Disulfide-bonded
peptides
and
proteins,
including
hormones,
toxins,
growth
factors,
others,
are
abundant
in
living
organisms.
These
molecules
play
crucial
physiological
roles
such
as
regulating
cell
organism
growth,
development,
metabolism.
They
have
also
found
widespread
applications
drugs
or
tool
biomedical
pharmaceutical
research.
However,
the
chemical
synthesis
of
disulfide-bonded
proteins
is
complicated
by
challenges
associated
with
their
folding.
This
review
focuses
on
latest
advancements
peptide
protein
folding
technologies.
Particularly,
it
highlights
biomimetic
strategies
that
emulate
naturally
occurring
oxidative
processes
nature.
include
chaperone-assisted
folding,
glycosylation-assisted
organic-based
methods.
The
anticipates
future
directions
technology.
Such
research
offers
innovative
approaches
for
complex
otherwise
difficult
to
fold.
Organic & Biomolecular Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
A
mild
and
efficient
FeCl
3
-catalyzed
oxidative
diselenylation
of
pyrrole-tethered
indoles
has
been
achieved
by
using
RSeSeR
as
the
source
selenium
m
-chloroperoxybenzoic
acid
(
CPBA)
oxidant
(yields
17–70%).
International Journal of Biological Sciences,
Год журнала:
2024,
Номер
20(7), С. 2779 - 2789
Опубликована: Янв. 1, 2024
Selenium
(Se)
is
an
essential
trace
element
for
biological
processes.Seleno-amino
acids
(Se-AAs),
known
as
the
organic
forms
of
Se,
and
their
metabolic
reprogramming
have
been
increasingly
recognized
to
regulate
antioxidant
defense,
enzyme
activity,
tumorigenesis.Therefore,
there
emerging
interest
in
exploring
potential
application
Se-AAs
antitumor
therapy.In
addition
playing
a
vital
role
inhibiting
tumor
growth,
accumulating
evidence
has
revealed
that
Se-AA
metabolism
could
reshape
microenvironment
(TME)
enhance
immunotherapy
responses.This
review
presents
comprehensive
overview
current
progress
multifunctional
treatment,
with
particular
emphasis
on
elucidating
crosstalk
between
various
cell
types
TME,
including
cells,
T
macrophages,
natural
killer
cells.Furthermore,
novel
applications
integrating
are
also
discussed
alongside
prospects
provide
new
insights
into
this
field.
Bioconjugate Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 5, 2025
Post-translational
modifications
(PTMs)
on
histones
play
a
crucial
role
in
determining
the
structure
and
function
of
chromatin,
thereby
regulating
eukaryotic
gene
expression.
Histone
lysine
methylation
acetylation
are
among
most
widespread
biomedically
important
PTMs,
with
new
chemical
tools
for
their
examination
high
demand.
Here,
we
report
first
use
γ-selenalysine
as
an
efficient
mimic
enzymatic
methylation,
acetylation,
deacetylation
reactions
catalyzed
by
histone
methyltransferases,
acetyltransferases,
deacetylase.
We
also
show
that
easily
accessible
selenocysteine
cysteine
residues
can
undergo
chemo-
site-selective
alkylation
to
generate
both
unmodified
modified
related
γ-thialysine
peptides.
This
dual-modification
strategy
enables
site-specific
incorporation
two
distinct
functionalities
into
peptides,
mimicking
post-translational
commonly
found
histones.
Our
research
presents
novel
approach
which
serves
unique
handle
chemoselective
introduction
selenalysine,
along
its
methylated
acetylated
analogues.
These
designed
facilitate
study
epigenetic
proteins
human
health
disease.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(31), С. 7532 - 7542
Опубликована: Янв. 1, 2024
Hydrophilic
peptides
(HPs)
play
a
critical
role
in
the
pathogenesis
of
hepatocellular
carcinoma
(HCC).
However,
comprehensive
and
in-depth
high-throughput
analysis
specific
changes
HPs
associated
with
HCC
remains
unrealized,
due
to
complex
nature
biological
fluids
challenges
mining
patterns
large
data
sets.
The
clinical
diagnosis
still
lacks
non-destructive
accurate
classification
method,
given
limited
specificity
widely
used
biomarkers.
To
address
these
challenges,
we
have
established
multifunctional
platform
that
integrates
artificial
intelligence
computation,
hydrophilic
interaction
extraction
HPs,
MALDI-MS
testing.
This
aims
achieve
highly
sensitive
HP
fingerprinting
for
HCC.
method
not
only
facilitates
efficient
detection
but
also
achieves
remarkable
100.00%
diagnostic
accuracy
test
cohort,
supported
by
machine
learning
algorithms.
By
constructing
panel
10
characteristic
features,
achieved
98%
cohort
rapid
identified
62
deeply
involved
pathways
related
liver
diseases.
integrated
strategy
provides
new
research
directions
future
biomarker
studies
as
well
early
individualized
treatment
Angewandte Chemie,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 14, 2024
Abstract
Elucidating
the
chemical
structure
and
intracellular
action
mechanisms
is
still
critical
limit
for
clinical
translation
of
nanomedicines.
Intracellular
redox
environments
originating
from
cell
metabolism
are
key
factors
affecting
internalized
drug
efficacy.
Herein,
we
engineer
Se−Se/Se−S
bond
to
assemble
selenium
(Se)
nanoclusters
(SeClus)
with
environment‐driven
selective
structure.
Chemical
analysis
reveals
that,
bonding
sulfur
atom
in
intermediates
two
neighboring
or
interposition
Se
atoms
rings
internal
driving
force
SeClus
formation.
This
nanocluster
can
be
predominantly
transformed
selenocysteine
facilitate
selenoproteins
synthesis
normal
cells,
while
metabolize
cytotoxic
SeO
3
2−
based
on
oxidative
environment
cancer
cells.
Resultantly,
exhibit
significant
proliferation
inhibition
ability
cells
impressive
safety
Taken
together,
this
study
not
only
clarifies
nature
engineering
SeClus,
but
also
elucidates
its
environment‐oriented
anticancer
mechanism.
Organic Letters,
Год журнала:
2024,
Номер
26(29), С. 6274 - 6278
Опубликована: Июль 15, 2024
A
calcium-mediated
three-component
selenylation
of
gem-difluoroalkenes
using
alcohols
as
nucleophiles
and
N-(phenylseleno)phthalimide
the
agent
has
been
developed
for
efficient
synthesis
various
α,α-difluoroalkyl-β-selenides.
This
reaction
exhibits
broad
substrate
functional
group
tolerance,
along
with
high
levels
chemo-
regioselectivity.
Additionally,
synthetic
utility
transformation
in
late-stage
functionalization
drug
molecules
was
demonstrated.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(49)
Опубликована: Авг. 12, 2024
Abstract
Antisense
oligonucleotide
(ASO)
therapies
hold
significant
promise
in
the
realm
of
molecular
medicine.
By
precisely
targeting
RNA
molecules,
ASOs
offer
an
approach
to
modulate
gene
expression
and
protein
production,
making
them
valuable
tools
for
treating
a
wide
range
genetic
acquired
diseases.
As
precise
intracellular
delivery
is
challenging,
strategies
preparing
ASO‐ligand
conjugates
are
exceedingly
high
demand.
This
work
leverages
utility
native
chemical
ligation
conjugate
with
therapeutically
relevant
modifications
including
locked
nucleic
acids
phosphorothioate
backbone
peptides
sugars
via
stable
amide
linkage.
A
suite
post‐ligation
functionalizations
through
modification
cysteine
handle
highlighted,
chemoselective
radical
desulfurization,
lipidation,
alkylation
handles
(e.g.
alkyne,
biotin,
radionuclide
chelating
ligands),
affording
multifunctional
constructs
further
applications
biology
Application
methodology
clinically‐relevant
triantennary‐GalNAc
ASO
validation
its
binding
functional
activity
underpins
applicability
technique
oligonucleotide‐based
therapeutics.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 14, 2024
Elucidating
the
chemical
structure
and
intracellular
action
mechanisms
is
still
critical
limit
for
clinical
translation
of
nanomedicines.
Intracellular
redox
environments
originating
from
cell
metabolism
are
key
factors
affecting
internalized
drug
efficacy.
Herein,
we
engineer
Se-Se/Se-S
bond
to
assemble
selenium
(Se)
nanoclusters
(SeClus)
with
environment-driven
selective
structure.
Chemical
analysis
reveals
that,
bonding
sulfur
atom
in
intermediates
two
neighboring
or
interposition
Se
atoms
rings
internal
driving
force
SeClus
formation.
This
nanocluster
can
be
predominantly
transformed
selenocysteine
facilitate
selenoproteins
synthesis
normal
cells,
while
metabolize
cytotoxic
SeO
