Integrating Micro- and Nanostructured Platforms and Biological Drugs to Enhance Biomaterial-Based Bone Regeneration Strategies

Biomacromolecules, Год журнала: 2024, Номер 26(1), С. 140 - 162

Опубликована: Дек. 2, 2024

Bone defects resulting from congenital anomalies and trauma pose significant clinical challenges for orthopedics surgeries, where bone tissue engineering (BTE) aims to address these by repairing that fail heal spontaneously. Despite numerous advances, BTE still faces several challenges, i.e., difficulties in detecting tracking implanted cells, high costs, regulatory approval hurdles. Biomaterials promise revolutionize grafting procedures, heralding a new era of regenerative medicine advancing patient outcomes worldwide. Specifically, novel bioactive biomaterials have been developed promote cell adhesion, proliferation, differentiation osteoconductive osteoinductive characteristics, stimulating regeneration repair, particularly complex skeletal caused trauma, degeneration, neoplasia. A wide array biological therapeutics emerged, drawing the diverse spectrum gene therapy, immune interactions, RNA molecules. This review will provide insights into current state potential future strategies regeneration.

Язык: Английский

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The guanine nucleotide exchange factor DOCK5 negatively regulates osteoblast differentiation and BMP2-induced bone regeneration via the MKK3/6 and p38 signaling pathways

Experimental & Molecular Medicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Abstract DOCK5 (dedicator of cytokinesis 5), a guanine nucleotide exchange factor for Rac1, has been implicated in BMP2-mediated osteoblast differentiation, but its specific role osteogenesis and bone regeneration remained unclear. This study investigated the effect on using C21, chemical inhibitor, Dock5 -deficient mice. Osteoblast differentiation were analyzed marrow mesenchymal stem cells (BMSCs) various animal models. C21 significantly enhanced mineral deposition mouse MC3T3-E1 human BMSCs. knockout (KO) mice exhibited increased mass apposition rate, with their BMSCs showing differentiation. Calvarial defect ectopic formation models demonstrated significant induction KO compared to wild-type (WT) Moreover, inhibition by WT BMP2-induced subcutaneous formation. The mechanism responsible induced may involve suppression Rac1 under TAK1, accompanied activation MKK3/6 p38 BMP2. These findings strongly suggest that negatively regulates through signaling pathways involving MKK3/6, p38, providing new insights into potential therapeutic strategies regeneration.

Язык: Английский

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Structurally Defined Cartilaginous MEW-Assembloids for Critical-size Long Bone Healing

Biomaterials, Год журнала: 2025, Номер 319, С. 123202 - 123202

Опубликована: Фев. 19, 2025

Язык: Английский

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Modeling and fabrication of MEW-3D tubular scaffolds with tendon mechanical behavior for tenocyte differentiation

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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3D-printed polycaprolactone scaffolds functionalized with poly(lactic-co-glycolic) acid microparticles enhance bone regeneration through tunable drug release

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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A Wood‐Derived Periosteum for Spatiotemporal Drug Release: Boosting Bone Repair through Anisotropic Structure and Multiple Functions

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(19)

Опубликована: Апрель 2, 2024

Abstract Existing artificial periostea face many challenges, including difficult‐to‐replicate anisotropy in mechanics and structure, poor tissue adhesion, neglected synergistic angiogenesis osteogenesis. Here, inspired by natural wood (NW), a wood‐derived elastic periosteum is developed to mimic the structure functions of periosteum, which combines an (EW) skeleton, polydopamine (PDA) binder layer, layer‐by‐layer (LBL) biofunctional layers. Specifically, EW derived from NW utilized as anisotropic skeleton guide cell directional behaviors, moreover, it also shows similar modulus flexibility periosteum. To further enhance its osteogenesis, surface LBL layers are designed serve spatiotemporal release platforms achieve sequential long‐term pamidronate disodium (PDS) deferoxamine (DFO), pre‐encapsulated chitosan (CS) hyaluronic acid (HA) solutions, respectively. Furthermore, combined effect PDA coating enables tightly adhere damaged bone tissue. More importantly, this novel can boost formation vitro vivo. This study opens up new path for biomimetic design provides feasible clinical strategy repair.

Язык: Английский

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Exploring calcium-free alternatives in endochondral bone repair tested on In vivo trials - A review

Regenerative Therapy, Год журнала: 2024, Номер 26, С. 145 - 160

Опубликована: Июнь 1, 2024

Bone repair via endochondral ossification is a complex process for the critical size reparation of bone defects. Tissue engineering strategies are being developed as alternative treatments to autografts or allografts. Most approaches regeneration involve use calcium composites. However, exploring calcium-free alternatives in has emerged promising way contribute healing. By analyzing researches from last ten years, this review identifies potential benefits such compared traditional calcium-based approaches. Understanding impact on can have profound implications orthopedic and regenerative medicine. This evaluates efficacy through

Язык: Английский

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Biomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB for skull repair

Bone, Год журнала: 2024, Номер 190, С. 117315 - 117315

Опубликована: Ноя. 4, 2024

Tissue engineering employs the use of bioactive materials to facilitate filling and acceleration bone defect healing, thereby introducing novel concepts field in situ repair. Some studies have shown that periosteum plays an important role regeneration In this study, biomimetic periosteum-bone scaffolds were prepared by depositing poly-L-lactic acid (PLLA) electrospun fibers on surface gelatin/chitosan cryogel mimic structure, respectively. To improve bioactivity scaffold, morphogenetic protein-2 (BMP-2) was loaded into a loose porous mesh-like cryogel, while platelet-derived growth factor-BB (PDGF-BB) encapsulated core PLLA nanofibers with core-shell structure. Both these two factors released locally at site defect, where they exert synergistic effect osteogenesis, greatly accelerating healing. The vitro experiments demonstrated exhibited favourable biocompatibility osteogenesis ability. Furthermore, vivo indicated composite scaffold repaired rat skull defects more rapid effective manner. conclusion, codelivery BMP-2 PDGF-BB shows significant potential for regeneration.

Язык: Английский

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Periosteum Containing Implicit Stem Cells: A Progressive Source of Inspiration for Bone Tissue Regeneration

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2162 - 2162

Опубликована: Фев. 10, 2024

The periosteum is known as the thin connective tissue covering most bone surfaces. Its extrusive regeneration capacity was confirmed from very first century-old studies. Recently, pluripotent stem cells in with unique physiological properties were unveiled. Existing dynamic contexts and regulated by complex molecular networks, periosteal emerge having strong capabilities of proliferation multipotential differentiation. Through continuous exploration studies, we are now starting to acquire more insight into great potential formation repair situ or ectopically. It undeniable that developing further a promising strategy be harnessed regeneration. Here, summarized development structure periosteum, cell markers, biological features cells. Then, reviewed their pivotal role underlying regulation. understanding periosteum-related cellular content will help enhance future research efforts application transformation periosteum.

Язык: Английский

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Nature Knows Best: Extracellular Matrix‐Gelatin Hydrogel Masking Bioauthentic Bone Scaffold Locking Blood Clot to Induce M2 Macrophage‐Mediated Bone Regeneration via Activating an Endocytosis‐Macroautophagy‐Inflammation Axis

Small Structures, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 21, 2024

Individualized biological hydroxyapatite (BHA) scaffolds are essential for repairing critical craniomaxillofacial bone defects. Additive manufacturing relies on synthetic and biomimetic structures, making the resulting inferior to natural bone. A subtractive solution is proposed cut blocks then sinter them into BHA scaffolds. However, with fragile mechanical performance, fail maintain scaffold integrity, due microcracks generated by loss of organics from extracellular matrix (ECM). To preserve structure enhance properties, ECM‐gelatin hydrogel (ECM‐Gel) utilized as a micron‐level coating smooth surface like facial mask. ECM‐Gel shows potential in regulating blood clot fibrin network which may induce favorable macrophage‐mediated regeneration. Therefore, gelatin hydrogel‐masked (G‐BHA) system developed using crosslinker genipin (2 8 mM). formed “hybrid layer”, smoothing enhancing properties BHA; it activated platelets lock dense network, inducing M2 macrophage via endocytosis‐macroautophagy‐inflammation axis In vivo experiments confirmed G‐BHA promoted ectopic situ ECM‐gel‐masking demonstrates great developing individualized bioauthentic

Язык: Английский

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