International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3518 - 3518

Опубликована: Апрель 9, 2025

SOS1 is a Na+/H+ antiporter found in the plant membrane of Arabidopsis thaliana and serves as major transporter that extrudes Na+ across plasma cells exchange for intracellular H+. The first 450 amino acids comprise transport domain. Using yeast heterologous expression system, we examined nine different mutations may either change specificity or improve salt tolerance. E261K had minor negative effects on ability to confer tolerance LiCl NaCl. Mutation A399V effects, lowering slightly improving NaCl tolerance, did double mutant E261KA399V. Four acid Y346 varying effects. Y346R mutation resulted improvement but not affect L375I showed impaired whereas Q362L exhibited Our results demonstrate critical ion selectivity its can dramatically Other improvements (Y346F, A399V, Y346A), leaving open possibility such might intact species.

Язык: Английский