Naked eye biosensors for pathogen monitoring

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 171, С. 117499 - 117499

Опубликована: Дек. 21, 2023

Язык: Английский

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Carl Woese: Still ahead of our time

mLife, Год журнала: 2022, Номер 1(4), С. 359 - 367

Опубликована: Дек. 1, 2022

The work of Carl Woese and his colleagues the Urbana University has been one most important breakthroughs in biology last century (for historical sketches, see Refs.1, 2). pioneered use macromolecule sequences to decipher relationships between all organisms, fulfilling Darwin's dream get a "fairly true genealogical trees each great kingdom nature"3. He went even beyond this objective since he was finally able identify connect nature (which called domains) into single tree. apex discovery 1977 an entire new domain life, hidden before our eyes, Archaea4. 16S ribosomal RNA (rRNA) as molecular chronometer later probe for PCR not only revealed third life but also paved way detection identification microorganisms that were yet amenable cultivation5. I deeply affected when passed away 10 years ago because my three scientific heroes: together with James Watson, DNA double-helix, Wang, topoisomerases (the Ws). surprised still shocked despite monumental achievements, death completely ignored French media, suspect it same countries worldwide. Notably, died day famous horse, Ourasi, had won several competitions such "le grand prix de l'arc Triumphe." horse made headlines media day, including prime-time TV news. could resist sending short piece entitled "the scientist horse" Journal Liberation which emphasize difference treatment (and like horses) greatest scientists century. It published online version journal link now removed, you can read at end fourth chapter book, Microbes from Hell1. ardent activist promoting vision living world by discoveries. criticized misleading prokaryotic/eukaryotic paradigm, classification organisms is based on phenotypic features, sequel anthropic lower higher ones, humans top classifications6. paradigm establish 15 Archaea7. Therefore, George Fox their seminar paper Proc Natl Acad Sci USA 1977, already firmly established. prokaryote/eukaryote easy explain (with or without nucleus) quickly became pillar cellular organisms8. dichotomy welcomed cytologists biologists alike modern classification, be opposed five-kingdom favored zoologists botanists9. This probably explains why so many biologists, strongly time Woese's proposal though data2, 10, 11 (see Ref.12 answer Ernst Mayer). Why should we change winning team? Here, will briefly describe various encounters Woese, focusing agreements disagreements concerning universal tree Last Universal Common Ancestor (LUCA), which, according definitely less recognized major question today"13. discuss how legacy challenged occasions reactions. Unfortunately, no longer among us defend legacy. All aware existence Archaea recognize merit revealing deep divide "prokaryotic world," too (in opinion) believe view fundamentally wrong favor pre-Woesian evolutionary model. dominant one, paralleled virus/phage virosphere, layman politicians journalists ignore (still confused Bacteria). More troublesome fact (except those working Archaea) are unaware vastly underestimate importance. seems remind generations importance history science fight ideas think they forefront science. discovered reading 1981 review Scientific American described "Archaebacteria" possibility studying "primary kingdom" determine common ancestor domains, progenote14. turning point career immediately stop replication Bacteria (Escherichia coli) started Archaea, polymerases. Thanks inspiration help few pioneers field, especially Wolfram Zillig15, rapidly accumulate significant results study Halobacterium halobium Sulfolobus acidocaldarius16, 17. first publications topic, invited Zillig participate second meeting organized Martinsried Institute, near Munich 1985. gave me opportunity time. vividly remember memorable sparring Lake took place meeting. Based electron microscopic pictures ribosomes, just two papers Science18, 19 proposed replacing "urkingdoms" (Figure 1A) four 1B). In unrooted tree, ribosome structure, Archaebacteria split "Archaebacteria proper," limited halophiles methanogens, urkingdom, Eocytes, grouping other thermophilic archaea, Thermococcus Thermoplasma18. Lake, who spoke first, violently attacked explained him different values versus genotypic data. came back shivering much argument opinion people present explanation. structural signatures Jim used define Eocyte indeed observed mesophilic methanogen, Methanococcus vanielli29. paper, although Science, cited today, whereas Lake's 1984 proponents two-domains (2D) hypothesis below) supporting favorite hypothesis30, 31. discouraged failure held Victoria Canadian east coast fresh arguments. meantime, learned phylogenetic techniques designed method own, parsimony32, produced rRNA Thermoplasma removed Eocytes33. If root localized bacteria obtains 2D trees, 1988 (Ref.33) clearly (instead 1984) description 1D). After talk meeting, stage conclude speaker brought arguments theory. striking contrast mute Canada, leaving coworker Gary Olsen against Lake34. seemed bit ongoing debate. How realize scenario good one? wondering mathematician here settle dispute. realized myself problem primarily mathematical (today, speaking about choose best model) more data sets22. fed up meetings eager person opponents. Besides conflict rejection theory evolutionists, Mayer Lynn Margulis, continue support paradigm2. My encounter thus delayed until 1994, very beginning genomic era. Despite reluctance attend meetings, joined friendship Zillig, influential bringing gospel Europe15. We waiting genome sequenced (most likely bacterial one), Roger Garret Aarhus Denmark35 small roundtable five colleagues, one) archaeal selected mammoth task. Each participant name archaeon floor expected (hoping would one). "We sequence least five," declared stupefaction incredulity. fact, right genomes available century, Craig Venter's team done collaboration 1996 (Methanocaldococcus vannielii)36. example lifetime. being heroes, did hesitate disagree him. strong proponent rooting groups 1980s37, 38. obtained combining analysis paralogous proteins originated gene duplication LUCA. For time, Hervé Philippe, these reliable attraction long branch separated corresponding respective outgroup39, 40. suspected preferred Bacteria. Indeed, rooted justify changing Archaea20 hoped topology simply extreme environments 1C). while, disagreed Eukaryotes domains. liked idea some eukaryotic spliceosome, possibly Of course, rejected have old division. When Philippe defended reporting presence bacterial-like genes than eukaryotic-like genomes39, ridiculed quantitative qualitative, quality hallmark translation apparatus studies41. soon right, allowing parsimonious distribution domains life27 1E). However, cannot solved analyses reasonable guess comparative (Box 1). LUCA smaller 33–34 ribosomes contain around 60–80 proteins27. mechanism biosynthesis simpler, protein involved process42. initiation transcription specific, factor set elongation Finn Werner)43. RNA, explaining replication, replicase, primase, helicase, homologous Arkarya Bacteria44 (Arkarya clade Eukarya)27. (processivity factor) might introduced independently lineages viruses. ATPase synthase ATP fermentation subunit essential synthesis Arkarya45. These reasoning sophistication fundamental mechanisms takes lineages. Alternative hypotheses (complex LUCA) involve replacement Arkaryal features ones vice versa ones. There obvious selection pressure suggested replacement, except simplification branch40. Although simpler progenote, sensu Fox21, 46, sophisticated membrane pumps capable faithful synthesis27. seminal "The concept evolution"46, unfaithful apparatus. Again, gap exists hand Eukarya evolution always parsimonious, remain open possible alternatives47. partly agree LUCA, do stricto sensu, translate mRNA message rather faithfully. conclusion contained transfer (tRNA) modifications crucial translation. A provided Kae1 Sua5 t6A biosynthesis, tRNA modification ANN codons. need additional factors perform task sufficient synthesize mitochondria, suggesting mitochondria recapitulates LUCA27. adopted promoted so-called "bacterial branch," never considered finding Eukarya. departure cladistic rules due fear weakening three-domain promoted. if follows rules, usually assumed, two-domain Bacteria, naming Arkarya27. Probably bypassing case 2002, Darwinian threshold transition period ancestors Archaeal Ancestor, LACA; Bacterial LBCA; Eukaryotic LECA), hereafter referred ancestors21. moment transmission genetic information moves predominantly horizontal mode lateral (LGT) vertical mode. assumed LGTs allow organisms. Accordingly, argue applicable threshold, separation leading Killing birds once, tempo dramatically decreased emergence ancestors. dramatic reduction observations early looking shape tree13. rate within extrapolated ancestors, born formation Earth! LGT prevalence correct rarely LGT48, 49. 2006, replicated repaired faithfully RNA50. viruses triggers independent RNA-to-DNA transitions, domain. To delight, reject agreed refer USA. respond critics challenge life. prevalent once thought, sometimes far apart led buzz forgetting trumpeting "Darwin wrong" discussion Ref.51). profoundly views, authors jokingly percent"52 networks53. save upper part (those context. chance meet another 2003 personal discussions evolution. little afraid knowledge previous disagreement negative impact encounter. case; friendly, real lover something give happy figure 1982, someone remembered review14. Lund Sweden honor awarded Crafoord prize Royal Swedish Academy Sciences. received King Sweden, Gustav XVI, descendant Marshal Napoleon Bernadotte, unexpected turn history. occasion humor mimicked official dinner ritual master ceremony Chap. 4 Ref.1). prize, 3 Nobel (both terms money ceremony!), exception complete absence press coverage. pity Prize. speak archaea. deserved medicine, considering markers microorganism medicine. recent Svante Pääbo sequencing Neanderthal confirms advances fields prize. wonder opposition adversaries evolutionists played role final distinction. met 2007 Illinois Urbana-Champaign celebrate 30th anniversary Archaea. "hidden your eyes" resembling visible, decades cousins under microscope. visit laboratory opening box containing film two-dimensional gel chromatography collection oligonucleotides after digestion Methanobacterium thermoautotrophicum RNase T1 1 Ref.15). honored own university surrounded friends introduce countries, Karl Stetter Germany, Yoshizumi Ishino Japan, David Prangishvili former USSR. 2012, reawakening Asgard archaea24, 25. am really reacted twist biology. imagine disturbed, view. decade, paraphyletic, considers belong 1F). make things worse, often depicted fusions (one mitochondria) archaeon, Margulis highly despised (figure Ref14). Archaebacteria, hence illustrated contrasting eukaryotes emerged fusion prokaryotes (only time)14. much. going past becoming one. classical during decade fast-evolving species sets, combined difficulty recovering testifying monophyly reviews results, Ref.54). does mean archaea breakthrough provide exciting understand Phylogenetic odd Signature Proteins (ESPs) extensive proto-eukaryotes54. Studying reconstruct proto-eukaryotic lineage. proto-eukaryotes suggests thriving LECA environments, indicating shed light type environment inhabited cultivated Candidatus Prometheoarchaem synthrophicum, turned out dependent growth its physical association methanogenic archaeon55. tour force concluded publication genomics "most them, all, symbiotic interactions." tempting suggest facilitated interactions Asgard54. One live ectosymbionts today. search associations biotopes adventure young (or young) microbial ecologists54. assault three-domains concept, deny prokaryotic At 2020, heralded putting nail coffin. From 10,575 genomes, 381 markers, "1.16 trillions non amino-acids," separates shorter previously thought56. branches separating altogether. correct, there reason separate reunified domain, pre-Woese area. Looking cursorily claim, flaws conclusion. included 6 34 shared proteins. They is, instance, gyrase, recruited (Ref. 50 references therein). subsequently refuted Moody identified both paralogy Zhu colleagues49. noticed 14 include priori unbelievable aim measure distance 68 others 25% sampled Strikingly, (a prerequisite kind analysis) recovered 22 trees49. indicated that, transferred other. review, Refs.23, 57) confirmed recently using data, non-ribosomal proteins22, 48, Long lengths criterion determining besides widespread domains48, 58. Failure consider Weiss colleagues59. reported 355 supposed starting "6.1 million protein-coding genes" "286,514 clusters." showed metabolism similar Clostridia genome, "prokaryote" progenote. thrived high temperatures reverse enzymes found mesophiles hyperthermophiles, gyrases formed monophyletic analysis, them short, gyrase intermixed, domains58. reanalysis colleagues' Berkemer McGlynn 82% all), LUCA48. again underrepresented low diversity. looked arrived Using criteria represented distantly related phylums, 15–20 "LUCA" (unpublished observations). proteome table. scenarios origin

Язык: Английский

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The expanding Asgard archaea invoke novel insights into Tree of Life and eukaryogenesis

mLife, Год журнала: 2022, Номер 1(4), С. 374 - 381

Опубликована: Дек. 1, 2022

Abstract The division of organisms on the Tree Life into either a three‐domain (3D) tree or two‐domain (2D) has been disputed for long time. Ever since discovery Archaea by Carl Woese in 1977 using 16S ribosomal RNA sequence as evolutionary marker, there great advance our knowledge not only growing diversity but also relationships between different lineages living organisms. Here, we present this perspective to summarize progress archaeal and changing notion Life. Meanwhile, provide latest genomics/physiology‐based Asgard closest relative Eukaryotes. Furthermore, propose three major directions future research exploring “next one” Eukaryote relative, deciphering function eukaryotic signature proteins eukaryogenesis from both genomic physiological aspects, understanding roles horizontal gene transfer, viruses, mobile elements eukaryogenesis.

Язык: Английский

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Methanotrophic Methanoperedens archaea host diverse and interacting extrachromosomal elements

Nature Microbiology, Год журнала: 2024, Номер 9(9), С. 2422 - 2433

Опубликована: Июнь 25, 2024

Язык: Английский

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Complete genomes of Asgard archaea reveal diverse integrated and mobile genetic elements

Genome Research, Год журнала: 2024, Номер 34(10), С. 1595 - 1609

Опубликована: Окт. 1, 2024

Asgard archaea are of great interest as the progenitors Eukaryotes, but little is known about mobile genetic elements (MGEs) that may shape their ongoing evolution. Here, we describe MGEs replicate in Atabeyarchaeia, a wetland lineage represented by two complete genomes. We used soil depth-resolved population metagenomic data sets to track 18 for which genome structures were defined and precise chromosome integration sites could be identified confident host linkage. Additionally, 20.67 kbp circular plasmid family-level groups viruses linked via CRISPR spacer targeting. Closely related 40 possess hypervariable genomic region encoding combinations specific genes small cysteine-rich proteins structurally similar restriction-homing endonucleases. One 10.9 integrative conjugative element (ICE) integrates genomically into

Язык: Английский

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