Bioactive Materials,
Год журнала:
2021,
Номер
14, С. 169 - 181
Опубликована: Дек. 17, 2021
Nanosized
extracellular
vesicles
derived
from
bacteria
contain
diverse
cargo
and
transfer
intercellular
bioactive
molecules
to
cells.
Due
their
favorable
interactions,
cell
membrane-derived
bacterial
(BEVs)
have
great
potential
become
novel
drug
delivery
platforms.
In
this
review,
we
summarize
the
biogenesis
mechanism
compositions
of
various
BEVs.
addition,
an
overview
effective
isolation
purification
techniques
BEVs
is
provided.
particular,
focus
on
application
as
nanocarriers
for
delivery.
Finally,
advances
challenges
after
providing
a
comprehensive
discussion
in
each
section.
We
believe
that
deeper
understanding
will
open
new
avenues
exploitation
applications.
Journal of Extracellular Vesicles,
Год журнала:
2024,
Номер
13(2)
Опубликована: Фев. 1, 2024
Extracellular
vesicles
(EVs),
through
their
complex
cargo,
can
reflect
the
state
of
cell
origin
and
change
functions
phenotypes
other
cells.
These
features
indicate
strong
biomarker
therapeutic
potential
have
generated
broad
interest,
as
evidenced
by
steady
year-on-year
increase
in
numbers
scientific
publications
about
EVs.
Important
advances
been
made
EV
metrology
understanding
applying
biology.
However,
hurdles
remain
to
realising
EVs
domains
ranging
from
basic
biology
clinical
applications
due
challenges
nomenclature,
separation
non-vesicular
extracellular
particles,
characterisation
functional
studies.
To
address
opportunities
this
rapidly
evolving
field,
International
Society
for
Vesicles
(ISEV)
updates
its
'Minimal
Information
Studies
Vesicles',
which
was
first
published
2014
then
2018
MISEV2014
MISEV2018,
respectively.
The
goal
current
document,
MISEV2023,
is
provide
researchers
with
an
updated
snapshot
available
approaches
advantages
limitations
production,
multiple
sources,
including
culture,
body
fluids
solid
tissues.
In
addition
presenting
latest
art
principles
research,
document
also
covers
advanced
techniques
that
are
currently
expanding
boundaries
field.
MISEV2023
includes
new
sections
on
release
uptake
a
brief
discussion
vivo
study
Compiling
feedback
ISEV
expert
task
forces
more
than
1000
researchers,
conveys
research
facilitate
robust
discoveries
move
field
forward
even
rapidly.
Signal Transduction and Targeted Therapy,
Год журнала:
2023,
Номер
8(1)
Опубликована: Май 25, 2023
Abstract
Blood–brain
barrier
(BBB)
is
a
natural
protective
membrane
that
prevents
central
nervous
system
(CNS)
from
toxins
and
pathogens
in
blood.
However,
the
presence
of
BBB
complicates
pharmacotherapy
for
CNS
disorders
as
most
chemical
drugs
biopharmaceuticals
have
been
impeded
to
enter
brain.
Insufficient
drug
delivery
into
brain
leads
low
therapeutic
efficacy
well
aggravated
side
effects
due
accumulation
other
organs
tissues.
Recent
breakthrough
materials
science
nanotechnology
provides
library
advanced
with
customized
structure
property
serving
powerful
toolkit
targeted
delivery.
In-depth
research
field
anatomical
pathological
study
on
further
facilitates
development
brain-targeted
strategies
enhanced
crossing.
In
this
review,
physiological
different
cells
contributing
are
summarized.
Various
emerging
permeability
regulation
crossing
including
passive
transcytosis,
intranasal
administration,
ligands
conjugation,
coating,
stimuli-triggered
disruption,
overcome
obstacle
highlighted.
Versatile
systems
ranging
organic,
inorganic,
biologics-derived
their
synthesis
procedures
unique
physio-chemical
properties
summarized
analyzed.
This
review
aims
provide
an
up-to-date
comprehensive
guideline
researchers
diverse
fields,
offering
perspectives
system.
Trends in Cell Biology,
Год журнала:
2023,
Номер
33(8), С. 667 - 681
Опубликована: Фев. 1, 2023
The
study
of
extracellular
vesicles
(EVs)
and
nanoparticles
(NPs)
is
rapidly
expanding
because
recent
discoveries
have
revealed
a
much
greater
complexity
diversity
than
was
appreciated
only
few
years
ago.
New
types
EVs
NPs
recently
been
described.
Proteins
nucleic
acids
previously
thought
to
be
packaged
in
exosomes
appear
more
enriched
different
two
identified
amembranous
NPs,
exomeres
supermeres.
Thus,
our
understanding
the
cell
biology
intercellular
communication
facilitated
by
release
state
flux.
In
this
review,
we
describe
highlight
advances,
present
major
outstanding
questions.
Cell Death and Disease,
Год журнала:
2022,
Номер
13(7)
Опубликована: Июль 4, 2022
Mesenchymal
stem
cells
(MSCs)
can
be
widely
isolated
from
various
tissues
including
bone
marrow,
umbilical
cord,
and
adipose
tissue,
with
the
potential
for
self-renewal
multipotent
differentiation.
There
is
compelling
evidence
that
therapeutic
effect
of
MSCs
mainly
depends
on
their
paracrine
action.
Extracellular
vesicles
(EVs)
are
fundamental
effectors
play
a
crucial
role
in
intercellular
communication,
existing
body
fluids
cell
supernatants.
Since
MSC-derived
EVs
retain
function
protocells
have
lower
immunogenicity,
they
wide
range
prospective
applications
advantages
over
therapy.
We
describe
some
characteristics
MSC-EVs,
discuss
immune
regulation
regeneration,
emphasis
molecular
mechanism
application
MSC-EVs
treatment
fibrosis
support
tissue
repair.
also
highlight
current
challenges
clinical
ways
to
overcome
problem
quality
heterogeneity.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Фев. 5, 2024
Abstract
Extracellular
vesicles
(EVs)
are
nano-sized,
membranous
structures
secreted
into
the
extracellular
space.
They
exhibit
diverse
sizes,
contents,
and
surface
markers
ubiquitously
released
from
cells
under
normal
pathological
conditions.
Human
serum
is
a
rich
source
of
these
EVs,
though
their
isolation
proteins
non-EV
lipid
particles
poses
challenges.
These
transport
various
cellular
components
such
as
proteins,
mRNAs,
miRNAs,
DNA,
lipids
across
distances,
influencing
numerous
physiological
events,
including
those
within
tumor
microenvironment
(TME).
Their
pivotal
roles
in
communication
make
EVs
promising
candidates
for
therapeutic
agents,
drug
delivery
systems,
disease
biomarkers.
Especially
cancer
diagnostics,
EV
detection
can
pave
way
early
identification
offers
potential
diagnostic
Moreover,
subtypes
emerging
targeted
tools,
highlighting
clinical
significance.
The
need
non-invasive
biomarkers
to
monitor
biological
processes
purposes
remains
unfulfilled.
Tapping
unique
composition
could
unlock
advanced
avenues
future.
In
this
review,
we
discuss
detail
conditions,
cancers
(encompassing
head
neck,
lung,
gastric,
breast,
hepatocellular
carcinoma),
neurodegenerative
disorders,
diabetes,
viral
infections,
autoimmune
renal
diseases,
emphasizing
advancements
molecular
diagnostics
delivery.
Signal Transduction and Targeted Therapy,
Год журнала:
2023,
Номер
8(1)
Опубликована: Март 17, 2023
Abstract
Chronic
kidney
disease
(CKD)
is
estimated
to
affect
10–14%
of
global
population.
Kidney
fibrosis,
characterized
by
excessive
extracellular
matrix
deposition
leading
scarring,
a
hallmark
manifestation
in
different
progressive
CKD;
However,
at
present
no
antifibrotic
therapies
against
CKD
exist.
fibrosis
identified
tubule
atrophy,
interstitial
chronic
inflammation
and
fibrogenesis,
glomerulosclerosis,
vascular
rarefaction.
Fibrotic
niche,
where
organ
initiates,
complex
interplay
between
injured
parenchyma
(like
tubular
cells)
multiple
non-parenchymal
cell
lineages
(immune
mesenchymal
located
spatially
within
scarring
areas.
Although
the
mechanisms
are
complicated
due
kinds
cells
involved,
with
help
single-cell
technology,
many
key
questions
have
been
explored,
such
as
what
kind
renal
tubules
profibrotic,
myofibroblasts
originate,
which
immune
how
communicate
each
other.
In
addition,
genetics
epigenetics
deeper
that
regulate
fibrosis.
And
reversible
nature
epigenetic
changes
including
DNA
methylation,
RNA
interference,
chromatin
remodeling,
gives
an
opportunity
stop
or
reverse
therapeutic
strategies.
More
marketed
(e.g.,
RAS
blockage,
SGLT2
inhibitors)
developed
delay
progression
recent
years.
Furthermore,
better
understanding
also
favored
discover
biomarkers
fibrotic
injury.
review,
we
update
advances
mechanism
summarize
novel
treatment
for
CKD.
Journal of Hematology & Oncology,
Год журнала:
2021,
Номер
14(1)
Опубликована: Сен. 3, 2021
Abstract
Extracellular
vesicles
(EVs)
are
cell-derived
membrane
structures
enclosing
proteins,
lipids,
RNAs,
metabolites,
growth
factors,
and
cytokines.
EVs
have
emerged
as
essential
intercellular
communication
regulators
in
multiple
physiological
pathological
processes.
Previous
studies
revealed
that
mesenchymal
stem
cells
(MSCs)
could
either
support
or
suppress
tumor
progression
different
cancers
by
paracrine
signaling
via
MSC-derived
EVs.
Evidence
suggested
mimic
their
parental
cells,
possessing
pro-tumor
anti-tumor
effects,
inherent
tropism.
Therefore,
can
be
a
cell-free
cancer
treatment
alternative.
This
review
discusses
insights
regarding
EVs'
roles
summarizes
bioengineered
EVs’
applications
safe
versatile
agent
delivery
platforms.
Meanwhile,
current
hurdles
of
moving
from
bench
to
bedside
also
discussed.
