Multi‐Omics Analysis Reveals Disturbances of Purine Metabolism and Glutamate Metabolism in the Hippocampus of Lipopolysaccharide‐Induced Mouse Model of Depression
Brain and Behavior,
Год журнала:
2025,
Номер
15(5)
Опубликована: Май 1, 2025
ABSTRACT
Background
Depression
is
a
global
health
concern
characterized
by
high
incidence,
disability,
and
disease
burden.
Neuroimmunity,
through
the
secretion
of
inflammatory
mediators
mediation
neuroinflammation,
plays
significant
role
in
depression's
pathogenesis.
However,
underlying
molecular
mechanisms
remain
poorly
understood.
Methods
In
this
pioneering
study,
we
employed
comprehensive
multi‐omics
approach,
integrating
2‐DE
proteomics,
liquid
chromatography
mass
spectrometry‐based
metabolomics,
real‐time
polymerase
chain
reaction
(PCR)
array,
to
investigate
hippocampal
profiles
lipopolysaccharide
(LPS)‐induced
immune
inflammation‐related
depression.
This
innovative
approach
aimed
explore
potential
pathogenesis
depression
systematically
data
across
multiple
layers.
Results
Compared
control
group,
identified
81
differential
proteins,
44
metabolites,
4
mRNAs
LPS‐treated
mice.
Integrated
analysis
these
multidimensional
revealed
that
purine
metabolism
glutamate
are
most
significantly
altered
pathways
LPS‐induced
Additionally,
constructed
corresponding
compound‐reaction‐enzyme‐gene
regulatory
network.
Conclusion
study
suggests
may
be
which
neuroinflammation
regulates
depression‐like
behaviors.
Our
findings
confirm
important
inflammation
provide
new
clue
for
diagnosis
treatment
disorder.
Notably,
represents
effort
field,
providing
unprecedented
insights
into
Язык: Английский
Nicotinamide mononucleotide supplementation in aging male animals affects lipoxygenase related arachidonic acid metabolism pathway by regulating intracellular calcium overload
Journal of Functional Foods,
Год журнала:
2025,
Номер
129, С. 106854 - 106854
Опубликована: Май 12, 2025
Язык: Английский
Lipidomic Profiling Reveals HSD17B13 Deficiency-Associated Dysregulated Hepatic Phospholipid Metabolism in Aged Mice
Metabolites,
Год журнала:
2025,
Номер
15(6), С. 353 - 353
Опубликована: Май 27, 2025
Objectives:
HSD17B13
(17β-hydroxysteroid
dehydrogenase
13),
a
lipid
droplet-associated
enzyme,
has
emerged
as
key
regulator
of
hepatic
metabolism
and
potential
therapeutic
target
for
metabolic-associated
fatty
liver
disease
(MAFLD).
While
its
role
in
homeostasis
inflammation
been
partially
revealed,
the
impact
deficiency
on
aged
mice
remains
poorly
understood.
In
this
study,
we
performed
comprehensive
lipidomic
profiling
tissues
from
Hsd17b13
gene
knockout
(Hsd17b13
KO)
to
investigate
effects
deletion
composition
metabolic
pathways.
Methods:
Changes
profiles
were
assessed
through
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)-based
analysis.
Results:
The
profiles,
including
triglycerides
(TGs),
diglycerides
(DGs),
phosphatidylcholines
(PCs),
phosphatidylethanolamines
(PEs),
phosphatidylglycerols
(PGs),
ceramides
(Cers),
exhibited
notable
alterations
KO
mice.
Conclusions:
plays
pivotal
during
aging,
it
is
involved
regulation
phospholipid
metabolism.
Our
study
highlights
importance
maintaining
age-related
diseases.
Язык: Английский