Single-cell metabolomics: where are we and where are we going?

Current Opinion in Biotechnology, Год журнала: 2022, Номер 75, С. 102693 - 102693

Опубликована: Фев. 10, 2022

Single-cell metabolomics with mass spectrometry enables a large variety of metabolites to be simultaneously detected from individual cells, without any preselection or labelling, map phenotypes on the single cell level. Although field is relatively young, it steadily progressing an increasing number active research groups, techniques for sampling and ionization, tools data analysis, applications answer important biomedical environmental questions. In addition, community shows great creativity in overcoming challenges associated low sample volumes, wide range metabolite species, datasets. Here, we briefly discuss publications since 2019 aim provide unfamiliar reader insight into expert update current status field.

Язык: Английский

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All‐In‐One Biomimetic Nanoplatform Based on Hollow Polydopamine Nanoparticles for Synergistically Enhanced Radiotherapy of Colon Cancer

Small, Год журнала: 2022, Номер 18(14)

Опубликована: Фев. 12, 2022

Abstract Even though radiotherapy is the most important therapeutic strategy for colon cancer treatment, there an enormous demand to improve radiosensitivity in solid tumor destruction. For this purpose, a biomimetic nanoplatform based on hollow polydopamine nanoparticles (HP) with homologous targeting and pH‐responsive drug release properties designed. In work, HP constructed by using chelation competition‐induced polymerization then modified cell membrane. Hollow integrated Pt (Pt@HP) has catalase‐like activity, which can be used trigger endogenous H 2 O into , relieving hypoxia of microenvironment (TME). With mesoporous shells large cavities, Pt@HP shows efficient apoptin 100–109 (AP) verteporfin (VP) loading form AVPt@HP@M. Under X‐ray irradiation, AVPt@HP@M exerts radiosensitization effect via multiple strategies, including (Pt NPs), enhancing apoptosis (AP), X‐ray‐induced photodynamic therapy (X‐PDT) (VP). Further metabonomics analysis that specific mechanism through influencing purine metabolism. Without appreciable systemic toxicity, highlights new effective provides reference treating malignant tumors.

Язык: Английский

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Untargeted metabolomics analysis of esophageal squamous cell cancer progression

Journal of Translational Medicine, Год журнала: 2022, Номер 20(1)

Опубликована: Март 14, 2022

Abstract 90% of esophageal cancer are squamous cell carcinoma (ESCC) and ESCC has a very poor prognosis high mortality. Nevertheless, the key metabolic pathways associated with progression haven’t been revealed yet. Metabolomics become new platform for biomarker discovery over recent years. We aim to elucidate dominantly pathway in all tumor/node/metastasis (TNM) stages adjacent cancerous tissues. collected 60 postoperative tissues 15 normal tumor, then performed Liquid Chromatography tandem mass spectrometry (LC–MS/MS) analyses. The metabolites data was analyzed differential correlational expression heatmap according stage I vs. con., II, II III, III IV respectively. Metabolic were acquired by Kyoto Encyclopedia Genes Genomes. (KEGG) database. related genes obtained via Gene Set Enrichment Analysis (GSEA). mRNA detected two public datasets: gene series (GSE)23400 Cancer Genome Atlas (TCGA). Receiver operating characteristic curve (ROC) analysis is applied genes. 712 identified total. Glycerophospholipid metabolism significantly distinct progression. 16 77 glycerophospholipid significance between controls. Phosphatidylserine synthase 1 (PTDSS1) Lysophosphatidylcholine Acyltransferase1 (LPCAT1) had good diagnostic value Area under ROC Curve (AUC) > 0.9 using analysis. In this study, we tumorigenesis could be potential therapeutic target

Язык: Английский

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Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

ACS Central Science, Год журнала: 2023, Номер 9(3), С. 440 - 456

Опубликована: Фев. 21, 2023

Soluble epoxide hydrolase (sEH) plays a critical role in inflammation by modulating levels of epoxyeicosatrienoic acids (EETs) and other epoxy fatty (EpFAs). Here, we investigate the possible sEH lipopolysaccharide (LPS)-mediated macrophage activation acute lung injury (ALI). In this study, found that small molecule, wedelolactone (WED), targeted led to inactivation. Through molecular interaction with amino Phe362 Gln384, WED suppressed activity enhance EETs, thus attenuating oxidative stress regulating glycogen synthase kinase 3beta (GSK3β)-mediated nuclear factor-kappa B (NF-κB) factor E2-related 2 (Nrf2) pathways vitro. an LPS-stimulated ALI animal model, pharmacological inhibition or knockout (KO) alleviated pulmonary damage, such as increase alveolar wall thickness collapse. Additionally, genetic KO both attenuated vivo. These findings provided broader prospects for treatment targeting alleviate suggested natural lead candidate development novel synthetic inhibitors.

Язык: Английский

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Metabolic engineering and synthetic biology strategies for producing high-value natural pigments in Microalgae

Biotechnology Advances, Год журнала: 2023, Номер 68, С. 108236 - 108236

Опубликована: Авг. 14, 2023

Язык: Английский

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