Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)
Опубликована: Янв. 9, 2025
Abstract Mitochondria are essential for cellular function and viability, serving as central hubs of metabolism signaling. They possess various metabolic quality control mechanisms crucial maintaining normal activities. Mitochondrial genetic disorders can arise from a wide range mutations in either mitochondrial or nuclear DNA, which encode proteins other contents. These defects lead to breakdown metabolism, such the collapse oxidative phosphorylation, one mitochondria’s most critical functions. diseases, common group disorders, characterized by significant phenotypic heterogeneity. Clinical symptoms manifest systems organs throughout body, with differing degrees forms severity. The complexity relationship between mitochondria diseases results an inadequate understanding genotype-phenotype correlation these historically making diagnosis treatment challenging often leading unsatisfactory clinical outcomes. However, recent advancements research technology have significantly improved our management conditions. translations mitochondria-related therapies actively progressing. This review focuses on physiological mitochondria, pathogenesis potential diagnostic therapeutic applications. Additionally, this discusses future perspectives diseases.
Язык: Английский
Процитировано
4
Clinical Epigenetics, Год журнала: 2025, Номер 17(1)
Опубликована: Янв. 16, 2025
Recent studies support the influence of paternal lifestyle and diet before conception on health offspring via epigenetic inheritance through sperm DNA methylation, histone modification, small non-coding RNA (sncRNA) expression regulation. Smoking may induce hypermethylation in genes related to anti-oxidation insulin resistance. Paternal obesity are associated with greater risks metabolic dysfunction alterations sperm. Metabolic changes, such as high blood glucose levels increased body weight, commonly observed fathers subjected chronic stress, addition an enhanced risk depressive-like behaviour sensitivity stress both F0 F1 generations. methylation is correlated quality ability fertilise oocytes, possibly a differentially regulated MAKP81IP3 signalling pathway. exposure toxic endocrine-disrupting chemicals (EDCs) also linked transgenerational transmission predisposition disease, infertility, testicular disorders, obesity, polycystic ovarian syndrome (PCOS) females changes during gametogenesis. As success assisted reproductive technology (ART) affected by diet, BMI, alcohol consumption, its outcomes could be improved modifying factors that dependent male choices environmental factors. This review discusses importance signatures sperm—including retention, sncRNA—for functionality, early embryo development, health. We discuss mechanisms which (obesity, smoking, EDCs, stress) impact epigenome.
Язык: Английский
Процитировано
2
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 13, 2025
Colorectal cancer (CRC) is the third most prevalent malignancy and second leading cause of cancer-related mortality worldwide, with an increasing shift towards younger age onset. In recent years, there has been recognition significance tRNA-derived small RNAs (tsRNAs), encompassing fragments (tRFs) tRNA halves (tiRNAs). Their involvement in regulating translation, gene expression, reverse transcription, epigenetics gradually come to light. Emerging research revealed dysregulation tsRNAs CRC, implicating their role CRC initiation progression, highlighting potential early diagnosis, prognosis, therapeutic strategies. Although clinical application still its stages, findings highlight a close relationship between biogenesis function tsRNAs, chemical modifications, tumor immune microenvironment (TIME). Additionally, similar other RNAs, can be effectively delivered via nanoparticles (NPs). Consequently, future should focus on elucidating concerning base TIME regulation, immunotherapy, NPs delivery systems facilitate translation.
Язык: Английский
Процитировано
1
Epigenetics & Chromatin, Год журнала: 2025, Номер 18(1)
Опубликована: Янв. 16, 2025
DNA methylation plays a crucial role in mammalian development. While methylome changes acquired the parental genomes are believed to be erased by epigenetic reprogramming, accumulating evidence suggests that sperm caused environmental factors involved disease phenotypes of offspring. These findings imply transferred embryo after reprogramming. However, our understanding this process remains incomplete. Our previous study showed arsenic exposure F0 pregnant mice paternally increased tumor incidence F2 The arsenic-exposed F1 males exhibited characteristic features, including enrichment hypomethylated cytosines at promoters retrotransposons LINEs and LTRs. Hypomethylation is potentially detrimental. Determining whether these hypomethylation important confirming molecular pathway intergenerational transmission paternal effects exposure. We investigated male embryos reprogramming reduced representation bisulfite sequencing (RRBS) allele-specific analysis. To do so, were obtained crossing control or gestationally (C3H/HeN strain) with females (C57BL/6 strain). results revealed group was globally enriched for certain genomic regions, LTR LINE, as observed group. Unexpectedly, features detected not only genome but also maternal embryos. Furthermore, found rarely occur same positions between characteristics arsenic-induced reproduced both post-epigenetic suggest re-establishment achieved collaboration other mediate region-specific changes. highlight possibility could contribute development predisposition
Язык: Английский
Процитировано
1
Trends in Biochemical Sciences, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
1
Nature Aging, Год журнала: 2025, Номер unknown
Опубликована: Март 3, 2025
Testicular aging is associated with declining reproductive health, but the molecular mechanisms are unclear. Here we generate a dataset of 214,369 single-cell transcriptomes from testicular cells 35 individuals aged 21–69, offering resource for studying and physiology. Machine learning analysis reveals stronger response in somatic compared to germ cells. Two waves aging-related changes identified: first peritubular donors their 30s, marked by increased basement membrane thickness, indicating priming state aging. In 50s, exhibit functional changes, including altered steroid metabolism Leydig immune responses macrophages. Further analyses reveal impact body mass index on spermatogenic capacity as age progresses, particularly after 45. Altogether, our findings illuminate alterations during testis relationship index, providing foundation future research potential diagnostic markers therapeutic targets. To enhance understanding human testis, authors provide map characteristics throughout lifespan. Using samples donors, they identify two examine interactions between index.
Язык: Английский
Процитировано
1
Neuropsychopharmacology, Год журнала: 2025, Номер unknown
Опубликована: Март 8, 2025
Abstract Accumulating evidence indicates that drug addiction may lead to adaptive behavioral changes in offspring, potentially due epigenetic modifications parental germline. However, the underlying mechanisms remain inadequately understood. In this study, we show paternal heroin self-administration (SA) increased heroin-seeking behavior F1 generation, when compared with offspring sired by yoke-infused control males, indicating cross-generational impact of voluntary seeking behavior. Notably, increase was replicated zygotic microinjection sperm RNAs derived from heroin-SA-experienced rats. Analysis non-coding spermatozoa revealed coordinated miRNA content between nucleus accumbens and spermatozoa. We validated restoration miR-19b downregulation RNA self-administration-experienced rats, parallel its overexpression fathers, reversed SA observed these offspring. Taken together, our findings suggest rats induces both brain miRNA, playing a critical role mediating inheritance generation.
Язык: Английский
Процитировано
1
Nature, Год журнала: 2024, Номер 630(8017), С. 571 - 573
Опубликована: Июнь 5, 2024
Язык: Английский
Процитировано
6
Molecular Cell, Год журнала: 2024, Номер 84(19), С. 3843 - 3859.e8
Опубликована: Авг. 2, 2024
Язык: Английский
Процитировано
4
Опубликована: Авг. 23, 2024
As with all new fields of discovery, work on the biological role G-quadruplexes (GQ) has produced a number results that at first glance are quite baffling, sometimes because they do not fit well together, but mostly different from commonly held expectations. Like other classes flipons, those form have repeat sequence motif enables fold. The canonical DNA (G3N1-7)3G3, where N is any nucleotide and G guanine, feature under active selection in avian mammalian genomes. involvement G-flipons genome maintenance traces back to invertebrate C. elegans ancient repair pathways. A for GQ transcription supported by observation yeast Rap1 protein binds both B-DNA, sequence-specific manner, GQ, structure-specific through same helix. Other factors (TF) also engage conformations. RNAs can modulate formation manner cellular machinery as localized TF, linking RNA world modern world. coevolution proteins studies early embryonic development, transient potentially coordinates epigenetic specification cell fate.
Язык: Английский
Процитировано
4