Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMethanosarcina

Microbiology and Molecular Biology Reviews, Год журнала: 2019, Номер 83(4)

Опубликована: Сен. 17, 2019

The biological production of methane is vital to the global carbon cycle and accounts for ca. 74% total emissions. organisms that facilitate this process, methanogenic archaea, belong a large phylogenetically diverse group thrives in wide range anaerobic environments. Two main subgroups exist within archaea: those with without cytochromes. Although variety metabolisms group, reduction growth substrates using electrons from molecular hydrogen is, phylogenetic sense, most widespread pathway. Methanogens cytochromes typically generate by CO2 derived H2, formate, or secondary alcohols, generating transmembrane ion gradient ATP via an Na+-translocating methyltransferase (Mtr). These also conserve energy novel flavin-based electron bifurcation mechanism, wherein endergonic ferredoxin facilitated exergonic disulfide terminal acceptor coupled either H2 formate oxidation. utilize have broader substrate range, can convert acetate methylated compounds methane, addition ability reduce Cytochrome-containing methanogens are able supplement motive force generated Mtr H+-translocating transport system. In both groups, enzymes known as hydrogenases, which reversibly interconvert protons hydrogen, play central role process. This review discusses recent insight into methanogen metabolism conservation mechanisms particular focus on genus Methanosarcina.

Язык: Английский

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Treatment of anthraquinone dye textile wastewater using anaerobic dynamic membrane bioreactor: Performance and microbial dynamics

Chemosphere, Год журнала: 2019, Номер 238, С. 124539 - 124539

Опубликована: Авг. 10, 2019

Язык: Английский

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Insight into the function and evolution of the Wood–Ljungdahl pathway in Actinobacteria

The ISME Journal, Год журнала: 2021, Номер 15(10), С. 3005 - 3018

Опубликована: Май 5, 2021

Язык: Английский

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Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations

Microbiome, Год журнала: 2021, Номер 9(1)

Опубликована: Ноя. 26, 2021

Abstract Background Peatlands are expected to experience sustained yet fluctuating higher temperatures due climate change, leading increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions these processes in peatlands underlain with permafrost, little is known about viruses other peatlands. More generally, soil biogeography its potential drivers poorly understood at both local global scales. Here, 87 metagenomes five size-fraction (viromes) from a boreal peatland northern Minnesota (the SPRUCE whole-ecosystem warming experiment surrounding bog) were analyzed dsDNA community ecological patterns, the recovered populations (vOTUs) compared our curated PIGEON database of 266,125 vOTUs diverse ecosystems. Results Within experiment, composition was significantly correlated peat depth, water content, carbon chemistry, including CH 4 CO 2 concentrations, but not temperature during first years treatments. Peat aquatic-like signatures (shared predicted protein content marine and/or freshwater vOTUs) enriched more waterlogged surface depths. Predicted host ranges relatively narrow, generally within single bacterial genus. Of 4326 vOTUs, 164 previously detected soils, mostly None identified 202,371 peat, 0.4% 80,714 clusters (VCs, grouped by content) shared between aquatic environments. On per-sample basis, vOTU recovery 32 times viromes total metagenomes. Conclusions suggest strong “species” boundaries terrestrial ecosystems some extent differences less pronounced taxonomic levels. The significant enrichment suggests that may also exhibit niche partitioning on These patterns presumably driven part ecology, consistent narrow ranges. Although samples sequencing depth improved metagenomes, substantially after particle highlights utility viromics.

Язык: Английский

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The hydrogen threshold of obligately methyl-reducing methanogens

FEMS Microbiology Letters, Год журнала: 2020, Номер 367(17)

Опубликована: Авг. 13, 2020

Methanogenesis is the final step in anaerobic degradation of organic matter. The most important substrates methanogens are hydrogen plus carbon dioxide and acetate, but also use methanol, methylated amines, aromatic methoxy groups appears to be more widespread than originally thought. Except for members family Methanosarcinaceae, all methylotrophic require external as reductant therefore compete with hydrogenotrophic this common substrate. Since methanogenesis from consumes four molecules per molecule methane, whereas methanol requires only one, methyl-reducing should have an energetic advantage over at low partial pressures. However, experimental data on their threshold scarce suffers relatively high detection limits. Here, we show that Methanosphaera stadtmanae (Methanobacteriales), Methanimicrococcus blatticola (Methanosarcinales), Methanomassiliicoccus luminyensis (Methanomassiliicoccales) consume pressures < 0.1 Pa, which almost one order magnitude lower thresholds M. reported previous study topic. We conclude outcompete activity limited by availability methyl groups.

Язык: Английский

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