Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana
PLoS Genetics,
Год журнала:
2025,
Номер
21(1), С. e1011451 - e1011451
Опубликована: Янв. 13, 2025
Gamete
killers
are
genetic
loci
that
distort
segregation
in
the
progeny
of
hybrids
because
killer
allele
promotes
elimination
gametes
carry
sensitive
allele.
They
widely
distributed
eukaryotes
and
important
for
understanding
genome
evolution
speciation.
We
had
previously
identified
a
pollen
between
two
distant
natural
accessions
Arabidopsis
thaliana
.
This
involves
three
genetically
linked
genes,
we
reported
identification
gene
encoding
antidote
protects
grains
from
activity.
In
this
study,
other
genes
by
using
CRISPR-Cas9
induced
mutants.
These
necessary
activity
demonstrated
to
be
specific
pollen.
The
cellular
localization
encoded
proteins
suggests
mitochondria.
Sequence
analyses
reveal
predicted
domains
same
families
proteins.
addition,
C-terminal
half
one
is
identical
antidote,
amino
acid,
crucial
activity,
also
essential
function.
Investigating
more
than
700
worldwide
A
,
confirmed
locus
subject
structural
rearrangements
copy
number
variation.
By
exploiting
available
de
novo
genomic
sequences,
propose
scenario
emergence
Furthermore,
report
co-occurrence
behavior
genotypes
several
local
populations,
prerequisite
studying
gamete
wild.
highlights
potential
model
not
only
functional
studies
but
investigating
their
evolutionary
trajectories
at
complementary
geographical
scales.
Язык: Английский
Gene drive-based population suppression in the malaria vector Anopheles stephensi
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 24, 2025
Gene
drives
are
alleles
that
can
bias
the
inheritance
of
specific
traits
in
target
populations
for
purpose
modification
or
suppression.
Here,
we
construct
a
homing
suppression
drive
major
urban
malaria
vector
Anopheles
stephensi
targeting
female-specific
exon
doublesex,
incorporating
two
gRNAs
and
nanos-Cas9
to
reduce
functional
resistance
improve
female
heterozygote
fitness.
Our
results
show
was
recessive
sterile
both
females
males,
with
various
intersex
phenotypes
homozygotes.
Both
male
heterozygotes
only
moderate
conversion,
indicating
nanos
promoter
has
lower
activity
A.
than
gambiae.
By
amplicon
sequencing,
detect
very
low
level
allele
formation.
Combination
vasa-Cas9
line
boosts
conversion
rate
100%,
suggesting
use
similar
systems
population
continuous
release
strategy
SIT
fsRIDL
techniques.
This
study
contributes
valuable
insights
development
more
efficient
environmentally
friendly
pest
control
tools
aimed
at
disrupting
disease
transmission.
their
spread
through
populations.
constructed
efficiency
2-gRNA
mosquito
doublesex.
With
vasa-Cas9,
high
self-limiting
achieved.
Язык: Английский
Applications and status of gene drive in plants
PLoS Biology,
Год журнала:
2025,
Номер
23(4), С. e3003148 - e3003148
Опубликована: Апрель 15, 2025
Gene
drive
can
modify
or
suppress
plant
populations,
offering
solutions
to
challenges
associated
with
globalization
and
climate
change.
However,
common
features
of
biology
complicate
its
application.
Self-limiting
methods
provide
a
controlled,
reversible
path
forward.
Язык: Английский
Synthetic Gene Drives in Plants: Development Strategies, Potential Applications, and Ethical Considerations
Modern Agriculture,
Год журнала:
2024,
Номер
2(2)
Опубликована: Авг. 13, 2024
ABSTRACT
We
are
facing
significant
agricultural
and
environmental
challenges,
including
herbicide‐resistant
weeds
that
compromise
crop
yields
invasive
plant
species
disrupt
local
ecosystems.
Traditional
breeding
methods
increasingly
seen
as
ineffective
in
the
face
of
these
issues.
One
innovative
approach
involves
use
gene
drives,
a
technology
enables
targeted
genetic
modifications
to
transmit
at
super
Mendelian
rate
(>50%)
therefore,
rapidly
propagate
through
natural
populations.
In
this
perspective,
we
introduce
principles
underlying
our
development
synthetic
toxin‐antidote
drive,
CAIN
(CRISPR‐Assisted
Inheritance
utilising
NPG1
).
evaluate
potential
drives
alter
genetics
weed
populations
or
suppress
explore
various
factors
influence
effectiveness
emergence
suppressor
mutations
life‐history
traits
such
sexual
asexual
reproduction
patterns,
seed
pollen
dispersal
methods,
variability
generation
times.
Additionally,
discuss
biosafety
concerns
related
drive
experiments
field
releases,
strategies
counteract
unwanted
ethical
implications,
particularly
considering
risk
intentional
misuse
need
for
robust
regulatory
monitoring
frameworks.
Язык: Английский
Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 13, 2024
Population-scale
genome
modification
can
alter
the
composition
or
fate
of
wild
populations.
Synthetic
gene
drives
provide
one
set
tools,
but
their
use
is
complicated
by
scientific,
regulatory,
and
social
issues
associated
with
transgene
persistence
flow.
Here
we
propose
an
alternative
approach.
An
Allele
Sail
consists
a
editor
(the
Wind)
that
introduces
DNA
sequence
edits,
inherited
in
Mendelian
fashion.
Meanwhile,
edits
Sail)
experience
arithmetic,
Super-Mendelian
increase
frequency.
We
model
this
system
identify
contexts
which
single,
low
frequency
release
brings
to
very
high
also
conditions
manipulation
sex
determination
bring
about
population
suppression.
In
regulatory
frameworks
distinguish
between
transgenics
(GMO)
edited
non-transgenic
progeny
(non-GMO)
Sails
may
prove
useful
since
spread
GM
component
be
limited.
authors
introduce
as
method
for
spreading
genetic
changes
throughout
population.
Язык: Английский
Cleave and Rescue gamete killers create conditions for gene drive in plants
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 14, 2023
Abstract
Gene
drive
elements
promote
the
spread
of
linked
traits,
even
when
their
presence
confers
a
fitness
cost
to
carriers,
and
can
be
used
change
composition
or
fate
wild
populations.
Cleave
Rescue
(
ClvR
)
sit
at
fixed
chromosomal
position
include
DNA
sequence-modifying
enzyme
such
as
Cas9/gRNAs
(the
Cleaver/Toxin)
that
disrupts
endogenous
versions
an
essential
gene,
recoded
version
gene
resistant
cleavage
Rescue/Antidote).
spreads
by
creating
conditions
in
which
those
lacking
die
because
they
lack
functional
gene.
We
demonstrate
features
plant
Arabidopsis
thaliana
through
killing
gametes
fail
inherit
targets
YKT61,
whose
expression
is
required
male
female
for
survival.
Resistant
(uncleavable
but
functional)
alleles,
slow
prevent
drive,
were
not
observed.
Modeling
shows
s
are
likely
robust
certain
failure
modes
rapidly
population
modification
suppression.
Possible
applications
breeding,
weed
control,
conservation
discussed.
Язык: Английский
A horizon scan of biological conservation issues for 2025
Trends in Ecology & Evolution,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 10, 2024
Abstract
Gamete
killers
are
genetic
loci
that
distort
segregation
in
the
progeny
of
hybrids
because
killer
allele
promotes
elimination
gametes
carry
sensitive
allele.
They
widely
distributed
eukaryotes
and
important
for
understanding
genome
evolution
speciation.
We
had
previously
identified
a
pollen
between
two
distant
natural
accessions
Arabidopsis
thaliana
.
This
involves
three
genetically
linked
genes,
we
reported
identification
gene
encoding
antidote
protects
grains
from
activity.
In
this
study,
other
genes
by
using
CRISPR-Cas9
induced
mutants.
These
necessary
activity
demonstrated
to
be
specific
pollen.
The
cellular
localization
encoded
proteins
indicates
mitochondria.
Sequence
analyses
reveal
predicted
domains
same
families
proteins.
addition,
C-terminal
half
one
is
identical
antidote,
amino
acid,
crucial
activity,
also
essential
function.
Investigating
more
than
700
worldwide
A.
,
confirmed
locus
subject
structural
rearrangements
copy
number
variation.
By
exploiting
available
de
novo
genomic
sequences,
propose
scenario
emergence
Furthermore,
report
co-occurrence
behavior
genotypes
several
local
populations,
prerequisite
studying
gamete
wild.
highlights
potential
model
not
only
functional
studies
but
investigating
their
evolutionary
trajectories
at
complementary
geographical
scales.
Author
Summary
Certain
elements
qualified
as
selfish
they
favor
transmission
during
reproduction
detriment
do
them.
widespread
fungi
well
plants
or
animals,
made
up
even
components,
which
each
species.
Therefore,
must
studied
on
case-by-case
basis.
Moreover,
how
appear
propagate
population
remains
major
issue
biology.
Here
have
characterized,
plant
Arabidopsis,
involved
such
an
element,
called
killer.
targets
mitochondria
cause
death
it.
investigated
hundred
collected
worldwide,
giving
us
global
view
diversity
species
level.
found
some
French
populations
contain
both
plants,
constitutes
invaluable
resource
Язык: Английский
Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(21), С. 11792 - 11792
Опубликована: Ноя. 2, 2024
Forests
worldwide
are
under
increasing
pressure
from
climate
change
and
emerging
diseases,
threatening
their
vital
ecological
economic
roles.
Traditional
breeding
approaches,
while
valuable,
inherently
slow
limited
by
the
long
generation
times
existing
genetic
variation
of
trees.
CRISPR
technologies
offer
a
transformative
solution,
enabling
precise
efficient
genome
editing
to
accelerate
development
climate-resilient
productive
forests.
This
review
provides
comprehensive
overview
applications
in
forestry,
exploring
its
potential
for
enhancing
disease
resistance,
improving
abiotic
stress
tolerance,
modifying
wood
properties,
accelerating
growth.
We
discuss
mechanisms
various
systems,
including
base
editing,
prime
multiplexing
strategies.
Additionally,
we
highlight
recent
advances
overcoming
key
challenges
such
as
reagent
delivery
plant
regeneration,
which
crucial
successful
implementation
also
delve
into
ethical
considerations
using
gene
drive
population-level
alterations,
well
importance
containment
strategies
mitigating
risks.
emphasizes
need
continued
research,
technological
advancements,
extensive
long-term
field
trials,
public
engagement,
responsible
innovation
fully
harness
power
shaping
sustainable
future
Язык: Английский