Tissue Clearing and Its Application in Nanoparticle Development
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 3, 2025
Abstract
Nanoparticles
are
widely
used
in
biomedical
applications
due
to
their
small
size,
large
surface
area,
and
unique
physicochemical
properties.
These
characteristics
make
them
ideal
for
drug
delivery,
diagnostic
imaging,
therapeutic
interventions.
Their
ability
interact
with
biological
systems
at
the
cellular
molecular
levels
enables
targeted
treatments.
Understanding
biodistribution
of
nanoparticles
level
within
whole
organisms
is
crucial
assessing
safety
effectiveness;
however,
proper
technologies
have
been
lacking
achieve
this.
For
example,
traditional
imaging
techniques
like
magnetic
resonance
(MRI)
computed
tomography
(CT)
often
lack
resolution
needed,
while
tissue‐section‐based
methods
miss
whole‐body
systemic
view.
Recent
tissue
clearing
emerged
as
a
promising
solution
3D
visualization
entire
organs,
they
enable
cellular‐level
without
need
sectioning.
This
review
explores
advancements
diverse
application
studying
nanoparticle
biodistribution,
providing
insights
development
nanoparticle‐based
therapies.
Язык: Английский
Single-Frame Vignetting Correction for Post-Stitched-Tile Imaging Using VISTAmap
Nanomaterials,
Год журнала:
2025,
Номер
15(7), С. 563 - 563
Опубликована: Апрель 7, 2025
Stimulated
Raman
Scattering
(SRS)
nanoscopy
imaging
offers
unprecedented
insights
into
tissue
molecular
architecture
but
often
requires
stitching
multiple
high-resolution
tiles
to
capture
large
fields
of
view.
This
process
is
time-consuming
and
frequently
introduces
vignetting
artifacts—grid-like
intensity
fluctuations
that
degrade
image
quality
hinder
downstream
quantitative
analyses
processing
such
as
super-resolution
deconvolution.
We
present
VIgnetted
Stitched-Tile
Adjustment
using
Morphological
Adaptive
Processing
(VISTAmap),
a
simple
tool
corrects
these
shading
artifacts
directly
on
the
final
stitched
image.
VISTAmap
automatically
detects
tile
grid
configuration
by
analyzing
frequency
variations
then
applies
sequential
morphological
operations
homogenize
In
contrast
conventional
approaches
require
increased
overlap
or
pre-acquisition
background
sampling,
pragmatic,
post-processing
solution
without
need
for
separate
individual
images.
work
addresses
pressing
concerns
delivering
robust,
efficient
strategy
enhancing
mosaic
uniformity
in
modern
nanoscopy,
where
smallest
details
make
tremendous
impacts.
Язык: Английский