Successional dynamics and alternative stable states in a saline activated sludge microbial community over 9 years

Microbiome, Год журнала: 2021, Номер 9(1)

Опубликована: Окт. 6, 2021

Abstract Background Microbial communities in both natural and applied settings reliably carry out myriads of functions, yet how stable these taxonomically diverse assemblages can be what causes them to transition between states remains poorly understood. We studied monthly activated sludge (AS) samples collected over 9 years from a full-scale wastewater treatment plant answer complex AS evolve the long term community functions change when there is disturbance operational parameters. Results Here, we show that microbial system fluctuated around average for 3 but was then abruptly pushed into an alternative state by simple transient (bleaching). While taxonomic composition rapidly turned new following disturbance, metabolic profile performance remained remarkably stable. A total 920 metagenome-assembled genomes (MAGs), representing approximately 70% ecosystem, were recovered 97 metagenomes. Comparative genomic analysis revealed increased ability aggregate cohorts MAGs with correlated dynamics are dominant after bleaching event. Fine-scale also dominated during different periods showed successional on seasonal longer time scales due temperature fluctuation gradual changes mean residence reactor, respectively. Conclusions Our work highlights assume under highly similar environmental conditions specific threshold may lead rapid shift composition.

Язык: Английский

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Genomic adaptations to an endolithic lifestyle in the coral-associated alga Ostreobium

Current Biology, Год журнала: 2021, Номер 31(7), С. 1393 - 1402.e5

Опубликована: Фев. 6, 2021

Язык: Английский

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Ecology of Endozoicomonadaceae in three coral genera across the Pacific Ocean

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июнь 1, 2023

Abstract Health and resilience of the coral holobiont depend on diverse bacterial communities often dominated by key marine symbionts Endozoicomonadaceae family. The factors controlling their distribution functional diversity remain, however, poorly known. Here, we study ecology at an ocean basin-scale sampling specimens from three genera ( Pocillopora , Porites Millepora ) 99 reefs 32 islands across Pacific Ocean. analysis 2447 metabarcoding 270 metagenomic samples reveals that each genus harbored a distinct new species . These are composed nine lineages have biogeographic patterns. most common one, found in appears to be globally distributed symbiont with metabolic capabilities, including synthesis amino acids vitamins not produced host. other structured partly host genetic lineage mainly geographic location is more rarely associated Our results show different exhibit strategies host- associations defined bacteria level.

Язык: Английский

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Diversity of the Pacific Ocean coral reef microbiome

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июнь 1, 2023

Coral reefs are among the most diverse ecosystems on Earth. They support high biodiversity of multicellular organisms that strongly rely associated microorganisms for health and nutrition. However, extent coral reef microbiome diversity its distribution at oceanic basin-scale remains to be explored. Here, we systematically sampled 3 morphotypes, 2 fish species, planktonic communities in 99 from 32 islands across Pacific Ocean, assess composition biogeography. We show a very large richness compared other environments, which extrapolated all fishes corals Pacific, approximates current estimated total prokaryotic entire Microbial vary within animal biomes (coral, fish, plankton), geographically. For corals, cross-ocean patterns different those known organisms. Within each morphotype, community is always determined by geographic distance first, both island ocean scale, then environment. Our unprecedented sampling effort microbiomes, as part Tara expedition, provides new insight into global microbial diversity, factors driving their distribution, biocomplexity ecosystems.

Язык: Английский

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Multi-environment ecogenomics analysis of the cosmopolitan phylum Gemmatimonadota

Microbiology Spectrum, Год журнала: 2023, Номер 11(5)

Опубликована: Сен. 21, 2023

Gemmatimonadota is a diverse bacterial phylum commonly found in environments such as soils, rhizospheres, fresh waters, and sediments. So far, the contains just six cultured species (five of them sequenced), which limits our understanding their diversity metabolism. Therefore, we analyzed over 400 metagenome-assembled genomes (MAGs) 5 culture-derived representing from various aquatic environments, hydrothermal vents, sediments, host-associated (with marine sponges coral) species. The principal coordinate analysis based on presence/absence genes phylogenomic documented that were most distant freshwater wastewater A smaller genome size coding sequences (CDS) number reduction observed MAGs, pointing to an oligotrophic environmental adaptation. Several metabolic pathways are restricted specific environments. For example, for anoxygenic phototrophy only freshwater, wastewater, soda lake sediment genomes. There several sediments containing type IC/ID ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Various harbored II RuBisCO, whereas RuBisCO-like protein was soil, host-associated, does not contain nitrogen fixation genes; however, nosZ gene, involved N2O, present missing water Gemmatimonadota. presented data suggest evolved organotrophic relying aerobic respiration then remodeled its inventory when adapting particular IMPORTANCE rarely studied consisting handful Recent culture-independent studies these organisms distributed many including marine, fresh, waste waters. However, due lack species, information about potential role scarce. collected different habitats performed systematic genomic characteristics potential. Our results show how have adapted

Язык: Английский

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