Normalized and Directional Interplay Scoring for the Interrogation of Proteoform Data DOI
Karl F Poncha, Alyssa T. Paparella, Nicolas L. Young

и другие.

Journal of Proteome Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Histone proteoforms, often presenting multiple co-occurring post-translational modifications (PTMs), are central to chromatin regulation and gene expression. A proteoform is a specific form of protein that includes variations arising from genetic changes, alternative RNA splicing, proteolytic processing, PTMs. Genome-indexed histone proteoforms define the code, influencing cellular phenotype by dictating DNA interacting partners. Understanding dynamics essential for elucidating chromatin-based regulatory mechanisms. Advances in middle-down top-down proteomics enable accurate identification quantitation thousands single run. However, resulting data complexity presents significant challenges analysis visualization. Here, we introduce two new computational methods analyze PTMs demonstrate their use mouse organs during aging. The score term "normalized interplay" addresses limitations original crosstalk "interplay" providing more complete measure PTM crosstalk. second score, ΔI or "directional an asymmetric quantifying magnitude directionality between Applying our two-stage scoring approach CrosstalkDB reveals H3

Язык: Английский

Traversing the drug discovery landscape using native mass spectrometry DOI Creative Commons
Hannah M. Britt, Carol V. Robinson

Current Opinion in Structural Biology, Год журнала: 2025, Номер 91, С. 102993 - 102993

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Normalized and Directional Interplay Scoring for the Interrogation of Proteoform Data DOI
Karl F Poncha, Alyssa T. Paparella, Nicolas L. Young

и другие.

Journal of Proteome Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Histone proteoforms, often presenting multiple co-occurring post-translational modifications (PTMs), are central to chromatin regulation and gene expression. A proteoform is a specific form of protein that includes variations arising from genetic changes, alternative RNA splicing, proteolytic processing, PTMs. Genome-indexed histone proteoforms define the code, influencing cellular phenotype by dictating DNA interacting partners. Understanding dynamics essential for elucidating chromatin-based regulatory mechanisms. Advances in middle-down top-down proteomics enable accurate identification quantitation thousands single run. However, resulting data complexity presents significant challenges analysis visualization. Here, we introduce two new computational methods analyze PTMs demonstrate their use mouse organs during aging. The score term "normalized interplay" addresses limitations original crosstalk "interplay" providing more complete measure PTM crosstalk. second score, ΔI or "directional an asymmetric quantifying magnitude directionality between Applying our two-stage scoring approach CrosstalkDB reveals H3

Язык: Английский

Процитировано

0