Systematic Evaluation of Extracellular Coating Matrix on the Differentiation of Human-Induced Pluripotent Stem Cells to Cortical Neurons DOI Open Access
Siyao Li, Yan Liu, Xianyang Luo

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 230 - 230

Опубликована: Дек. 30, 2024

Induced pluripotent stem cell (iPSC)-derived neurons (iNs) have been widely used as models of neurodevelopment and neurodegenerative diseases. Coating culture vessels with extracellular matrixes (ECMs) gives structural support facilitates communication differentiation, ultimately enhances neuronal functions. However, the relevance different ECMs to natural environment their impact on differentiation not fully characterized. In this study, we report use four commonly matrixes, poly-D-lysine (PDL), poly-L-ornithine (PLO), Laminin Matrigel, which applied compare single-coating double-coating conditions iNs maturation. Using IncuCyte live-cell imaging system, found that cultured single Matrigel- Laminin-coated significantly higher density neurite outgrowth branch points than PLO or PDL but produce abnormal highly straight larger body clumps. All reduced clumping neurons, in combination PDL+Matrigel also enhanced purity. Double coating tended improve dendritic axonal development distribution pre postsynaptic markers. These results demonstrate matrix contributes double best outcomes. Our study indicates maturation can be manipulated, a certain extent, by adjusting ECM recipe, provides important technical guidance for neurological studies.

Язык: Английский

Intracellular tension relaxation engineered through D-enantiomeric hydrogel maneuvers neurogenesis and immunomodulation to facilitate spinal cord repair DOI Open Access
Y Y Li, Yulin Wang, Xinyu Fang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

Abstract Microenvironment mechanics plays a vital role in regulating morphogenesis and immune response after injury, but their exploration has been hindered by the fragile mechanical strength oxidative physiological environment spinal cord injury (SCI). Here, we engineered self-assembly hydrogels of enantiomeric peptides with neural tissue-matching properties to persistently manipulate cellular membrane tension mechanotransduction through stereo conformational recognition consequent protein affinity difference. The D-enantiomeric hydrogel-induced intracellular relaxation activates neurogenesis ECM remolding astrocytes, suppresses pro-inflammation promotes pro- regeneration microglia, which significantly facilitates neuroprotection functional recovery rat severe SCI model. relaxation-induced may be characteristics, contrary non-neural cells, as downstream signaling is activated resulted neurogenic morphology change. Overall, inducing potential effective strategy for promoting nerve regeneration.

Язык: Английский

Процитировано

0
Ferroptosis in Neurons: A Bibliometric Analysis of Research Trends, Key Contributions, and Emerging Directions DOI Creative Commons
Shanshan Sun,

Q Q Wang,

Ziyi Zhang

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

Abstract Background Neurons are the fundamental structural and functional units of nervous system, serving as core cells for information transmission regulation. They closely associated with various neurological diseases. Recent studies have shown significant advancements in research on ferroptosis neurons; however, there has been a lack bibliometric analysis this field. This study aims to provide comprehensive overview knowledge structure related neurons through methods, identify current trends hotspots, predict potential future directions. Methods We conducted search publications from 2014 2024 Web Science Core Collection (WoSCC) database. Bibliometric methods were employed analyze authors, institutions, countries, journals, references using VOSviewer, CiteSpace, R package "bibliometrix". Results included 685 articles 50 China United States being leading contributors. The number year-on-year increase. primary institutions Central South University, Harbin Medical University Melbourne. Free Radical Biology Medicine is most popular journal field, while Cell highest citation count. A total 4,673 authors contributed research, David Devos Ashley I. Bush having publications, Scott J. Dixon had co-citations. Keyword revealed that molecular mechanisms its application diseases focuses Conclusion first summarizing developments neurons. outlines predicts global hotspots trends, providing valuable scholars studying ferroptosis.

Язык: Английский

Процитировано

0
Enhanced BBB penetration and microglia-targeting nanomodulator for the two-pronged modulation of chronically activated microglia-mediated neuroinflammation in Alzheimer’s disease DOI Creative Commons

Ya Wei,

Xue Xia, Xiaorong Wang

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Intervention in chronically activated microglia-mediated neuroinflammation is a novel approach to treat Alzheimer's disease (AD). The low permeability of the blood‒brain barrier (BBB) and non-selective distribution brain severely restrict AD drugs' disease-modifying efficacy. Here, an immunosuppressant TREM2-lowing antisense oligonucleotides (ASOs) resveratrol co-loaded cationic liposome developed as immune reprogramming nanomodulator modified by acid-cleavable BBB-targeting peptide microglia-targeting (Res@TcMNP/ASO) for management. Res@TcMNP/ASO can enter endothelial cells via D-T7 peptides. Then undergoes acid-responsive cleavage, facilitating escape Res@MNP/ASO from endo/lysosomes cross BBB. detached specifically targets M1-phenotype microglia exposed MG1 peptides prompt simultaneous delivery two drugs into microglia. This not only restore function through ASO but also mitigate stimulation caused reactive oxygen species (ROS) resveratrol, thereby synergistically inhibiting chronic activation alleviate AD. Our results indicate that this combination treatment achieve significant behavioral cognitive improvements late APP/PS1 mice.

Язык: Английский

Процитировано

0
Spinal Cord Injury Repair Based on Drug and Cell Delivery: from Remodeling Microenvironment to Relay Connection Formation DOI Creative Commons

Wanrong Ma,

Xing Li

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101556 - 101556

Опубликована: Фев. 4, 2025

Spinal cord injury (SCI) presents a formidable challenge in clinical settings, resulting sensory and motor function loss imposing significant personal societal burdens. However, owning to the adverse microenvironment limited regenerative capacity, achieving complete functional recovery after SCI remains elusive. Additionally, traditional interventions including surgery medication have series of limitations that restrict effectiveness treatment. Recently, tissue engineering (TE) has emerged as promising approach for promoting neural regeneration SCI, which can effectively delivery drugs into site cells improve survival differential. Here, we outline main pathophysiology events post injury, further discuss materials common assembly strategies used scaffolds treatment, expound on latest advancements treatment methods based drug cell detail, propose future directions repair with TE highlight potential applications.

Язык: Английский

Процитировано

0
Counteracting immunodepression by extracellular matrix hydrogel to promote brain tissue remodeling and neurological function recovery after traumatic brain injury DOI Creative Commons
Bixue Wang,

qiaoping zhang,

Changsheng Liu

и другие.

Biomaterials, Год журнала: 2025, Номер 318, С. 123181 - 123181

Опубликована: Фев. 8, 2025

Язык: Английский

Процитировано

0
A neurodevelopment-inspired self-evolving scaffold for nerve regeneration DOI Creative Commons

Yizhu Shan,

Lingling Xu, Xi Cui

и другие.

Опубликована: Фев. 1, 2025

Процитировано

0
A subtype of T cells impedes tissue repair in aged spinal cord after injury DOI
Chengle Zhang, Kai Liu

Neuron, Год журнала: 2025, Номер 113(5), С. 643 - 645

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Centrosomal and acentrosomal microtubule nucleation during neuronal development DOI Creative Commons
Stanislav Vinopal, Frank Bradke

Current Opinion in Neurobiology, Год журнала: 2025, Номер 92, С. 103016 - 103016

Опубликована: Март 28, 2025

Язык: Английский

Процитировано

0
Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat DOI Creative Commons
Wei Zhao,

Kun-Xiu Song,

Bing-Dong Ma

и другие.

BMC Surgery, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 11, 2025

Peripheral nerve injury (PNI) is a common condition that leads to the partial loss of function in sensory, motor, and autonomic nervous systems. The peripheral system has an inherent capacity regenerate after re-innervate its target organs, but full functional recovery rare. In recent years, there been growing interest identifying drugs can promote axonal regeneration outgrowth following PNI. Epothilone B (EpoB) FDA-approved antineoplastic agent promotes tubulin polymerization enhances stability microtubules. Recently, regenerative effects EpoB central have garnered attention, potential therapeutic on remain underexplored. This study utilized sciatic transection anastomosis model rats evaluate neuroprotein expression injury. Behavioral analysis, Masson's trichrome staining, immunofluorescence staining were conducted assess impact regeneration. Over time, motor muscle reinnervation observed, with Sciatic Functional Index (SFI) scores higher EpoB-treated group compared vehicle group. fibronectin (FN) was significantly lower group, while Tau, neurofilament-M (NF-M), growth-associated protein-43 (GAP-43) higher. conclusion, treatment increases NF-M, GAP-43, suggesting positive effect repair.

Язык: Английский

Процитировано

0
Nervous System Repair and Immunity: Chiral Effects of Biomimetic Self‐Assembled Neuropeptide Hydrogels DOI
Xiaolin Zhou,

Yanqiu Guo,

Yi Wei

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract Function‐encoding peptides have emerged as promising biomaterials capable of replicating the robust biological functions extracellular matrix (ECM). Nevertheless, full potential their sequence designability remains to be explored develop highly bioactive peptide‐based with minimal immunogenicity. In this study, chiral are self‐assembled into supramolecular hydrogels (FFFKTTKS/fffkttks) incorporating an active derived from collagen hydrolysis, a key ECM factor. While FFFKTTKS (L‐type) and fffkttks (D‐type) exhibit comparable viscoelasticity, porosity, architecture, they differ in nanofiber composition, particularly helical orientation. model spinal cord injury, hydrogel demonstrates superior neuronal regeneration motor function recovery compared its counterpart. Further investigations reveal that both equally promote expression ECM‐related genes, subsequently regulating nerve cell adhesion, differentiation, synaptic regeneration. Notably, elicits mild immune response exhibits moderate anti‐inflammatory properties. contrast, triggers response, activating TNF pathway microglia vivo. These findings underscore nanoscale superstructures specific peptide sequences can effectively modulate biocapability neuroregeneration, providing critical insights for rational design synthetic ECM.

Язык: Английский

Процитировано

0