Current Status of Synthetic Mammalian Embryo Models DOI Open Access
Ha‐Neul Kim, Eunhye Kim

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12862 - 12862

Опубликована: Ноя. 29, 2024

Advances in three-dimensional culture technologies have facilitated the development of synthetic embryo models, such as blastoids, through co-culturing diverse stem cell types. These vitro models enable precise investigation developmental processes, including gastrulation, neurulation, and lineage specification, thereby advancing our understanding early embryogenesis. By providing controllable, ethically viable platforms, they help circumvent limitations vivo mammalian studies contribute to developing regenerative medicine strategies. Nonetheless, ethical challenges, particularly regarding human applications, persist. Comparative across various species—such mice, humans, non-human primates, ungulates, like pigs cattle—offer crucial insights into both species-specific conserved mechanisms. In this review, we outline differences embryonic discuss recent advancements models. Specifically, focus on latest research involving cattle, provide a comprehensive overview improvements technology. biology, improve model efficiency, guide new

Язык: Английский

The establishment of the 3D genome structure during zygotic genome activation DOI Open Access
Anastasiia L. Sivkina, Olga V. Iarovaia, Sergey V. Razin

и другие.

Annals of the New York Academy of Sciences, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Abstract During zygotic genome activation (ZGA) and early development, hierarchical levels of chromatin structure undergo remarkable perturbations: changes in the nuclear‐to‐cytoplasmic ratio various components; accessibility; histone exchange; formation 3D structures such as loops, topologically associated domains, compartments. Here, we review peculiarities, variability, emergence structural features during ZGA different organisms. Focusing on newly found called fountains , describe prerequisites for cohesin loading DNA possible mechanisms organization development. Fountains resulting from asymmetric bidirectional extrusion spread cohesin‐loading points a CTCF‐independent manner. We discuss that may not possess specific functions, unlike conventional structures, could be other biological processes where occurs.

Язык: Английский

Процитировано

0

Epigenome dynamics in early mammalian embryogenesis DOI

Adam Burton,

Maria‐Elena Torres‐Padilla

Nature Reviews Genetics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Язык: Английский

Процитировано

0

Genome folding and zygotic genome activation in mammalian preimplantation embryos DOI Creative Commons
Anastasiia Bondarieva, Kikuë Tachibana

Current Opinion in Genetics & Development, Год журнала: 2024, Номер 89, С. 102268 - 102268

Опубликована: Окт. 9, 2024

Язык: Английский

Процитировано

1

Current Status of Synthetic Mammalian Embryo Models DOI Open Access
Ha‐Neul Kim, Eunhye Kim

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12862 - 12862

Опубликована: Ноя. 29, 2024

Advances in three-dimensional culture technologies have facilitated the development of synthetic embryo models, such as blastoids, through co-culturing diverse stem cell types. These vitro models enable precise investigation developmental processes, including gastrulation, neurulation, and lineage specification, thereby advancing our understanding early embryogenesis. By providing controllable, ethically viable platforms, they help circumvent limitations vivo mammalian studies contribute to developing regenerative medicine strategies. Nonetheless, ethical challenges, particularly regarding human applications, persist. Comparative across various species—such mice, humans, non-human primates, ungulates, like pigs cattle—offer crucial insights into both species-specific conserved mechanisms. In this review, we outline differences embryonic discuss recent advancements models. Specifically, focus on latest research involving cattle, provide a comprehensive overview improvements technology. biology, improve model efficiency, guide new

Язык: Английский

Процитировано

0