Heterogeneous model for superdiffusive movement of dense core vesicles in C. elegans DOI Creative Commons
Anna Gavrilova, Nickolay Korabel, Viki Allan

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 27, 2025

Abstract Transport of dense core vesicles (DCVs) in neurons is crucial for distributing molecules like neuropeptides and growth factors. We studied the experimental trajectories dynein-driven directed movement DCVs ALA neuron C. elegans over a duration up to 6 seconds. analysed DCV three strains : (1) with normal kinesin-1 function, (2) reduced function kinesin light chain 2 (KLC-2), (3) null mutation 1 (KLC-1). find that move superdiffusively displacement variance $$var(x) \sim t^2$$ all low reversal rates frequent immobilization DCVs. The distribution displacements fits beta-binomial mean following linear quadratic patterns, respectively. propose simple heterogeneous random walk model explain observed superdiffusive retrograde transport behaviour movement. This involves probability beta density resume its or remain same position. To validate our further, we measure first passage time reach certain threshold time. According model, should follow beta-negative binomial parameters as distributions. Our data confirm this prediction.

Язык: Английский

A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 210 - 219

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

22
Brainstem neuropeptidergic neurons link a neurohumoral axis to satiation DOI
Srikanta Chowdhury,

Nachiket G. Kamatkar,

Wendy Xueyi Wang

и другие.

Cell, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

4
Hierarchical regulation of functionally antagonistic neuropeptides expressed in a single neuron pair DOI Creative Commons
Ichiro Aoki, Luca Golinelli,

Eva Dunkel

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 3, 2024

Abstract Neuronal communication involves small-molecule transmitters, gap junctions, and neuropeptides. While neurons often express multiple neuropeptides, our understanding of the coordination their actions mutual interactions remains limited. Here, we demonstrate that two NLP-10 FLP-1, released from same interneuron pair, AVKL/R, exert antagonistic effects on locomotion speed in Caenorhabditis elegans . accelerates by activating G protein-coupled receptor NPR-35 premotor interneurons promote forward movement. Notably, establish is crucial for aversive response to mechanical noxious light stimuli. Conversely, AVK-derived FLP-1 slows down suppressing secretion AVK, through autocrine feedback via activation its DMSR-7 AVK neurons. Our findings suggest peptidergic motifs, exemplified interaction between might represent a widespread mechanism nervous systems across species. These functional among co-transmitters could fine-tune brain activity.

Язык: Английский

Процитировано

12
The fly connectome reveals a path to the effectome DOI Creative Commons
Dean A. Pospisil, Max Jameson Aragon, Sven Dorkenwald

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 201 - 209

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

10
The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses DOI Creative Commons
Ya Wang,

Chun Hin Chow,

Yu Zhang

и другие.

PLoS Biology, Год журнала: 2025, Номер 23(2), С. e3003023 - e3003023

Опубликована: Фев. 6, 2025

The balance between synaptic excitation and inhibition (E/I) is essential for coordinating motor behavior, yet the differential roles of exocytosis regulators in this are less understood. In study, we investigated 2 conserved regulators, complexin/CPX-1 CAPS/UNC-31, excitatory versus inhibitory synapses at Caenorhabditis elegans neuromuscular junctions. cpx-1 null mutants exhibited a marked increase spontaneous release specifically synapses, alongside an unequal reduction evoked release. A clamping-specific knockin mutant, cpx-1(Δ12) , which preserved release, also showed biased enhancement Conversely, unc-31 mutation, while maintaining normal displayed more pronounced synapses. Notably, found that CPX-1’s clamping function dependent on UNC-31 sensitive to external Ca 2+ . Pull-down experiments confirmed CAPS/UNC-31 does not directly interact with complexin, implying indirect regulatory mechanism. Moreover, complexin regulates activity-dependent plasticity, dependent. unexpected role absence CPX-1 may underpin E/I coordinated behavioral outputs different species.

Язык: Английский

Процитировано

2
Norepinephrine changes behavioral state through astroglial purinergic signaling DOI
Alex Chen, Marc Duque,

Altyn Rymbek

и другие.

Science, Год журнала: 2025, Номер 388(6748), С. 769 - 775

Опубликована: Май 15, 2025

Both neurons and glia communicate through diffusible neuromodulators; however, how neuron-glial interactions in such neuromodulatory networks influence circuit computation behavior is unclear. During futility-induced behavioral transitions the larval zebrafish, neuromodulator norepinephrine (NE) drives fast excitation delayed inhibition of activity. We found that astroglial purinergic signaling implements inhibitory arm this motif. In NE triggers release adenosine triphosphate (ATP), extracellular conversion ATP into adenosine, suppression activation hindbrain neuronal receptors. Our results suggest a computational role for an evolutionarily conserved axis NE-mediated brain state position astroglia as important effectors signaling.

Язык: Английский

Процитировано

2
Norepinephrine changes behavioral state via astroglial purinergic signaling DOI Creative Commons
Alex Chen, Marc Duque, Vickie M. Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 23, 2024

Both neurons and glia communicate via diffusible neuromodulatory substances, but the substrates of computation in such networks are unclear. During behavioral transitions larval zebrafish, neuromodulator norepinephrine drives fast excitation delayed inhibition behavior circuit activity. We find that inhibitory arm this feedforward motif is implemented by astroglial purinergic signaling. Neuromodulator imaging, pharmacology, perturbations astroglia reveal triggers release adenosine triphosphate, extracellular conversion into adenosine, suppression through activation hindbrain neuronal receptors. This work, along with a companion piece Lefton colleagues demonstrating an analogous pathway mediating effect on synaptic connectivity mice, identifies computational role for evolutionarily conserved signaling axis norepinephrine-mediated brain state transitions.

Язык: Английский

Процитировано

8
Microscale droplet assembly enables biocompatible multifunctional modular iontronics DOI
Yujia Zhang, Cheryl Tan, Christopher N. Toepfer

и другие.

Science, Год журнала: 2024, Номер 386(6725), С. 1024 - 1030

Опубликована: Ноя. 28, 2024

Hydrogel iontronic devices can emulate biological functions and communicate with living matter. But the fabrication of miniature, soft according to modular designs has not been achieved. In this work, we report use surfactant-supported assembly freestanding microscale hydrogel droplets construct various modules, circuits, biointerfaces. Chemical modifications silk fibroin produced a pair oppositely charged hydrogels. Microscale combinations diodes, npn- pnp-type transistors, diverse reconfigurable logic gates. Through incorporation poly(amino acid)s, have demonstrated droplet-based synthetic synapse ionic polymer-mediated long-term plasticity. Further, our transistor serve as biocompatible sensor record electrophysiological signals from sheets human cardiomyocytes, paving way building miniature bioiontronic systems.

Язык: Английский

Процитировано

7
PLGA scaffold combined with MSCs transplantation improved neural function and brain tissue structure in rats with traumatic brain injury DOI Creative Commons
Na Xu,

Zihuan Sun,

Weikang Guan

и другие.

Brain Research Bulletin, Год журнала: 2025, Номер unknown, С. 111216 - 111216

Опубликована: Янв. 1, 2025

Poly (lactic-co-glycolic acid) (PLGA) is an important biomaterial for tissue defect repair, but its application in replacing missing brain needs improvement. Mesenchymal stem cells (MSCs) have been used to treat various neurological diseases, they face challenges when filling large defects. The purpose of this study was investigate the effects PLGA combined with MSCs transplantation on structure and neural function rats traumatic injury (TBI), explore possible mechanism. results showed that both PLGA+MSCs could improve promote nerve recovery TBI, being superior transplantation. Furthermore, compared alone, further reduced cell apoptosis, promoted neuron survival, improved synaptic plasticity. Overall, adhesion can enhance therapeutic efficacy following TBI.

Язык: Английский

Процитировано

1
A new approach for estimating effective connectivity from activity in neural networks DOI Creative Commons

Niklas Laasch,

Wilhelm Braun,

Lisa Knoff

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 6, 2024

Abstract Inferring and understanding the underlying connectivity structure of a system solely from observed activity its constituent components is challenge in many areas science. In neuroscience, techniques for estimating are paramount when attempting to understand network neural systems their recorded patterns. To date, no universally accepted method exists inference effective connectivity, which describes how node mechanistically affects other nodes. Here, focussing on purely excitatory networks small intermediate size continuous dynamics, we provide systematic comparison different approaches connectivity. Starting with Hopf neuron model conjunction known ground truth structural reconstruct system’s matrix using variety algorithms. We show that, sparse non-linear delays, combining lagged-cross-correlation (LCC) approach recently published derivative-based covariance analysis provides most reliable estimation matrix. also that linear networks, LCC has comparable performance based transfer entropy, at drastically lower computational cost. highlight works best decreases larger less networks. Applying dynamics without time find it does not outperform methods. Employing model, then use estimated as basis forward simulation order recreate under certain conditions, method, LCC, results higher trace-to-trace correlations than methods noise-driven systems. Finally, apply empirical biological data. subset nervous nematode C. Elegans . computationally simple performs better another published, more expensive reservoir computing-based method. Our comparatively can be used reliably estimate directed presence spatio-temporal delays noise. concrete suggestions scenario common research, where only neuronal set neurons known.

Язык: Английский

Процитировано

6