Trends in Plant Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Zoologica Scripta, Год журнала: 2024, Номер 53(6), С. 754 - 762
Опубликована: Июнь 21, 2024
Abstract Molecular markers for tracing animal sample origins and compositions are critical applications such as parasite detection, contamination screening, authentication. Among these, microRNAs have emerged promising candidates due to their deep conservation, near‐hierarchical evolution, stability. I here review the suitability of taxonomic also phylogenetic show how careful annotation efforts establishment curated microRNA gene database MirGeneDB tools like MirMachine revitalized research. These advancements enable accurate studies, highlighting microRNAs' potential in resolving long‐standing questions relationships extending ancient DNA environmental RNA analysis. Future research must focus on expanding complements across all Metazoa further improving methodologies.
Язык: Английский
Процитировано
6
Forensic Science International Genetics, Год журнала: 2025, Номер unknown, С. 103282 - 103282
Опубликована: Апрель 1, 2025
Estimating the age of an unknown perpetrator can be a valuable tool in narrowing down group suspects. Research efforts to estimate stain donor have mainly focused on epigenetic modifications, but there is evidence that RNA expression patterns, i.e. composition transcriptome, change with increasing age, which could promising molecular alternative for prediction. In previous study, we identified total 508 mRNA markers related from two blood whole transcriptome sequencing data sets, using differential analysis DESeq2 and marker selection lasso regression. For this selected both approaches were combined into RNA-specific targeted MPS assay Ion Torrent platform evaluated 100 EDTA samples healthy donors (aged between 23 73 years). We compared three different normalization methods obtained investigated performance various regression techniques The model based elastic net dSVA-normalized exhibited most robust performance, achieving MAE 9.29 years correlation 0.57 chronological predicted age. Although use approach instead RNA-Seq offers several advantages forensic setting, observed considerable amount unwanted variation data. conclude it challenging detect distinct signals associated
Язык: Английский
Процитировано
0
Forensic Science International Genetics, Год журнала: 2024, Номер 72, С. 103089 - 103089
Опубликована: Июнь 19, 2024
Язык: Английский
Процитировано
2
Journal of Archaeological Science, Год журнала: 2024, Номер 174, С. 106132 - 106132
Опубликована: Дек. 18, 2024
Язык: Английский
Процитировано
1
Trends in Microbiology, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
In this review, we delineate the unique set of characteristics associated with cryosphere environments (namely, ice and permafrost) which present both challenges opportunities for studying ancient environmental microbiomes (AEMs). a field currently reliant on several assumptions, discuss theoretical empirical feasibility recovering microbial nucleic acids (NAs) from permafrost varying degrees antiquity. We also summarize contamination control best practices highlight considerations latest approaches, including shotgun metagenomics, downstream bioinformatic authentication approaches. review adoption existing software provide an overview more recently published programs, reference to their suitability AEM studies. Finally, outstanding likely future directions research.
Язык: Английский
Процитировано
0
Trends in Plant Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
0