Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(44)
Опубликована: Окт. 23, 2024
Exposure to brighter nights and darker days causes circadian disruption, which accompanies poor health outcomes that increase mortality risk. Whether personal day night light exposure predicts risk is not known. This study ...Light enhances or disrupts rhythms, depending on the timing of exposure. Circadian disruption contributes has been established. We ...
Язык: Английский
Journal of the American Chemical Society, Год журнала: 2025, Номер unknown
Опубликована: Фев. 2, 2025
Proteins are subject to aging in the form of spontaneous, nonenzymatic post-translational modifications (PTMs). One such PTM is formation β-linked isomer l-isoaspartic acid (isoAsp) from aspartic (Asp) or asparagine residues, which tends occur long-lived proteins. Histones can exhibit half-lives on order 100 days, and unsurprisingly, isoAsp has been observed nearly every histone family. Delineating molecular consequences histones challenging due multitude processes that time scales. To isolate effects a specific modification thus necessitates precise vitro characterization with well-defined substrates. Here, we adapt protein semisynthesis approach generate full-length variants H4 canonical Asp at position 24 replaced by its (H4isoD24). This variant was incorporated into chromatin templates, resulting constructs were used interrogate key parameters integrity maintenance vitro: compaction, nucleosome remodeling, methylation lysine 20 (H4K20). Remarkably, despite disruptive changes backbone's spacing direction, isoD24 did not dramatically disrupt Mg2+-mediated self-association repositioning remodeler Chd1. In contrast, H4isoD24 significantly inhibited both Set8- Suv4-20h1-catalyzed H4K20. These results suggest gives rise complex reorganization functional landscape, macroscopic show robustness local mechanisms vulnerability presence this mark.
Язык: Английский
Процитировано
0
Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Human Reproduction Update, Год журнала: 2025, Номер unknown
Опубликована: Фев. 20, 2025
Ovarian aging occurs earlier than the of many other organs and has a lasting impact on women's overall health well-being. However, effective interventions to slow ovarian remain limited, primarily due an incomplete understanding underlying molecular mechanisms drug targets. Recent advances in omics data resources, combined with innovative computational tools, are offering deeper insight into complexities aging, paving way for new opportunities discovery development. This review aims synthesize expanding multi-omics data, spanning genome, transcriptome, proteome, metabolome, microbiome, related from both tissue-level single-cell perspectives. We will specially explore how analysis these emerging datasets can be leveraged identify novel targets guide therapeutic strategies slowing reversing aging. conducted comprehensive literature search PubMed database using range relevant keywords: age at natural menopause, premature insufficiency (POI), diminished reserve (DOR), genomics, transcriptomics, epigenomics, DNA methylation, RNA modification, histone proteomics, metabolomics, lipidomics, single-cell, genome-wide association studies (GWAS), whole-exome sequencing, phenome-wide (PheWAS), Mendelian randomization (MR), epigenetic target, machine learning, artificial intelligence (AI), deep multi-omics. The was restricted English-language articles published up September 2024. Multi-omics have uncovered key driving including damage repair deficiencies, inflammatory immune responses, mitochondrial dysfunction, cell death. By integrating researchers critical regulatory factors across various biological levels, leading potential Notable examples include genetic such as BRCA2 TERT, like Tet FTO, metabolic sirtuins CD38+, protein BIN2 PDGF-BB, transcription FOXP1. advent cutting-edge technologies, especially technologies spatial provided valuable insights guiding treatment decisions become powerful tool aimed mitigating or As technology advances, integration AI models holds more accurately predict candidate convergence offers promising avenues personalized medicine precision therapies, tailored Not applicable.
Язык: Английский
Процитировано
0
Trends in Cell Biology, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
While mitochondrial dysfunction is one of the canonical hallmarks aging, it remains only vaguely defined. Its core feature embraces defects in energy-producing molecular machinery, respiratory complexes (MRCs). The causes and consequences these hold research attention. In this review, we assess lifecycle complexes, from biogenesis to degradation, look closely at mechanisms that could underpin their aged cells. We discuss how processes be altered by aging expand on fate MRCs age-associated pathologies. Given complexity behind MRC maintenance functionality, several traits contribute phenomenon known as dysfunction. New advances will help us better understand machinery age-related diseases.
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Процитировано
0
Current Opinion in Genetics & Development, Год журнала: 2025, Номер 92, С. 102347 - 102347
Опубликована: Апрель 14, 2025
Язык: Английский
Процитировано
0
Genes to Cells, Год журнала: 2024, Номер 29(10), С. 808 - 819
Опубликована: Июль 23, 2024
Abstract Mammalian oocytes undergo a long‐term meiotic arrest that can last for almost the entire reproductive lifespan. This occurs after DNA replication and is prolonged with age, which poses challenge to in maintaining replication‐dependent chromosomal proteins required completion of meiosis. In this study, we show histones are reduced age mouse oocytes. Both types histone H3 variants, H3.1/H3.2 replication‐independent H3.3, decrease age. Aging‐associated reduction associated transcriptomic features caused by genetic depletion H3.3. Neither nor H3.3 accelerates aging‐associated increase premature chromosome separation causes segregation errors. We suggest linked several abnormalities but does not significantly contribute errors during lifespan mice.
Язык: Английский
Процитировано
2
Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
1
Nature Aging, Год журнала: 2024, Номер 4(12), С. 1711 - 1730
Опубликована: Дек. 13, 2024
Язык: Английский
Процитировано
1
Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(8), С. 596 - 596
Опубликована: Июль 5, 2024
Язык: Английский
Процитировано
0