Tolerance to multiple abiotic stresses is mediated by interacting CNGC proteins that regulate Ca²⁺ influx and stomatal movement in rice

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

ABSTRACT Members of the cyclic nucleotide‐gated channel (CNGC) proteins are reportedly involved in a variety biotic and abiotic responses stomatal movement. However, it is unknown if how single member could regulate multiple responses. Here we characterized three closely related CNGC genes rice, OsCNGC14 , OsCNGC15 OsCNGC16 to determine whether they function stresses. The loss‐of‐function mutants each these had reduced calcium ion (Ca 2+ ) influx slower closure response heat, chilling, drought stress hormone abscisic acid (ABA). These also tolerance chilling compared with wild‐type. Conversely, overexpression led more rapid stresses enhanced drought. tight association strongly suggests that conferred by OsCNGC results at least partially from their regulation In addition, physical interactions were observed among but not distantly CNGC, suggesting formation hetero‐oligomers themselves. This study unveils crucial role OsCNGC14, 15 16 stresses, mechanism involves movement regulation.

Язык: Английский

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The heat shock response of plants: new insights into modes of perception and signaling and how hormones contribute

Journal of Experimental Botany, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

Abstract Plants have evolved specific temperature preferences, and shifts above this range cause heat stress with detrimental effects such as physiological disruptions, metabolic imbalances, growth arrest. To reduce damage, plants utilize the shock response (HSR), signaling cascades that activate factors (HSFs), transcription control stress-responsive transcriptome for activation of protective measures. While core HSR is well studied, we still know relatively little about perception signal integration or crosstalk other pathways. In last few years, however, significant progress has been made in area, which summarized here. It emerged plant hormones brassinosteroids (BRs) abscisic acid (ABA) contribute to tolerance by impacting modes activity HSFs. Also, began understand sensed different cellular compartments events nucleus, nuclear condensate formation via liquid–liquid phase separation, play a key role. future, it will be important explore how these multilayered are utilized environmental context developmental stage determine outcome on development.

Язык: Английский

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The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre‐mRNA in rice

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Summary Heat stress significantly impacts global rice production, highlighting the critical need to understand genetic basis of heat resistance in rice. U2AF ( U2 snRNP a uxiliary f actor) is an essential splicing complex with roles recognizing 3′‐splice site precursor messenger RNAs (pre‐mRNAs). The small subunit (U2AF35) can bind 3′‐AG intron border and promote binding branch‐point sequences introns through interaction large (U2AF65). However, functions U2AF35 plants are poorly understood. In this study, we discovered that OsU2AF35a gene was vigorously induced by could positively regulate thermotolerance during both seedling reproductive growth stages. interacts OsU2AF65a within nucleus, them form condensates liquid–liquid phase separation (LLPS) following stress. intrinsically disordered regions (IDR) accountable for their LLPS. condensation indispensable thermotolerance. RNA‐seq analysis disclosed that, subsequent treatment, expression levels several genes associated water deficiency oxidative osu2af35a‐1 were markedly lower than those ZH11. accordance this, capable regulating pre‐mRNAs considerable number mutant exhibited defective splicing, among which OsHSA32 gene. Knocking out reduced rice, while overexpressing partially rescue sensitivity . Together, our findings uncovered role response protein alternative pre‐mRNA splicing.

Язык: Английский

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Impact of High-Temperature Stress on Maize Seed Setting: Cellular and Molecular Insights of Thermotolerance

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1283 - 1283

Опубликована: Фев. 2, 2025

Global warming poses a significant threat to crop production and food security, with maize (Zay mays L.) particularly vulnerable high-temperature stress (HTS). This review explores the detrimental impacts of elevated temperatures on development across various growth stages, analyzed within source–sink framework, particular focus seed setting yield reduction. It provides broad analysis cellular molecular responses HTS, highlighting key roles plant hormone abscisic acid (ABA) signaling, calcium chloroplast, DNA damage repair (DDR) system in maize. HTS disrupts ABA signaling pathways, impairing stomatal regulation reducing water-use efficiency, while orchestrates by activating heat shock proteins other protective mechanisms. Chloroplasts, as central photosynthesis, are sensitive often exhibiting photosystem II chlorophyll degradation. Recent studies also highlight significance DDR system, genes like ZmRAD51C playing crucial maintaining genomic stability during reproductive organ development. under conditions emerges factor contributing reduced set, although precise mechanisms remain be fully elucidated. Furthermore, examines cutting-edge genetic improvement strategies, aimed at developing thermotolerant cultivars. These recent research advances underscore need for further investigation into basis thermotolerance open door future advancements breeding crops.

Язык: Английский

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Molecular Underpinnings of Plant Stress Granule Dynamics in Response to High-Temperature Stress

New Crops, Год журнала: 2025, Номер unknown, С. 100069 - 100069

Опубликована: Фев. 1, 2025

Язык: Английский

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Insights into Plant Sensory Mechanisms under Abiotic Stresses

Plants, Год журнала: 2024, Номер 13(14), С. 1907 - 1907

Опубликована: Июль 10, 2024

As sessile organisms, plants cannot survive in harmful environments, such as those characterized by drought, flood, heat, cold, nutrient deficiency, and salt or toxic metal stress. These stressors impair plant growth development, leading to decreased crop productivity. To induce an appropriate response abiotic stresses, must sense the pertinent stressor at early stage initiate precise signal transduction. Here, we provide overview of recent progress our understanding molecular mechanisms underlying stress sensing. Numerous biomolecules have been found participate process sensing function sensors plants. Based on their structure, these can be divided into four groups: Ca

Язык: Английский

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Plant Thermosensors

Annual Review of Genetics, Год журнала: 2024, Номер 58(1), С. 135 - 158

Опубликована: Июль 10, 2024

Plants are exposed to temperature conditions that fluctuate over different time scales, including those inherent global warming. In the face of these variations, plants sense adjust their functions and minimize negative consequences. Transcriptome responses underlie changes in growth, development, biochemistry (thermomorphogenesis acclimation extreme temperatures). We only beginning understand sensation by plants. Multiple thermosensors convey complementary information a given signaling network control gene expression. Temperature-induced protein or transcript structure and/or dynamics biomolecular condensates core sensing mechanisms known thermosensors, but impinges on activities via additional indirect pathways. The diversity plant anticipates many new eventually novel will be uncovered soon.

Язык: Английский

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Phytohormonal homeostasis, chloroplast stability, and heat shock transcription pathways related to the adaptability of creeping bentgrass species to heat stress

PROTOPLASMA, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Язык: Английский

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Stress resilience in plants: the complex interplay between heat stress memory and resetting

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

Summary Heat stress (HS) poses a major challenge to plants and agriculture, especially during climate change‐induced heatwaves. Plants have evolved mechanisms combat HS remember past stress. This memory involves lasting changes in specific responses, enabling better anticipate react future heat events. is multi‐layered cellular phenomenon that, addition epigenetic modifications, protein quality control, metabolic pathways broader physiological adjustments. An essential aspect of modulating timely resetting, which restores defense responses baseline levels optimizes resource allocation for growth. Balancing with resetting enables withstand while maintaining growth reproductive capacity. In this review, we discuss regulatory layers highlighting their critical balance enhancing resilience plant fitness. We primarily focus on the model Arabidopsis thaliana due limited research other species outline key areas study.

Язык: Английский

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Tackling abiotic stress in plants: recent insights and trends

Stress Biology, Год журнала: 2025, Номер 5(1)

Опубликована: Янв. 26, 2025

Abstract Plants, as sessile organisms, must adapt to a range of abiotic stresses, including drought, salinity, heat, and cold, which are increasingly exacerbated by climate change. These stresses significantly impact crop productivity, posing challenges for sustainable agriculture food security. Recent advances in omics studies genetics have shed light on molecular mechanisms underlying plant stress responses, the role calcium (Ca 2 ⁺) signaling, liquid–liquid phase separation (LLPS), cell wall-associated sensors detecting responding environmental changes. However, gaps remain understanding how rapid signaling is integrated with slower, adaptive processes. Emerging evidence also highlights crosstalk between immunity, growth regulation, mediated key components such RAF-SnRK2 kinase cascades, DELLA proteins, etc. Strategies enhance resistance without compromising yield include introducing beneficial alleles, spatiotemporal optimization decoupling from inhibition. This review emphasizes importance interdisciplinary approaches innovative technologies bridge fundamental research practical agricultural applications, aiming develop resilient crops production an era escalating challenges.

Язык: Английский

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