Cells,
Год журнала:
2024,
Номер
13(23), С. 1973 - 1973
Опубликована: Ноя. 28, 2024
If
the
billions
of
oligodendrocytes
(OLs)
populating
central
nervous
system
(CNS)
patients
could
express
their
feelings,
they
would
undoubtedly
tell
gene
therapists
about
frustration
with
other
neural
cell
populations,
neurons,
microglia,
or
astrocytes,
which
have
been
favorite
targets
transfer
experiments.
This
review
questions
why
OLs
left
out
most
therapy
attempts.
The
first
explanation
is
that
pathogenic
role
still
discussed
in
CNS
diseases.
Another
reason
so-called
ubiquitous
CAG,
CBA,
CBh,
CMV
promoters—widely
used
studies—are
unable
poorly
able
to
activate
transcription
episomal
transgene
copies
brought
by
adeno-associated
virus
(AAV)
vectors
OLs.
Accordingly,
expression
has
either
not
found
evaluated
studies
rodents
non-human
primates.
aims
current
are
give
rightful
place
among
cells
future
target
and
encourage
researchers
test
effect
OL
transduction
various
Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(4), С. 3092 - 3107
Опубликована: Апрель 2, 2024
Autism
spectrum
disorder
(ASD)
is
a
neurodevelopmental
that
includes
autism,
Asperger’s
syndrome,
and
pervasive
developmental
disorder.
Individuals
with
ASD
may
exhibit
difficulties
in
social
interactions,
communication
challenges,
repetitive
behaviors,
restricted
interests.
While
genetic
mutations
individuals
can
either
activate
or
inactivate
the
activities
of
gene
product,
impacting
neuronal
morphogenesis
causing
symptoms,
underlying
mechanism
remains
to
be
fully
established.
Herein,
for
first
time,
we
report
genetically
conserved
Rac1
guanine-nucleotide
exchange
factor
(GEF)
Dock5
signalosome
molecules
control
process
elongation
N1E-115
cell
line,
model
line
capable
achieving
morphological
changes.
The
increased
phenotypes
observed
intellectual
disability
(ID)-associated
Semaphorin-5A
(Sema5A)
Arg676-to-Cys
[p.R676C]
were
also
mediated
by
molecules.
Indeed,
knockdown
using
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/CasRx-based
guide(g)RNA
specifically
recovered
mutated
Sema5A-induced
increase
cells.
Knockdown
Elmo2,
an
adaptor
molecule
Dock5,
exhibited
similar
recovery.
Comparable
results
obtained
when
transfecting
interaction
region
Elmo2.
activation
c-Jun
N-terminal
kinase
(JNK),
one
primary
signal
transduction
elongation,
was
ameliorated
their
transfection.
These
suggest
comprises
abnormal
signaling
involved
induced
ASD-
ID-associated
Sema5A.
could
added
list
potential
therapeutic
target
at
molecular
cellular
levels.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 7, 2024
Diffuse
intrinsic
pontine
gliomas
(DIPGs),
a
major
type
of
pediatric
high-grade
located
in
the
pons,
are
leading
cause
death
children
with
brain
cancer.
A
subset
(20-25%)
DIPGs
harbor
lysine
27-to-methionine
(K27M)
mutation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 23, 2024
Neuronal
activity
promotes
the
proliferation
of
healthy
oligodendrocyte
precursor
cells
(OPC)
and
their
malignant
counterparts,
gliomas.
Many
gliomas
arise
from
closely
resemble
oligodendroglial
lineage
precursors,
including
diffuse
midline
glioma
(DMG),
a
cancer
affecting
structures
such
as
thalamus,
brainstem
spinal
cord.
In
DMG,
glutamatergic
GABAergic
neuronal
progression
through
both
paracrine
signaling
bona-fide
neuron-to-glioma
synapses.
However,
putative
roles
other
subpopulations
-
especially
neuromodulatory
neurons
located
in
that
project
to
long-range
target
sites
anatomical
locations
where
DMGs
remain
largely
unexplored.
Here,
we
demonstrate
cholinergic
midbrain
modulates
OPC
DMG
circuit-specific
manner
at
projections.
Optogenetic
stimulation
pedunculopontine
nucleus
(PPN)
growth
pons,
while
laterodorsal
tegmentum
(LDT)
facilitates
consistent
with
predominant
projection
patterns
each
nucleus.
Reciprocal
was
evident,
increased
PPN
LDT
observed
pontine
DMG-bearing
mice.
co-culture,
hiPSC-derived
form
networks
robustly
promote
proliferation.
Single-cell
RNA
sequencing
analyses
revealed
prominent
expression
muscarinic
receptor
genes
Cells,
Год журнала:
2024,
Номер
13(23), С. 1973 - 1973
Опубликована: Ноя. 28, 2024
If
the
billions
of
oligodendrocytes
(OLs)
populating
central
nervous
system
(CNS)
patients
could
express
their
feelings,
they
would
undoubtedly
tell
gene
therapists
about
frustration
with
other
neural
cell
populations,
neurons,
microglia,
or
astrocytes,
which
have
been
favorite
targets
transfer
experiments.
This
review
questions
why
OLs
left
out
most
therapy
attempts.
The
first
explanation
is
that
pathogenic
role
still
discussed
in
CNS
diseases.
Another
reason
so-called
ubiquitous
CAG,
CBA,
CBh,
CMV
promoters—widely
used
studies—are
unable
poorly
able
to
activate
transcription
episomal
transgene
copies
brought
by
adeno-associated
virus
(AAV)
vectors
OLs.
Accordingly,
expression
has
either
not
found
evaluated
studies
rodents
non-human
primates.
aims
current
are
give
rightful
place
among
cells
future
target
and
encourage
researchers
test
effect
OL
transduction
various
