Expert Opinion on Drug Discovery,
Год журнала:
2023,
Номер
18(11), С. 1221 - 1230
Опубликована: Авг. 17, 2023
Macromolecular
X-ray
crystallography
and
cryo-EM
are
currently
the
primary
techniques
used
to
determine
three-dimensional
structures
of
proteins,
nucleic
acids,
viruses.
Structural
information
has
been
critical
drug
discovery
structural
bioinformatics.
The
integration
artificial
intelligence
(AI)
into
shown
great
promise
in
automating
accelerating
analysis
complex
data,
further
improving
efficiency
accuracy
structure
determination.
Cell Reports,
Год журнала:
2024,
Номер
43(6), С. 114260 - 114260
Опубликована: Июнь 1, 2024
Immunotherapy
remains
underexploited
in
acute
myeloid
leukemia
(AML)
compared
to
other
hematological
malignancies.
Currently,
gemtuzumab
ozogamicin
is
the
only
therapeutic
antibody
approved
for
this
disease.
Here,
identify
potential
targets
immunotherapeutic
intervention,
we
analyze
surface
proteome
of
100
genetically
diverse
primary
human
AML
specimens
identification
cell
proteins
and
conduct
single-cell
transcriptome
analyses
on
a
subset
these
assess
antigen
expression
at
sub-population
level.
Through
comprehensive
effort,
successfully
numerous
antigens
markers
preferentially
expressed
by
primitive
cells.
Many
identified
are
targeted
antibodies
currently
under
clinical
evaluation
various
cancer
types,
highlighting
value
approach.
Importantly,
initiative
uncovers
heterogeneity
surfaceome
level,
identifies
several
characterizing
subgroups,
positions
immunotherapy
as
promising
approach
target
subgroup
specificities.
ACS Nano,
Год журнала:
2024,
Номер
18(29), С. 19200 - 19207
Опубликована: Июль 12, 2024
Simultaneous
detection
and
structural
characterization
of
protein
variants
on
a
single
platform
are
highly
desirable
but
technically
challenging.
Herein,
we
present
single-molecule
spectral
system
based
gold
plasmonic
nanopore
for
analyzing
two
peptides
their
single-point
mutated
variants.
The
enabled
the
high-throughput
acquisition
surface-enhanced
Raman
scattering
(SERS)
spectra
at
level
by
electrically
driving
analytes
into
hot
spots.
Furthermore,
statistical
method
Boolean
operations
was
developed
to
extract
prominent
features
from
fluctuated
SERS
spectra.
effects
single-amino
acid
substitutions
both
intramolecular
interactions
peptide
conformations
were
directly
characterized
system,
results
agreed
with
predictions
AlphaFold2.
This
study
highlights
mutual
benefits
spectroscopy
technology,
whereby
offers
powerful
tool
analysis
proteins.
Current Opinion in Structural Biology,
Год журнала:
2025,
Номер
90, С. 102979 - 102979
Опубликована: Янв. 7, 2025
The
mRNA
splicing
machinery
has
been
estimated
to
generate
100,000
known
protein-coding
transcripts
for
20,000
human
genes
(Ensembl,
Sept.
2024).
However,
this
set
is
expanding
with
the
massive
and
rapidly
growing
data
coming
from
high-throughput
technologies,
particularly
single-cell
long-read
sequencing.
Yet,
implications
of
complexity
at
protein
level
remain
largely
uncharted.
In
review,
we
describe
current
advances
toward
systematically
assessing
contribution
alternative
proteome
function
diversification.
We
discuss
potential
challenges
using
artificial
intelligence-based
techniques
in
identifying
proteoforms
characterising
their
structures,
interactions,
functions.
Biomolecules,
Год журнала:
2025,
Номер
15(1), С. 130 - 130
Опубликована: Янв. 15, 2025
Proteomics
accelerates
diagnosis
and
research
of
muscular
diseases
by
enabling
the
robust
analysis
proteins
relevant
for
manifestation
neuromuscular
in
following
aspects:
(i)
evaluation
effect
genetic
variants
on
corresponding
protein,
(ii)
prediction
underlying
defect
based
proteomic
signature
muscle
biopsies,
(iii)
pathophysiologies
different
entities
diseases,
key
definition
new
intervention
concepts,
(iv)
patient
stratification
according
to
biochemical
fingerprints
as
well
(v)
monitoring
success
therapeutic
interventions.
This
review
presents—also
through
exemplary
case
studies—the
various
advantages
mass
proteomics
investigation
discusses
technical
limitations,
provides
an
outlook
possible
future
application
concepts.
Hence,
is
excellent
large-scale
analytical
tool
diagnostic
workup
(hereditary)
warrants
systematic
profiling
pathophysiological
processes.
The
steady
development
may
allow
overcome
existing
limitations
including
a
quenched
dynamic
range
quantification
protein
isoforms.
Future
directions
include
targeted
settings
using
not
only
biopsies
but
also
liquid
address
need
minimally
invasive
procedures.
Expert Opinion on Drug Discovery,
Год журнала:
2023,
Номер
18(11), С. 1221 - 1230
Опубликована: Авг. 17, 2023
Macromolecular
X-ray
crystallography
and
cryo-EM
are
currently
the
primary
techniques
used
to
determine
three-dimensional
structures
of
proteins,
nucleic
acids,
viruses.
Structural
information
has
been
critical
drug
discovery
structural
bioinformatics.
The
integration
artificial
intelligence
(AI)
into
shown
great
promise
in
automating
accelerating
analysis
complex
data,
further
improving
efficiency
accuracy
structure
determination.
