Material and microbial perspectives on understanding the role of biochar in mitigating ammonia inhibition during anaerobic digestion

Water Research, Год журнала: 2024, Номер 255, С. 121503 - 121503

Опубликована: Март 22, 2024

Язык: Английский

---

Isolation of a methyl-reducing methanogen outside the Euryarchaeota

Nature, Год журнала: 2024, Номер 632(8027), С. 1124 - 1130

Опубликована: Июль 24, 2024

Язык: Английский

---

Two-Stage Anaerobic Digestion for Green Energy Production: A Review

Processes, Год журнала: 2025, Номер 13(2), С. 294 - 294

Опубликована: Янв. 21, 2025

Anaerobic digestion (AD) is a biotechnological process in which the microorganisms degrade complex organic matter to simpler components under anaerobic conditions produce biogas and fertilizer. This has many environmental benefits, such as green energy production, waste treatment, protection, greenhouse gas emissions reduction. It long been known that two main species (acidogenic bacteria methanogenic archaea) community of AD differ aspects, optimal for their growth development are different. Therefore, if performed single bioreactor (single-phase process), selected taking into account slow-growing methanogens at expense fast-growing acidogens, affecting efficiency whole process. led two-stage (TSAD) recent years, where processes divided cascade separate bioreactors (BRs). division consecutive BRs leads significantly higher yields two-phase system (H2 + CH4) compared traditional single-stage CH4 production review presents state art, advantages disadvantages, some perspectives (based on more than 210 references from 2002 2024 our own studies), including all aspects TSAD—different parameters’ influences, types bioreactors, microbiology, mathematical modeling, automatic control, energetical considerations TSAD processes.

Язык: Английский

---

Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes

Biochemistry, Год журнала: 2019, Номер 58(52), С. 5198 - 5220

Опубликована: Апрель 5, 2019

Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and reversible reduction of methyl-coenzyme (CH3–S-CoM) with B (HS-CoM) to methane CoM-S–S-CoB. MCR is also involved anaerobic oxidation reverse methanogenesis most probably ethane, propane, butane. challenging question how unreactive CH3–S thioether bond even more C–H other hydrocarbons are anaerobically cleaved. A key answer negative redox potential (Eo′) Ni(II)F-430/Ni(I)F-430 couple below −600 mV radical nature Ni(I)F-430. However, one-electron Achilles heel MCR; it makes nickel one O2-sensitive enzymes known date. Even under physiological conditions, Ni(I) oxidized Ni(II) or Ni(III) states, e.g., when cells (E′) CoM-S–S-CoB/HS-CoM HS-CoB (Eo′ = −140 mV) gets too high. Methanogens therefore harbor an system for reactivation inactivated ATP-dependent reaction. Purification state very has been achieved only few laboratories. This perspective reviews function, structure, properties MCR, what not about catalytic mechanism, inactive reactivated, remains be discovered.

Язык: Английский

---

Insights into the ecological roles and evolution of methyl-coenzyme M reductase-containing hot spring Archaea

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Окт. 8, 2019

Abstract Several recent studies have shown the presence of genes for key enzyme associated with archaeal methane/alkane metabolism, methyl-coenzyme M reductase (Mcr), in metagenome-assembled genomes (MAGs) divergent to existing lineages. Here, we study mcr -containing MAGs from several hot springs, which reveal further expansion diversity organisms performing metabolism. Significantly, an MAG basal phylum Thaumarchaeota that contains genes, but not those ammonia oxidation or aerobic is identified. Together, our phylogenetic analyses and ancestral state reconstructions suggest a mostly vertical evolution mcrABG among methanogens methanotrophs, along frequent horizontal gene transfer between alkanotrophs. Analysis all MAGs/genomes suggests hydrothermal origin these microorganisms based on optimal growth temperature predictions. These results also methanogenesis at high likely existed common ancestor.

Язык: Английский

---

Structure, function, and biosynthesis of nickel‐dependent enzymes

Protein Science, Год журнала: 2020, Номер 29(5), С. 1071 - 1089

Опубликована: Фев. 5, 2020

Abstract Nickel enzymes, present in archaea, bacteria, plants, and primitive eukaryotes are divided into redox nonredox enzymes play key functions diverse metabolic processes, such as energy metabolism virulence. They catalyze various reactions by using active sites of complexities, mononuclear nickel Ni‐superoxide dismutase, glyoxylase I acireductone dioxygenase, dinuclear urease, heteronuclear metalloclusters [NiFe]‐carbon monoxide dehydrogenase, acetyl‐CoA decarbonylase/synthase [NiFe]‐hydrogenase, even more complex cofactors methyl‐CoM reductase lactate racemase. The presence metalloenzymes a cell necessitates tight regulation metal homeostasis, order to maintain the appropriate intracellular concentration while avoiding its toxicity. As well, biosynthesis insertion often require specific elaborated maturation pathways, allowing correct be delivered incorporated target enzyme. In this review, phylogenetic distribution will briefly described. Their tridimensional structures well complexity their discussed. view latest findings on these special focus put activation apo‐enzymes.

Язык: Английский

---

Role of redox-active biochar with distinctive electrochemical properties to promote methane production in anaerobic digestion of waste activated sludge

Journal of Cleaner Production, Год журнала: 2020, Номер 278, С. 123212 - 123212

Опубликована: Авг. 7, 2020

Язык: Английский

---

Inhibitors of the methane fermentation process with particular emphasis on the microbiological aspect: A review

Energy Science & Engineering, Год журнала: 2020, Номер 8(5), С. 1880 - 1897

Опубликована: Фев. 11, 2020

Abstract Methane fermentation is an attractive practice in waste processing, which enables one to both control pollution and recover energy. This kind of anaerobic digestion exposed inhibitors, can retard the process cause failure. The mechanism causing toxicity these substances their impact on efficiency are already known, but there still not much information about influence methane microorganisms' activity composition microbiota. In this review, based 168 articles, we present a summary up‐to‐date research inhibition processes by some specific toxicants: ammonia, sulfides, ions light metals, heavy antibiotics, ethylene acetylene, chlorophenols, halogen aliphatic hydrocarbons, nitro compounds, long‐chain fatty acids. review principally focuses inhibitors microorganisms involved process. More accurate recognition mechanisms, with particular emphasis microbiological aspect, help improve

Язык: Английский

---

Thermogenic hydrocarbon biodegradation by diverse depth-stratified microbial populations at a Scotian Basin cold seep

Nature Communications, Год журнала: 2020, Номер 11(1)

Опубликована: Ноя. 17, 2020

At marine cold seeps, gaseous and liquid hydrocarbons migrate from deep subsurface origins to the sediment-water interface. Cold seep sediments are known host taxonomically diverse microorganisms, but little is about their metabolic potential depth distribution in relation hydrocarbon electron acceptor availability. Here we combined geophysical, geochemical, metagenomic metabolomic measurements profile microbial activities at a newly discovered sea. Metagenomic profiling revealed compositional functional differentiation between near-surface deeper layers. In both sulfate-rich sulfate-depleted depths, various archaeal bacterial community members actively oxidizing thermogenic anaerobically. Depth distributions of hydrocarbon-oxidizing archaea that they not necessarily associated with sulfate reduction, which especially surprising for anaerobic ethane butane oxidizers. Overall, these findings link subseafloor microbiomes biochemical mechanisms degradation deeply-sourced hydrocarbons.

Язык: Английский

---

Anthropogenic and Environmental Constraints on the Microbial Methane Cycle in Coastal Sediments

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Фев. 18, 2021

Large amounts of methane, a potent greenhouse gas, are produced in anoxic sediments by methanogenic archaea. Nonetheless, over 90% the methane is oxidized via sulfate-dependent anaerobic oxidation (S-AOM) sulfate-methane transition zone (SMTZ) consortia methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). Coastal systems account for majority total marine emissions typically have lower sulfate concentrations, hence S-AOM less significant. However, alternative electron acceptors such as metal oxides or nitrate could be used AOM instead sulfate. The availability determined redox zonation sediment, which may vary due to changes oxygen type rate organic matter inputs. Additionally, eutrophication climate change can affect microbiome, biogeochemical zonation, cycling coastal sediments. This review summarizes current knowledge on processes microorganisms involved factors influencing from these systems. In eutrophic areas, inputs key driver bottom water hypoxia. Global warming reduce solubility surface waters, enhancing column stratification, increasing primary production, favoring methanogenesis. ANME notoriously slow growers not able effectively oxidize upon rapid sedimentation shoaling SMTZ. settings, ANME-2d ( Methanoperedenaceae ) ANME-2a couple iron- and/or manganese reduction AOM, while NC10 Methylomirabilota nitrite reduction. Ultimately, aerobic methanotrophs upper millimeters sediment column. role mitigating sediments, including exact pathways involved, still underexplored, controlling unclear. Further studies needed order understand driving methane-cycling identify responsible microorganisms. Integration microbial geochemical expected lead more accurate predictions zones future.

Язык: Английский

---