results Overall display a clear assembly pattern compartments, i.e., through tissues. Co-variation analysis identified that genotype dominated effect on microbial community expression over nutrient stress Integrated transcriptomic analyses demonstrated mutations affecting lateral development had largest host assembly, as compared other types. Cooccurrence trans-kingdom network association keystone bacterial taxon Massilia Oxalobacteraceae ) is associated with functional genes involved in flowering time overall plant biomass. We further observed developmental stage drives differentiation especially bacteria reproduction. Taking advantage inoculation experiments using early mutant, confirmed -driven growth promotion indeed depends time. Conclusion conclude specific microbiota supporting formation could enhance performance by mediating underlying maize.

Язык: Английский

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From synthetic communities to synthetic ecosystems: exploring causalities in plant–microbe–environment interactions

New Phytologist, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Summary The plant microbiota research field has rapidly shifted from efforts aimed at gaining a descriptive understanding of composition to focus on acquiring mechanistic insights into functions and assembly rules. This evolution was driven by our ability establish comprehensive collections plant‐associated microbes reconstruct meaningful microbial synthetic communities (SynComs). We argue that this powerful deconstruction–reconstruction strategy can be used reconstitute increasingly complex ecosystems (SynEcos) mechanistically understand high‐level biological organization. transitioning simple more advanced, fully tractable programmable gnotobiotic SynEcos is ongoing aims rationally simplifying natural engineering them. Such reconstitution ecology approaches represent an untapped for bridging the gap between functional biology unraveling plant–microbiota–environment mechanisms modulate ecosystem health, assembly, functioning.

Язык: Английский

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Sustainable soil management in agriculture under drought stress: Utilising waste-derived organic soil amendments and beneficial impacts on soil bacterial processes

Applied Soil Ecology, Год журнала: 2025, Номер 206, С. 105870 - 105870

Опубликована: Янв. 22, 2025

Язык: Английский

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