Protein degradation shapes developmental tempo in mouse and human neural progenitors

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

ABSTRACT The speed of embryonic development varies considerably between mammalian species, yet the underlying molecular mechanisms remain poorly understood. To investigate basis for species-specific developmental tempo, we performed a comprehensive comparative analysis protein dynamics in mouse and human neural progenitors (NPs). Through combination targeted labelling, quantitative mass spectrometry, depletion with self-labeling tags, demonstrate that degradation is key driver tempo differences NPs. We observe systematic 1.5-fold increase half-lives NPs compared to mouse, independent cellular compartment or function. This difference persists post-mitotic neurons, indicating active as primary mechanism. Proteasomal activity also ∼1.5-fold higher NPs, consistent upregulation proteasome-associated proteins. Importantly, increasing rate proteolytic transcriptional repressor accelerates expression its target gene. Despite rates, synthesis rates are similar resulting content Our findings highlight central role controlling provide insight into evolutionary changes timing across species.

Язык: Английский

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The idiosyncrasies of oocytes

Trends in Cell Biology, Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

Язык: Английский

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Protein research may hint at how human eggs survive for decades

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(44)

Опубликована: Окт. 23, 2024

Exposure to brighter nights and darker days causes circadian disruption, which accompanies poor health outcomes that increase mortality risk. Whether personal day night light exposure predicts risk is not known. This study ...Light enhances or disrupts rhythms, depending on the timing of exposure. Circadian disruption contributes has been established. We ...

Язык: Английский

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