Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 23, 2024
An
increasing
population,
climate
change,
and
diminishing
natural
resources
present
severe
threats
to
global
food
security,
with
traditional
breeding
genetic
engineering
methods
often
falling
short
in
addressing
these
rapidly
evolving
challenges.
CRISPR/Cas
systems
have
emerged
as
revolutionary
tools
for
precise
modifications
crops,
offering
significant
advancements
resilience,
yield,
nutritional
value,
particularly
staple
crops
like
rice
maize.
This
review
highlights
the
transformative
potential
of
technology,
emphasizing
recent
innovations
such
prime
base
editing,
development
novel
CRISPR-associated
proteins,
which
significantly
improved
specificity,
efficiency,
scope
genome
editing
agriculture.
These
enable
targeted
that
enhance
tolerance
abiotic
stresses
well
biotic
stresses.
Additionally,
plays
a
crucial
role
improving
crop
yield
quality
by
enhancing
photosynthetic
nutrient
uptake,
resistance
lodging,
while
also
taste,
texture,
shelf
life,
content
through
biofortification.
Despite
challenges
off-target
effects,
need
more
efficient
delivery
methods,
ethical
regulatory
concerns,
underscores
importance
security
sustainability
It
calls
continued
research
integration
CRISPR
other
emerging
technologies
nanotechnology,
synthetic
biology,
machine
learning
fully
realize
its
developing
resilient,
productive,
sustainable
agricultural
systems.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Июль 17, 2024
Traditional
therapeutic
approaches
such
as
chemotherapy
and
radiation
therapy
have
burdened
cancer
patients
with
onerous
physical
psychological
challenges.
Encouragingly,
the
landscape
of
tumor
treatment
has
undergone
a
comprehensive
remarkable
transformation.
Emerging
fervently
pursued
modalities
are
small
molecule
targeted
agents,
antibody-drug
conjugates
(ADCs),
cell-based
therapies,
gene
therapy.
These
cutting-edge
not
only
afford
personalized
precise
targeting,
but
also
provide
enhanced
comfort
potential
to
impede
disease
progression.
Nonetheless,
it
is
acknowledged
that
these
strategies
still
harbour
untapped
for
further
advancement.
Gaining
understanding
merits
limitations
holds
promise
offering
novel
perspectives
clinical
practice
foundational
research
endeavours.
In
this
review,
we
discussed
different
modalities,
including
drugs,
peptide
antibody
cell
therapy,
It
will
detailed
explanation
each
method,
addressing
their
status
development,
challenges,
solutions.
The
aim
assist
clinicians
researchers
in
gaining
deeper
diverse
options,
enabling
them
carry
out
effective
advance
more
efficiently.
Advanced Materials,
Год журнала:
2023,
Номер
35(51)
Опубликована: Май 17, 2023
Abstract
Messenger
RNA
(mRNA)
has
received
great
attention
in
the
prevention
and
treatment
of
various
diseases
due
to
success
coronavirus
disease
2019
(COVID‐19)
mRNA
vaccines
(Comirnaty
Spikevax).
To
meet
therapeutic
purpose,
it
is
required
that
must
enter
target
cells
express
sufficient
proteins.
Therefore,
development
effective
delivery
systems
necessary
crucial.
Lipid
nanoparticle
(LNP)
represents
a
remarkable
vehicle
indeed
accelerated
applications
humans,
as
several
mRNA‐based
therapies
have
already
been
approved
or
are
clinical
trials.
In
this
review,
focus
on
mRNA‐LNP‐mediated
anticancer
therapy.
It
summarizes
main
strategies
mRNA‐LNP
formulations,
discusses
representative
approaches
cancer,
points
out
current
challenges
possible
future
directions
research
field.
hoped
these
delivered
messages
can
help
further
improve
application
technology
cancer
Cell,
Год журнала:
2024,
Номер
187(5), С. 1076 - 1100
Опубликована: Фев. 1, 2024
Genome
editing
has
been
a
transformative
force
in
the
life
sciences
and
human
medicine,
offering
unprecedented
opportunities
to
dissect
complex
biological
processes
treat
underlying
causes
of
many
genetic
diseases.
CRISPR-based
technologies,
with
their
remarkable
efficiency
easy
programmability,
stand
at
forefront
this
revolution.
In
Review,
we
discuss
current
state
CRISPR
gene
technologies
both
research
therapy,
highlighting
limitations
that
constrain
them
technological
innovations
have
developed
recent
years
address
them.
Additionally,
examine
summarize
landscape
applications
context
health
therapeutics.
Finally,
outline
potential
future
developments
could
shape
coming
years.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Янв. 13, 2023
CRISPR-Cas
gene
editing
has
revolutionized
experimental
molecular
biology
over
the
past
decade
and
holds
great
promise
for
treatment
of
human
genetic
diseases.
Here
we
review
development
CRISPR-Cas9/Cas12/Cas13
nucleases,
DNA
base
editors,
prime
RNA
focusing
on
assessment
improvement
their
precision
safety,
pushing
limit
specificity
efficiency.
We
summarize
capabilities
limitations
each
CRISPR
tool
from
to
editing,
highlight
opportunities
future
improvements
applications
in
basic
research,
as
well
therapeutic
clinical
considerations
use
patients.
Nature Biomedical Engineering,
Год журнала:
2023,
Номер
7(5), С. 616 - 628
Опубликована: Апрель 17, 2023
Sickle-cell
disease
(SCD)
is
caused
by
an
A·T-to-T·A
transversion
mutation
in
the
β-globin
gene
(HBB).
Here
we
show
that
prime
editing
can
correct
SCD
allele
(HBB
Nature,
Год журнала:
2024,
Номер
628(8008), С. 639 - 647
Опубликована: Апрель 3, 2024
Abstract
Prime
editing
enables
the
precise
modification
of
genomes
through
reverse
transcription
template
sequences
appended
to
3′
ends
CRISPR–Cas
guide
RNAs
1
.
To
identify
cellular
determinants
prime
editing,
we
developed
scalable
reporters
and
performed
genome-scale
CRISPR-interference
screens.
From
these
screens,
a
single
factor
emerged
as
strongest
mediator
editing:
small
RNA-binding
exonuclease
protection
La.
Further
investigation
revealed
that
La
promotes
across
approaches
(PE2,
PE3,
PE4
PE5),
edit
types
(substitutions,
insertions
deletions),
endogenous
loci
cell
but
has
no
consistent
effect
on
genome-editing
rely
standard,
unextended
RNAs.
Previous
work
shown
binds
polyuridine
tracts
at
RNA
polymerase
III
transcripts
2
We
found
functionally
interacts
with
polyuridylated
(pegRNAs).
Guided
by
results,
editor
protein
(PE7)
fused
RNA-binding,
N-terminal
domain
This
improved
expressed
pegRNAs
engineered
(epegRNAs),
well
synthetic
optimized
for
binding.
Together,
our
results
provide
key
insights
into
how
components
interact
environment
suggest
general
strategies
stabilizing
exogenous
therein.
