Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 15, 2024
Organ
on
Chip
platforms
hold
significant
promise
as
alternatives
to
animal
models
or
traditional
cell
cultures,
both
of
which
poorly
recapitulate
human
pathophysiology
and
level
responses.
Within
the
last
15
years,
we
have
witnessed
seminal
scientific
developments
from
academic
laboratories,
a
flurry
startups
investments,
genuine
interest
pharmaceutical
industry
well
regulatory
authorities
translate
these
platforms.
This
Perspective
identifies
several
fundamental
design
process
features
that
may
act
roadblocks
prevent
widespread
dissemination
deployment
systems,
provides
roadmap
help
position
this
technology
in
mainstream
drug
discovery.
ACS Nano,
Год журнала:
2024,
Номер
18(45), С. 31569 - 31585
Опубликована: Ноя. 1, 2024
Exposure
to
micro-
and
nanoplastics
(MNPs)
has
been
implicated
in
potential
cardiotoxicity.
However,
vitro
models
based
on
cardiomyocyte
cell
lines
lack
crucial
cardiac
characteristics,
while
interspecies
differences
animal
compromise
the
reliability
of
conclusions.
In
addition,
current
research
predominantly
focused
single-time
point
exposures
MNPs,
neglecting
comparative
analyses
injury
across
early
late
stages.
Moreover,
there
remains
a
large
gap
understanding
susceptibility
MNPs
under
pathological
conditions.
To
address
these
limitations,
this
study
integrated
organoids
(COs)
organ-on-a-chip
(OoC)
technology
develop
organoid-on-a-chip
(COoC),
which
was
validated
for
cardiotoxicity
evaluation
through
multiple
dimensions.
Based
COoC,
we
conducted
dynamic
observation
damage
caused
by
short-
long-term
exposure
polystyrene
(PS-NPs).
Oxidative
stress,
inflammation,
disruption
calcium
ion
homeostasis,
mitochondrial
dysfunction
were
confirmed
as
mechanisms
PS-NP-induced
events
stages,
fibrosis
emerged
prominent
feature
Notably,
low-dose
exacerbated
myocardial
infarction
symptoms
states,
despite
no
significant
shown
healthy
models.
conclusion,
findings
further
deepened
our
cardiotoxic
effects
introduced
promising
platform
assessing
Improper
use
of
antibiotics
has
led
to
the
development
antimicrobial
resistance,
or
"superbugs,"
outpacing
discovery
new
antibiotics.
The
lack
rapid,
high-throughput
screening
methods
is
a
major
bottleneck
in
novel
Traditional
consume
significant
amounts
samples,
making
it
challenging
discover
from
limited
natural
product
extracts.
Here,
method
reported
for
products
with
activity
enabled
by
nanoliter
matrix
SlipChip
(nm-SlipChip).
This
nm-SlipChip
creates
droplets
100
drug
candidate-bacterium
combinations.
effectiveness
candidate
assessed
analyzing
microbial
phenotypic
changes.
reduces
sample
consumption
over
5000-fold
and
shortens
detection
time
three
hours.
Twenty
compounds
isolated
Callicarpa
integerrima
were
tested
against
10
pathogenic
bacteria
identified
two
previously
unreported
clerodane
diterpenes
methicillin-resistant
Staphylococcus
aureus
(MRSA).
Molecular
docking
fluorescence
probe
experiments
reveals
that
their
effect
results
disruption
bacterial
cell
membranes
biofilms.
provides
an
effective
discovering
drugs
sources,
vital
combating
antibiotic
resistance.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 7, 2025
Advances
in
tissue
engineering
and
microfluidic
technologies
have
enabled
the
development
of
sophisticated
vitro
models
known
as
organ-on-a-chip
(OoC)
or
microphysiological
systems.
These
systems
enable
to
potential
simulate
dynamic
interactions
between
host
tissues
their
microenvironment
including
microbes,
biomaterials,
mechanical
forces,
pharmaceutical,
consumer-care
products.
fluidic
are
increasingly
being
utilized
investigate
host-microbe
host-material
oral
health
disease.
Of
interest
is
application
understanding
periodontal
disease,
a
chronic
inflammatory
condition
marked
by
progressive
destruction
tissues,
gingiva,
ligament,
alveolar
bone.
The
pathogenesis
disease
involves
complex
interplay
microbial
dysbiosis
immune
responses,
which
can
lead
loss
dental
support
structures
contribute
systemic
conditions
such
cardiovascular
diabetes,
bowel
This
provides
comprehensive
overview
latest
developments
millifluidic
designed
emulate
interactions.
We
discuss
critical
biological
considerations
designing
these
platforms,
applications
studying
biofilms,
unravel
mechanisms
therapeutic
targets
Organs-on-chips
(OoCs)
can
be
directly
fabricated
by
3D
bioprinting
techniques,
which
enhance
the
structural
and
functional
fidelity
of
organ
models
broaden
applications
OoCs.
The EMBO Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 21, 2025
As
the
antimicrobial
resistance
crisis
becomes
a
reality,
we
are
only
now
realizing
lack
fundamental
understanding
of
mechanisms
by
which
major
pathogens
infect
their
human
host.
To
address
this,
must
employ
novel
methodologies
that
allow
us
to
investigate
microbial
behavior
in
infection-like
contexts.
Here,
present
framework
for
studying
host-microbe
interactions
integrating
human-derived
organoids
with
high-resolution
live
imaging
and
high-throughput
sequencing
technologies.
This
interdisciplinary
multiscale
strategy
has
potential
resolve
great
detail
under
conditions
replicating
those
encountered
vivo.
Thus,
infection
studies
based
on
organoid
models
could
open
new
avenues
translational
research
therapeutic
potential.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 12, 2025
Abstract
Lyme
disease,
the
most
widespread
tick‐borne
disease
in
North
America,
is
caused
by
bacterium
Borrelia
burgdorferi
(
Bb
).
Approximately
10–15%
of
infections
result
neuroborreliosis,
common
symptoms
which
include
headaches,
facial
palsy,
and
long‐term
cognitive
impairment.
Previous
studies
dissemination
focus
on
assessing
transmigration
at
static
time
points
rather
than
analyzing
complex
dynamic
process
extravasation.
Furthermore,
current
vitro
models
lack
crucial
physiological
factors
such
as
flow,
demonstrating
a
need
for
more
robust
studying
to
understand
its
dynamics
mechanisms.
Here,
3D
tissue‐engineered
microvessel
model
used
fluorescently‐labeled
perfused
vascular
non‐tissue‐specific
(iEC)
brain‐specific
(iBMEC)
microvessels
while
acquiring
time‐lapse
images
real
time.
In
iECs,
extravasation
involves
two
steps:
adhesion
endothelium
into
extracellular
matrix,
can
be
modulated
through
glycocalyx
degradation
or
inflammation.
contrast,
iBMECs
an
extremely
rare
event
regardless
addition,
circulating
do
not
induce
endothelial
activation
iECs
iBMECs,
but
induces
barrier
dysfunction
iECs.
These
findings
provide
further
understanding
dissemination.
