Molecular Biology of the Cell,
Год журнала:
2023,
Номер
34(6)
Опубликована: Янв. 25, 2023
Cells
sense
and
migrate
across
mechanically
dissimilar
environments
throughout
development
disease
progression.
However,
it
remains
unclear
whether
mechanical
memory
of
past
empowers
cells
to
navigate
new,
three-dimensional
extracellular
matrices.
Here,
we
show
that
previously
primed
on
stiff,
compared
with
soft,
matrices
generate
a
higher
level
forces
remodel
collagen
fibers
promote
invasion.
This
priming
advantage
persists
in
dense
or
stiffened
collagen.
We
explain
this
memory-dependent,
cross-environment
cell
invasion
through
lattice-based
model
wherein
stiff-primed
cellular
minimize
energy
required
for
future
According
our
model,
transfer
their
the
matrix
via
alignment
tension,
remodeled
informs
Thus,
memory-laden
overcome
mechanosensing
softer
challenging
cell-matrix
memory.
Consistent
predictions,
depletion
yes-associated
protein
destabilizes
remodeling
before
release
tension
laser
ablation
disable
fiber
by
lysyl-oxidase
inhibition,
both
which
disrupt
cell-to-matrix
hamper
These
results
have
implications
cancer,
fibrosis,
aging,
where
potential
may
prolonged
response.
Advanced Materials,
Год журнала:
2023,
Номер
35(16)
Опубликована: Март 9, 2023
Abstract
The
extracellular
matrix
is
the
biophysical
environment
that
scaffolds
mammalian
cells
in
body.
main
constituent
collagen.
In
physiological
tissues,
collagen
network
topology
diverse
with
complex
mesoscopic
features.
While
studies
have
explored
roles
of
density
and
stiffness,
impact
architectures
remains
not
well‐understood.
Developing
vitro
systems
recapitulate
these
critical
for
understanding
physiologically
relevant
cell
behaviors.
Here,
methods
are
developed
to
induce
formation
heterogeneous
architectures,
referred
as
islands,
hydrogels.
These
island‐containing
gels
highly
tunable
inclusions
mechanical
properties.
Although
globally
soft,
there
regional
enrichment
concentration
at
cell‐scale.
Collagen‐island
utilized
study
mesenchymal
stem
behavior,
it
demonstrated
migration
osteogenic
differentiation
altered.
Finally,
induced
pluripotent
cultured
gels,
shown
architecture
sufficient
mesodermal
differentiation.
Overall,
this
work
highlights
tissue
bioactive
cues
regulating
behavior
presents
a
novel
collagen‐based
hydrogel
captures
features
engineering
applications.
Annual Review of Biomedical Engineering,
Год журнала:
2024,
Номер
26(1), С. 93 - 118
Опубликована: Фев. 5, 2024
Cell
traction
force
plays
a
critical
role
in
directing
cellular
functions,
such
as
proliferation,
migration,
and
differentiation.
Current
understanding
of
cell
is
largely
derived
from
2D
measurements
where
cells
are
plated
on
substrates.
However,
do
not
recapitulate
vital
aspect
living
systems;
that
is,
actively
remodel
their
surrounding
extracellular
matrix
(ECM),
the
remodeled
ECM,
return,
can
have
profound
impact
phenotype
generation.
This
reciprocal
adaptivity
systems
encoded
material
properties
biological
gels.
In
this
review,
we
summarize
recent
progress
measuring
for
embedded
within
3D
gels,
with
an
emphasis
cell-ECM
cross
talk.
We
also
provide
perspectives
tools
techniques
could
be
adapted
to
measure
complex
biochemical
biophysical
environments.
Journal of Cell Science,
Год журнала:
2024,
Номер
137(8)
Опубликована: Апрель 15, 2024
Cell
migration
is
controlled
by
the
coordinated
action
of
cell
adhesion,
cytoskeletal
dynamics,
contractility
and
extrinsic
cues.
Integrins
are
main
adhesion
receptors
to
ligands
extracellular
matrix
(ECM),
linking
actin
cytoskeleton
ECM
enabling
cells
sense
rigidity
mount
a
directional
response
stiffness
gradients.
Most
models
studied
show
preferred
single
or
clusters
towards
increasing
rigidity.
This
referred
as
durotaxis,
since
its
initial
discovery
in
2000,
technical
advances
elegant
computational
have
provided
molecular
level
details
sensing
migration.
However,
modeling
has
long
predicted
that,
depending
on
intrinsic
factors,
such
balance
molecules
(clutches)
motor
proteins
pulling
them,
might
also
prefer
intermediate
Recently,
experimental
evidence
supported
this
notion
demonstrated
ability
migrate
lower
rigidity,
process
called
negative
durotaxis.
In
Review,
we
discuss
significant
conceptual
that
been
made
our
appreciation
plasticity
context
dependency
stiffness-guided
Molecular Biology of the Cell,
Год журнала:
2023,
Номер
34(6)
Опубликована: Янв. 25, 2023
Cells
sense
and
migrate
across
mechanically
dissimilar
environments
throughout
development
disease
progression.
However,
it
remains
unclear
whether
mechanical
memory
of
past
empowers
cells
to
navigate
new,
three-dimensional
extracellular
matrices.
Here,
we
show
that
previously
primed
on
stiff,
compared
with
soft,
matrices
generate
a
higher
level
forces
remodel
collagen
fibers
promote
invasion.
This
priming
advantage
persists
in
dense
or
stiffened
collagen.
We
explain
this
memory-dependent,
cross-environment
cell
invasion
through
lattice-based
model
wherein
stiff-primed
cellular
minimize
energy
required
for
future
According
our
model,
transfer
their
the
matrix
via
alignment
tension,
remodeled
informs
Thus,
memory-laden
overcome
mechanosensing
softer
challenging
cell-matrix
memory.
Consistent
predictions,
depletion
yes-associated
protein
destabilizes
remodeling
before
release
tension
laser
ablation
disable
fiber
by
lysyl-oxidase
inhibition,
both
which
disrupt
cell-to-matrix
hamper
These
results
have
implications
cancer,
fibrosis,
aging,
where
potential
may
prolonged
response.
