Plant Communications, Год журнала: 2024, Номер 5(12), С. 101071 - 101071
Опубликована: Авг. 24, 2024
Язык: Английский
Nature Plants, Год журнала: 2025, Номер unknown
Опубликована: Янв. 16, 2025
Язык: Английский
Процитировано
2
Planta, Год журнала: 2024, Номер 259(5)
Опубликована: Апрель 9, 2024
Abstract Main conclusion In this review, we give an overview of plant sequencing efforts and how impacts functional genomics research. Plant genome sequence information greatly facilitates the studies biology, genomics, evolution genomes genes, domestication processes, phylogenetic relationships, among many others. More than two decades have boosted number available sequenced genomes. The first genome, Arabidopsis, was published in year 2000 currently, 4604 from 1482 species been published. Various large initiatives are running, which planning to produce tens thousands near future. on status use different research areas.
Язык: Английский
Процитировано
5
Journal of Systematics and Evolution, Год журнала: 2025, Номер unknown
Опубликована: Янв. 17, 2025
Abstract Plant miRNAs exhibit a dynamic and complex evolutionary landscape. Despite their rapid turnover, play crucial roles in regulating key biological processes, underscoring functional significance even when evolutionarily transient. This review explores the phenomenon of miRNA turnover through comprehensive survey conservation across land plants. We discuss how de novo overcome challenges such as transcriptional activation, structural requirements for biogenesis, integration into gene regulatory networks. Furthermore, we mechanisms origination, including inverted duplication target genes, derivation from retrotransposons or DNA transposons, spontaneous evolution, newly proposed mechanism template switching mutations. Duplication existing plays significant role family expansion, driving diversification strengthening While loss has been preliminarily explored, its remain insufficiently understood. To address this, three detailed steps to advance research loss. provides an integrated perspective on gain, plant miRNAs, offering insights significance.
Язык: Английский
Процитировано
0
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Март 4, 2025
Язык: Английский
Процитировано
0
Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)
Опубликована: Март 11, 2025
Abstract Key message Through meta-genetic analysis of Cucumis melo sweetness, we expand the description complex genetic architecture this trait. Integration extensive new results with published QTL data provides an outline towards construction a melon sweetness pan-QTLome. An ultimate objective in crop genetics is describing complete repertoire genes and alleles that shape phenotypic variation quantitative trait within species. Flesh primary determinant fruit quality consumer acceptance melons . diverse species that, among other traits, displays total soluble solids (TSS) content flesh, ranging from 2 0 Brix non-sweet to 18 sweet accessions. We present here TSS sugar melon, using six different populations measurements collected more than 30,000 open-field greenhouse-grown plants, integrated 15 sweetness-related loci (QTL) studies. Starting characterization composition across 180 accessions represent 3 subspecies 12 their cultivar-groups, mapped QTLs, confirmed sucrose accumulation key variable explaining variation. All modes-of-inheritance for were displayed by multi-season broad half-diallel population derived 20 founders, significant prevalence additive component. parallel mapping four advanced bi-parental populations, identified common as well unique QTLs chromosomal regions. demonstrate cumulative less-than-additive nature favorable potential QTL-stacking approach. Using our dataset, additionally able show weak correlations size ripening behavior, supporting effective breeding per se. Our analysis, combined additional layers data, broadens perspective on landscape proposes scheme future community-driven
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2496 - 2496
Опубликована: Март 11, 2025
Plukenetia volubilis L., a woody oilseed plant rich in α-linolenic acid, represents promising source of polyunsaturated fatty acids. However, the lack an efficient genetic transformation system has significantly hindered gene function research and molecular breeding P. volubilis. In this study, we developed highly Agrobacterium rhizogenes-mediated hairy root for via use gel combination with visually detectable RUBY reporter analysis roots. The results indicate that optimal method involves infecting seedlings containing acetosyringone inducing formation perlite. This approach resulted more than 18.97% producing positive roots overexpressing gene. Using system, successfully overexpressed antimicrobial peptide-encoding CEMA roots, which enhanced resistance to Fusarium oxysporum. Furthermore, by combining CRISPR-Cas9 tool, validated regulatory role PvoSHR development epidermal cells Unexpectedly, 123-bp DNA fragment from T-DNA region A. rhizogenes Ri plasmid was found be knocked genome, replacing 110-bp at induced double-strand breaks. Conclusively, provides powerful tool novel insights into editing systems other plants.
Язык: Английский
Процитировано
0
The Plant Journal, Год журнала: 2025, Номер 121(6)
Опубликована: Март 1, 2025
SUMMARY Bryophytes, which include mosses, liverworts, and hornworts, have evolved a highly successful strategy for thriving in terrestrial environments, allowing them to occupy nearly every land ecosystem. Their success is due unique combination of biochemical adaptations, diverse structural forms, specialized life cycle strategies. The key their evolutionary lies genomic diversity. To fully decode this diversity, the use advanced genome engineering techniques crucial. In review, we explore diversity bryophytes latest advancements studies engineering, ranging from precise gene editing whole‐genome synthesis. Notably, moss Physcomitrium patens stands out as only plant capable efficiently utilizing homologous recombination engineering. This capability has heralded new era synthetic genomics. By focusing on bryophytes, emphasize potential benefits unraveling genetic traits, could significant implications across various scientific fields, fundamental biology biotechnological applications.
Язык: Английский
Процитировано
0
BMC Plant Biology, Год журнала: 2025, Номер 25(1)
Опубликована: Март 19, 2025
Saline-alkali stress is one of the main abiotic stresses that constrains plant growth. Understanding response mechanism ornamental plants to saline-alkali great significance for improving landscape greening. Chrysanthemum a good with strong resistance stress, rich colors and easy management. Using TMT quantitative proteomics technology, leave root were either untreated or treated 200 mM NaCl 12 h, screened differentially expressed proteins. The results showed 66 452 differential proteins present in leaves roots after salt treatment, respectively. GO function mainly related carbohydrate energy metabolism, hormone response, antioxidant membrane protein activity. KEGG metabolic pathway concentrated glycine glutathione pathway, carbon fixation prokaryotes, 2-oxy-carboxylic acid metabolism. Combining transcripto-proteomics, analyses revealed significant enrichment starch anabolic catabolism, redox processes, ion homeostatic transport, phenylpropane biosynthesis. Under active pathways metabolism enable accumulate more substances improve capacity, which may play safeguarding role maintaining growth development mitigating reactive oxygen species damage under stress. purpose this study was screen key regulatory networks through proteomic assay, reveal molecular research not only provides resources salt-tolerant breeding but also offers theoretical support agricultural production ecological environmental protection.
Язык: Английский
Процитировано
0
aBIOTECH, Год журнала: 2025, Номер unknown
Опубликована: Март 28, 2025
Abstract Innovations in DNA sequencing technologies have greatly boosted population-level genomic studies plants, facilitating the identification of key genetic variations for investigating population diversity and accelerating molecular breeding crops. Conventional methods analysis typically rely on small variants, such as SNPs indels, use single linear reference genomes, which introduces biases reduces performance highly divergent regions. By integrating level sequences, pangenomes, particularly graph offer a promising solution to these challenges. To date, numerous algorithms been developed constructing pangenome graphs, aligning reads performing variant genotyping based graphs. As demonstrated various plant pangenomic studies, advancements allow detection previously hidden especially structural thereby enhancing applications mapping agronomically important genes. However, noteworthy challenges remain be overcome applying approaches plants. Addressing issues will require development more sophisticated tailored specifically Such improvements contribute scalability this approach, production super-pangenomes, hundreds or even thousands de novo–assembled genomes from one species genus can integrated. This, turn, promote broader pan-omic further advancing our understanding driving innovations crop breeding.
Язык: Английский
Процитировано
0
Journal of Sustainable Agriculture and Environment, Год журнала: 2025, Номер 4(2)
Опубликована: Март 31, 2025
ABSTRACT To fulfil food and nutritional demand for nine billion people by the mid‐21st century, global production must increase 60% regardless of challenges such as environmental pollution, water scarcity land degradation. Climate change exacerbates frequency intensity biotic abiotic stresses, which, in turn, severely compromise crop yields, jeopardize supply, deteriorate sustainable development goals achieving safety, limit climate‐smart production. Current consumption practices negatively influence environment, posing a major threat to ecosystem human health. Addressing these critical issues achieve agriculture necessitates designing future crops employing cutting‐edge breeding strategies enhanced productivity with minimal footprints. This endeavour requires comprehensive understanding plant stress adaptation, signalling pathways mitigation mechanisms. In this review, we first explain diverse impacts ongoing climate events on Subsequently, outline various tackle change, including agronomic practices, advanced technologies physiological molecular mechanisms tolerance. We also discuss engineering superior tolerance disease resistance nurturing healthy microbial partnerships between plants soil ensure nutrition security current populations amidst mounting challenges.
Язык: Английский
Процитировано
0