Cnidarian Pattern Recognition Receptor Repertoires Reflect Both Phylogeny and Life History Traits DOI Creative Commons
Madison A. Emery, Bradford Dimos, Laura D. Mydlarz

и другие.

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Июнь 23, 2021

Pattern recognition receptors (PRRs) are evolutionarily ancient and crucial components of innate immunity, recognizing danger-associated molecular patterns (DAMPs) activating host defenses. Basal non-bilaterian animals such as cnidarians must rely solely on immunity to defend themselves from pathogens. By investigating cnidarian PRR repertoires we can gain insight into the evolution in these basal animals. Here utilize increasing amount available genomic resources within Cnidaria survey downstream immune pathway completeness 15 species spanning two major clades, Anthozoa Medusozoa. Overall, find that anthozoans possess prototypical PRRs, while medusozoans appear lack proteins. Additionally, consistently had higher numbers PRRs across all four classes relative medusozoans, a trend largely driven by expansions NOD-like C-type lectins. Symbiotic, sessile, colonial also have expanded their non-symbiotic, mobile, solitary counterparts. Interestingly, seem key mammalian pathways, though similar numbers, more complete pathways than medusozoans. Together, our data indicate greater specificity which hypothesize be due life history traits common Anthozoa. this investigation reveals important insights proteins

Язык: Английский

Mapping single-cell atlases throughout Metazoa unravels cell type evolution DOI Creative Commons
Alexander J. Tarashansky, Jacob M. Musser, Margarita Khariton

и другие.

eLife, Год журнала: 2021, Номер 10

Опубликована: Май 4, 2021

Comparing single-cell transcriptomic atlases from diverse organisms can elucidate the origins of cellular diversity and assist annotation new cell atlases. Yet, comparison between distant relatives is hindered by complex gene histories diversifications in expression programs. Previously, we introduced self-assembling manifold (SAM) algorithm to robustly reconstruct manifolds data (Tarashansky et al., 2019). Here, build on SAM map atlas across species. This method, SAMap, identifies homologous types with shared programs species within phyla, even examples where tissues emerge distinct germ layers. SAMap also finds many genes more similar their paralogs than orthologs, suggesting paralog substitution may be common evolution previously appreciated. Lastly, comparing animal spanning sponge mouse, reveals ancient contractile stem families, which have arisen early evolution.

Язык: Английский

Процитировано

215
A stony coral cell atlas illuminates the molecular and cellular basis of coral symbiosis, calcification, and immunity DOI Creative Commons
Shani Levy, Anamaria Elek, Xavier Grau‐Bové

и другие.

Cell, Год журнала: 2021, Номер 184(11), С. 2973 - 2987.e18

Опубликована: Май 1, 2021

Stony corals are colonial cnidarians that sustain the most biodiverse marine ecosystems on Earth: coral reefs. Despite their ecological importance, little is known about cell types and molecular pathways underpin biology of reef-building corals. Using single-cell RNA sequencing, we define over 40 across life cycle Stylophora pistillata. We discover specialized immune cells, uncover developmental gene expression dynamics calcium-carbonate skeleton formation. By simultaneously measuring transcriptomes cells algae within them, characterize metabolic programs involved in symbiosis both partners. also trace evolution these specializations by phylogenetic integration multiple cnidarian type atlases. Overall, this study reveals cellular basis stony biology.

Язык: Английский

Процитировано

173
Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development DOI Creative Commons
Abhinav Sur, Yiqun Wang,

Paulina Capar

и другие.

Developmental Cell, Год журнала: 2023, Номер 58(24), С. 3028 - 3047.e12

Опубликована: Ноя. 22, 2023

Язык: Английский

Процитировано

89
Ancient plant-like terpene biosynthesis in corals DOI
Immo Burkhardt, Tristan de Rond, Percival Yang-Ting Chen

и другие.

Nature Chemical Biology, Год журнала: 2022, Номер 18(6), С. 664 - 669

Опубликована: Май 23, 2022

Язык: Английский

Процитировано

78
The coral microbiome: towards an understanding of the molecular mechanisms of coral–microbiota interactions DOI Creative Commons
Amin R. Mohamed,

Michael A. Ochsenkühn,

Ahmed M Kazlak

и другие.

FEMS Microbiology Reviews, Год журнала: 2023, Номер 47(2)

Опубликована: Март 1, 2023

Corals live in a complex, multipartite symbiosis with diverse microbes across kingdoms, some of which are implicated vital functions, such as those related to resilience against climate change. However, knowledge gaps and technical challenges limit our understanding the nature functional significance complex symbiotic relationships within corals. Here, we provide an overview complexity coral microbiome focusing on taxonomic diversity functions well-studied cryptic microbes. Mining literature indicate that while corals collectively harbour third all marine bacterial phyla, known symbionts antagonists represent minute fraction this these taxa cluster into select genera, suggesting selective evolutionary mechanisms enabled bacteria gain niche holobiont. Recent advances research aimed at leveraging manipulation increase coral's fitness help mitigate heat stress-related mortality discussed. Then, insights potential through microbiota can communicate modify host responses examined by describing recognition patterns, microbially derived epigenome effector proteins gene regulation. Finally, power omics tools used study highlighted emphasis integrated host-microbiota multiomics framework understand underlying during change-driven dysbiosis.

Язык: Английский

Процитировано

58
The Molecular Language of the Cnidarian–Dinoflagellate Symbiosis DOI
Sabrina L. Rosset, Clinton A. Oakley, Christine Ferrier‐Pagès

и другие.

Trends in Microbiology, Год журнала: 2020, Номер 29(4), С. 320 - 333

Опубликована: Окт. 8, 2020

Язык: Английский

Процитировано

111
Insights into coral bleaching under heat stress from analysis of gene expression in a sea anemone model system DOI
Phillip A. Cleves, Cory J. Krediet, Erik Lehnert

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2020, Номер 117(46), С. 28906 - 28917

Опубликована: Ноя. 9, 2020

Significance Coral reefs are biodiversity hotspots of great ecological, economic, and aesthetic importance. Their global decline under climate change other stresses makes it urgent to understand the molecular bases their responses stress, including “bleaching,” in which corals' photosynthetic algal symbionts lost, thus depriving host animals a crucial source energy metabolic building blocks. We sought clues mechanisms that cause (or protect against) bleaching by analyzing patterns gene expression sea anemone relative corals during exposure heat stress sufficient induce bleaching. The results challenge some current ideas about while also suggesting hypotheses identifying genes prime targets for future genetic analyses.

Язык: Английский

Процитировано

75
Evolutionary cell type mapping with single-cell genomics DOI
Amos Tanay, Arnau Sebé-Pedrós

Trends in Genetics, Год журнала: 2021, Номер 37(10), С. 919 - 932

Опубликована: Май 18, 2021

Язык: Английский

Процитировано

65
Topological structures and syntenic conservation in sea anemone genomes DOI Creative Commons
Bob Zimmermann, Juan D. Montenegro, Sofia Robb

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Дек. 13, 2023

Abstract There is currently little information about the evolution of gene clusters, genome architectures and karyotypes in early branching animals. Slowly evolving anthozoan cnidarians can be particularly informative these features. Here we report chromosome-level assemblies two related anthozoans, sea anemones Nematostella vectensis Scolanthus callimorphus . We find a robust set 15 chromosomes with clear one-to-one correspondence between species. Both genomes show chromosomal conservation, allowing us to reconstruct ancestral cnidarian metazoan blocks, consisting at least 19 16 linkage groups, respectively. that, contrast Bilateria, Hox NK clusters investigated are largely disintegrated, despite presence staggered hox/gbx expression This loss microsynteny conservation may facilitated by shorter distances cis-regulatory sequences their cognate transcriptional start sites. no evidence for topologically associated domains, suggesting fundamental differences long-range regulation compared vertebrates. These data suggest that large sets genes have been retained groups some extant lineages; yet, higher order 3D architecture evolved only after cnidarian-bilaterian split.

Язык: Английский

Процитировано

34
Molecular insights into the Darwin paradox of coral reefs from the sea anemone Aiptasia DOI Creative Commons
Guoxin Cui, Migle K. Konciute, Lorraine Ling

и другие.

Science Advances, Год журнала: 2023, Номер 9(11)

Опубликована: Март 15, 2023

Symbiotic cnidarians such as corals and anemones form highly productive biodiverse coral reef ecosystems in nutrient-poor ocean environments, a phenomenon known Darwin's paradox. Resolving this paradox requires elucidating the molecular bases of efficient nutrient distribution recycling cnidarian-dinoflagellate symbiosis. Using sea anemone Aiptasia, we show that during symbiosis, increased availability glucose presence algae jointly induce coordinated up-regulation relocalization ammonium transporters. These responses are critical to support symbiont functioning organism-wide nitrogen assimilation through glutamine synthetase/glutamate synthase-mediated amino acid biosynthesis. Our results reveal crucial aspects mechanisms underlying conservation these organisms allow them thrive nitrogen-poor environments.

Язык: Английский

Процитировано

28