Bioactive Materials,
Год журнала:
2024,
Номер
42, С. 194 - 206
Опубликована: Сен. 3, 2024
Men
are
particularly
sensitive
to
penis
size,
especially
those
with
a
deformed
or
injured
penis.
This
can
lead
strong
desire
for
enlargement
surgery.
Given
the
ethical
sensitivities
of
penis,
penile
implants
need
be
developed
both
efficacy
and
safety.
In
this
study,
polyvinyl
alcohol
(PVA)
patch
prepared
via
cyclic
freeze‒thaw
cycles
alkaline
treatment.
The
PVA
hydrogels
treated
5
M
NaOH
had
best
mechanical
properties
stability.
A
negative
Poisson's
ratio
structure
is
incorporated
into
design
patch,
which
allows
it
conform
well
deformation
rabbit
models,
enlarged
patches
effectively
enlarge
without
degradation
fibrosis
while
maintaining
long-term
stability
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(12), С. 10384 - 10384
Опубликована: Июнь 20, 2023
Signaling-pathway
analyses
and
the
investigation
of
gene
responses
to
different
stimuli
are
usually
performed
in
2D
monocultures.
However,
within
glomerulus,
cells
grow
3D
involved
direct
paracrine
interactions
with
glomerular
cell
types.
Thus,
results
from
monoculture
experiments
must
be
taken
caution.
We
cultured
endothelial
cells,
podocytes
mesangial
2D/3D
monocultures
co-cultures
analyzed
survival,
self-assembly,
expression,
cell-cell
interaction,
pathways
using
live/dead
assay,
time-lapse
analysis,
bulk-RNA
sequencing,
qPCR,
immunofluorescence
staining.
Without
any
need
for
scaffolds,
self-organized
into
spheroids.
Podocyte-
cell-specific
markers
extracellular
matrix
were
increased
compared
co-cultures.
Housekeeping
genes
chosen
wisely,
as
many
used
normalization
expression
themselves
affected
culture
conditions.
The
transport
podocyte-derived
VEGFA
confirmed
intercellular
crosstalk
co-culture
models.
enhanced
important
function
3D,
2D,
questions
reliability
currently
Hence,
might
more
suitable
study
communication,
disease
modelling
drug
screening
ex
vivo.
The
function
of
the
glomerulus
depends
on
complex
cell-cell/matrix
interactions
and
replication
this
in
vitro
would
aid
biological
understanding
both
health
disease.
Previous
models
do
not
fully
reflect
all
cell
types
present
as
they
overlook
mesangial
cells
within
their
3D
matrix.
Herein,
development
a
microphysiological
system
that
contains
resident
renal
an
anatomically
relevant
manner
is
presented.
A
detailed
transcriptomic
analysis
contributing
biology
each
type,
well
functionally
appropriate
albumin
retention
system,
demonstrated.
important
role
shown
promoting
maturity
other
types.
Additionally,
comparison
incremental
advances
individual
type
brings
to
phenotype
others
demonstrates
glomerular
simple
2D
culture
exhibit
state
more
reflective
dysfunction
observed
human
disease
than
previously
recognized.
This
model
will
expand
capability
investigate
translatable
by
inclusion
compartment.
Advanced Materials Technologies,
Год журнала:
2023,
Номер
8(7)
Опубликована: Фев. 3, 2023
Blood
vessel
chips
are
bioengineered
microdevices,
consisting
of
biomaterials,
human
cells,
and
microstructures,
which
recapitulate
essential
vascular
structure
physiology
allow
a
well-controlled
microenvironment
spatial-temporal
readouts.
afford
promising
opportunities
to
understand
molecular
cellular
mechanisms
underlying
range
diseases.
The
physiological
relevance
is
key
these
blood
that
rely
on
bioinspired
strategies
bioengineering
approaches
translate
into
artificial
units.
Here,
we
discuss
several
critical
aspects
physiology,
including
morphology,
material
composition,
mechanical
properties,
flow
dynamics,
mass
transport,
provide
guidelines
valuable
source
bioinspiration
for
the
rational
design
chips.
We
also
review
state-of-art
exhibit
important
features
reveal
crucial
insights
biological
processes
disease
pathogenesis,
rare
diseases,
with
notable
implications
drug
screening
clinical
trials.
envision
advances
in
biofabrication,
stem
cells
improve
chips,
which,
along
close
collaborations
between
clinicians
bioengineers,
enable
their
widespread
utility.
Cardiac
fibrosis
has
emerged
as
the
primary
cause
of
morbidity,
disability,
and
even
mortality
in
numerous
nations.
In
light
advancements
precision
medicine
strategies,
substantial
attention
been
directed
toward
development
a
practical
precise
drug
screening
platform
customized
for
individual
patients.
this
study,
we
introduce
biomimetic
cardiac
fibrosis-on-a-chip
incorporating
structural
color
hydrogels
(SCHs)
to
enable
optical
high-throughput
screening.
By
cocultivating
proportion
fibroblasts
(CFBs)
with
cardiomyocytes
on
SCH,
fibrotic
microtissue
successfully
replicates
components
biomechanical
properties
associated
fibrosis.
More
importantly,
shift
observed
SCH
can
be
indicative
contraction
relaxation,
making
it
valuable
tool
evaluating
progression.
such
into
microfluidic
gradient
chip,
develop
that
accurately
efficiently
screens
potential
anti-fibrotic
drugs.
These
characteristics
suggest
microphysiological
possesses
capability
establish
preclinical
framework
drugs,
may
contribute
advancement
medicine.
Bioactive Materials,
Год журнала:
2024,
Номер
42, С. 194 - 206
Опубликована: Сен. 3, 2024
Men
are
particularly
sensitive
to
penis
size,
especially
those
with
a
deformed
or
injured
penis.
This
can
lead
strong
desire
for
enlargement
surgery.
Given
the
ethical
sensitivities
of
penis,
penile
implants
need
be
developed
both
efficacy
and
safety.
In
this
study,
polyvinyl
alcohol
(PVA)
patch
prepared
via
cyclic
freeze‒thaw
cycles
alkaline
treatment.
The
PVA
hydrogels
treated
5
M
NaOH
had
best
mechanical
properties
stability.
A
negative
Poisson's
ratio
structure
is
incorporated
into
design
patch,
which
allows
it
conform
well
deformation
rabbit
models,
enlarged
patches
effectively
enlarge
without
degradation
fibrosis
while
maintaining
long-term
stability
