bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 20, 2023
Abstract
Candidatus
Altiarchaea
are
widespread
across
aquatic
subsurface
ecosystems
and
possess
a
highly
conserved
core
genome,
yet
adaptations
of
this
genome
to
different
biotic
abiotic
factors
based
on
gene
expression
remain
unknown.
Here,
we
investigated
the
metatranscriptome
two
Ca
.
Altiarchaeum
populations
that
thrive
in
substantially
ecosystems.
In
Crystal
Geyser,
high-CO
2
groundwater
system
USA,
crystalense
co-occurs
with
symbiont
Huberiarchaeum
crystalense,
while
Muehlbacher
sulfidic
spring
Germany,
an
artesian
high
sulfide
concentration,
A.
hamiconexum
is
heavily
infected
viruses.
We
here
mapped
reads
against
their
genomes
analyze
situ
profile
genomes.
Out
537
shared
clusters,
331
were
functionally
annotated
130
differed
significantly
between
sites.
Main
differences
related
genes
involved
cell
defense
like
CRISPR-Cas,
virus
defense,
replication,
transcription
as
well
energy
carbon
metabolism.
Our
results
demonstrate
altiarchaeal
likely
adapted
environment
influenced
by
other
biological
entities
tamper
consequently
posit
viruses
symbiotic
interactions
can
be
major
sinks
for
organisms
deep
biosphere.
(Originality-Significance
Statement
Organisms
uncultivated
phylum
Altiarchaeota
globally
fulfill
essential
roles
cycling,
e
g
.,
fixation
continental
subsurface.
show
transcriptional
activity
differ
depending
geological
microbial
setting
ecosystem
explaining
many
previously
observed
physiological
traits
organism
group.)
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,
Год журнала:
2024,
Номер
74(1)
Опубликована: Янв. 22, 2024
The
International
Code
of
Nomenclature
Prokaryotes
(ICNP)
now
includes
the
categories
domain
and
kingdom.
For
purpose
valid
publication
their
names
under
ICNP,
we
consider
here
two
known
domains,
‘
Bacteria
’
Archaea
’,
as
well
a
number
taxa
suitable
for
rank
kingdom,
based
on
previous
phylogenetic
taxonomic
studies.
It
is
proposed
to
subdivide
into
kingdoms
Bacillati
,
Fusobacteriati
Pseudomonadati
Thermotogati
.
This
arrangement
reflects
contemporary
hypotheses
proposals
cell
wall
structure,
including
‘diderms’
vs.
‘monoderms’,
Gracilicutes
Firmicutes
Negibacteria
Unibacteria
Hydrobacteria
Terrabacteria
Hydrobacterida
Terrabacterida
’.
include
Methanobacteriati
Nanobdellati
Thermoproteati
reflecting
division
Euryarchaeota
‘DPANN
superphylum’
‘TACK
superphylum’.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 16, 2024
Abstract
DPANN
is
a
widespread
and
diverse
group
of
archaea
characterized
by
their
small
size,
reduced
genome,
limited
metabolic
pathways,
symbiotic
existence.
Known
species
are
predominantly
obligate
ectosymbionts
that
depend
on
host
for
proliferation.
The
structural
molecular
details
recognition,
host-DPANN
intercellular
communication,
adaptation
in
response
to
attachment
remain
unknown.
Here,
we
use
electron
cryotomography
(cryo-ET)
show
the
Microcaldus
variisymbioticus
ARM-1
may
interact
with
its
host,
Metallosphaera
javensis
AS-7
through
proteinaceous
nanotubes.
Combining
cryo-ET
sub-tomogram
averaging,
situ
architectures
S-layers
structures
nanotubes
primed
extended
states.
In
addition,
comparative
proteomics
genomic
analyses
identified
proteomic
changes
attachment.
These
results
provide
insights
into
basis
communication
deepen
our
understanding
ectosymbiotic
relationships.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 21, 2024
Abstract
DPANN
is
a
widespread
and
highly
diverse
group
of
archaea
characterised
by
their
small
size,
reduced
genome,
limited
metabolic
pathways,
symbiotic
existence.
Known
species
are
predominantly
obligate
ectosymbionts
that
depend
on
host
for
survival
proliferation.
Despite
the
recent
expansion
in
this
clade,
structural
molecular
details
recognition,
host-DPANN
intercellular
communication,
adaptation
response
to
attachment
remain
unknown.
Here,
we
used
electron
cryotomography
(cryo-ET)
reveal
Candidatus
Micrarchaeota
(ARM-1)
interacts
with
its
host,
Metallosphaera
javensis
through
proteinaceous
nanotubes.
These
tubes
(∼4.5
nm
wide)
originate
extend
all
way
cytoplasm
act
like
tunnels
exchange.
Combining
cryo-ET
sub-tomogram
averaging,
revealed
situ
architectures
S-layers
structures
nanotubes
primed
extended
states,
providing
mechanistic
insights
into
substrate
Additionally,
performed
comparative
proteomics
genomic
analyses
identify
proteomic
changes
attachment.
Our
results
showed
striking
alterations
host-proteome
during
symbiosis
upregulation/downregulation
key
cellular
pathways.
Collectively,
these
provided
unprecedented
basis
communication
deepen
our
understanding
ectosymbiotic
relationships.
Ecosystems
subject
to
mantle
degassing
are
of
particular
interest
for
understanding
global
biogeochemistry,
as
their
microbiomes
shaped
by
prolonged
exposure
high
CO
