Beyond resistance: antifungal heteroresistance and antifungal tolerance in fungal pathogens

Current Opinion in Microbiology, Год журнала: 2024, Номер 78, С. 102439 - 102439

Опубликована: Фев. 23, 2024

Язык: Английский

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Mechanisms underlying the interactions and adaptability of nitrogen removal microorganisms in freshwater sediments

Advanced Biotechnology, Год журнала: 2024, Номер 2(3)

Опубликована: Июнь 17, 2024

Microorganisms in eutrophic water play a vital role nitrogen (N) removal, which contributes significantly to the nutrient cycling and sustainability of ecosystems. However, mechanisms underlying interactions adaptation strategies N removal microorganisms ecosystems remain unclear. We thus analyzed field sediments collected from freshwater ecosystem, enriched microorganisms, examined their function adaptability through amplicon, metagenome metatranscriptome sequencing. found that activities could be affected potential competition inhibition among microbial metabolic pathways. High-diversity communities generally increased abundance expression functional genes. Further enrichment experiments showed led development simplified but more stable communities, characterized by similar evolutionary patterns tighter interactions, adaptability. Notably, sustained provision NH4+ NO2- during potentially strengthen interconnections denitrification, anaerobic ammonium oxidation (anammox) dissimilatory nitrate reduction (DNRA) processes. Moreover, identification shared traits anammox DNRA implies important cooperative associations microorganisms. Our findings highlight affect adaptive key taxa involved removal.

Язык: Английский

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Metabolic mutations reduce antibiotic susceptibility of E. coli by pathway-specific bottlenecks

Molecular Systems Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Язык: Английский

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Strategy to improve anaerobic fermentation performance of lactate-rich wastewater by combining biochar augmentation and acetate supplementation

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 159782 - 159782

Опубликована: Янв. 1, 2025

Язык: Английский

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Root exudates and microbial metabolites: signals and nutrients in plant-microbe interactions

Science China Life Sciences, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Язык: Английский

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Advances in linking single-cell bacterial stress response to population-level survival

Current Opinion in Biotechnology, Год журнала: 2023, Номер 79, С. 102885 - 102885

Опубликована: Янв. 13, 2023

Язык: Английский

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Identification of robust and generalizable biomarkers for microbiome-based stratification in lifestyle interventions

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Авг. 8, 2023

Abstract Background A growing body of evidence suggests that the gut microbiota is strongly linked to general human health. Microbiome-directed interventions, such as diet and exercise, are acknowledged a viable achievable strategy for preventing disorders improving However, due significant inter-individual diversity between subjects, lifestyle recommendations expected have distinct highly variable impacts microbiome structure. Results Here, through large-scale meta-analysis including 1448 shotgun metagenomics samples obtained longitudinally from 396 individuals during studies, we revealed Bacteroides stercoris , Prevotella copri vulgatus biomarkers microbiota’s resistance structural changes, aromatic non-aromatic amino acid biosynthesis important regulator dynamics. We established criteria distinguishing compositional changes normal fluctuation classified based on their level response. further developed machine learning model predicting “responders” “non-responders” independently type intervention with an area under curve up 0.86 in external validation cohorts different ethnicities. Conclusions propose here microbiome-based stratification possible identifying plastic or resistant microbial structures. Identifying subjects will not respond generalized therapeutic interventions targeting restructuring ensure primary end-points clinical studies reached.

Язык: Английский

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Interspecies interactions determine growth dynamics of biopolymer-degrading populations in microbial communities

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(44)

Опубликована: Окт. 25, 2023

Microbial communities perform essential ecosystem functions such as the remineralization of organic carbon that exists biopolymers. The first step in mineralization is performed by biopolymer degraders, which harbor enzymes can break down polymers into constituent oligo- or monomeric forms. released nutrients not only allow degraders to grow, but also promote growth cells either consume degradation products, i.e., exploiters, metabolites scavengers. It currently clear how remineralizing assemble at microscale—how interactions between different guilds influence their and spatial distribution, hence development dynamics community. Here, we address this knowledge gap studying marine microbial grow on abundant alginate. We used batch assays microfluidics coupled time-lapse microscopy quantitatively investigate distribution single cells. found presence exploiters scavengers alters degrader In general, scavengers—which collectively refer cross-feeder cells—slowed addition, coexistence with cross-feeders altered production extracellular Our findings reveal ecological nondegrading community members have a profound impact remineralize biopolymers nature.

Язык: Английский

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Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC

Nature Microbiology, Год журнала: 2023, Номер 8(3), С. 441 - 454

Опубликована: Фев. 16, 2023

Abstract Genetically identical cells are known to differ in many physiological parameters such as growth rate and drug tolerance. Metabolic specialization is believed be a cause of phenotypic heterogeneity, but detection metabolically divergent subpopulations remains technically challenging. We developed proteomics-based technology, termed differential isotope labelling by amino acids (DILAC), that can detect producer consumer particular acid within an isogenic cell population monitoring peptides with multiple occurrences the acid. reveal young, morphologically undifferentiated yeast colonies contain lysine producers consumers emerge due nutrient gradients. Deconvoluting their proteomes using DILAC, we find evidence for situ cross-feeding where rapidly growing ferment provide more slowly growing, respiring ethanol. Finally, combining DILAC fluorescence-activated sorting, show metabolic diverge phenotypically, exemplified different tolerance antifungal amphotericin B. Overall, captures previously unnoticed heterogeneity provides experimental role interactions source populations.

Язык: Английский

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Citric Acid Confers Broad Antibiotic Tolerance through Alteration of Bacterial Metabolism and Oxidative Stress

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 9089 - 9089

Опубликована: Май 22, 2023

Antibiotic tolerance has become an increasingly serious crisis that seriously threatened global public health. However, little is known about the exogenous factors can trigger development of antibiotic tolerance, both in vivo and vitro. Herein, we found addition citric acid, which used many fields, obviously weakened bactericidal activity antibiotics against various bacterial pathogens. This mechanistic study shows acid activated glyoxylate cycle by inhibiting ATP production bacteria, reduced cell respiration levels, inhibited tricarboxylic (TCA cycle). In addition, oxidative stress ability led to imbalance oxidation–antioxidant system. These effects together induced bacteria produce tolerance. Surprisingly, succinic xanthine could reverse vitro animal infection models. conclusion, these findings provide new insights into potential risks usage relationship between metabolism.

Язык: Английский

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